Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,023,628	+G	coding (256/2154 nt)	yccS ←	putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,023,625	1	.	G	100.0%	147.2 / NA	42	W87R (TGG→CGG)	yccS	putative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/20);  total (22/20)

AGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCA‑GGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAG  >  NC_000913/1023478‑1023766                                                                                                                                                     |                                                                                                                                              aGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAgccagggcca‑g                                                                                                                                              <  7:59178/149‑1 (MQ=255)      tCATACAGTGATGTTCACAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGAAAATCGCAAATAGccagggcca‑gggga                                                                                                                                          >  6:270635/1‑146 (MQ=255)           cAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGc                                                                                                                                     <  6:262431/149‑1 (MQ=255)            aGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGc                                                                                                                                     >  7:364464/1‑148 (MQ=255)              tGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAAt                                                                                                                                  >  4:171509/1‑149 (MQ=255)                aTGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAgccagggcca‑gg                                                                                                                                             >  7:279079/1‑135 (MQ=255)                aTGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAgccagggcca‑gg                                                                                                                                             <  8:279079/135‑1 (MQ=255)                  gTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTa                                                                                                                              >  3:308208/1‑149 (MQ=255)                   ttCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTa                                                                                                                              <  5:1908/148‑1 (MQ=255)                      ccAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTg                                                                                                                           <  4:308208/148‑1 (MQ=255)                         aCATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGc                                                                                                                       <  1:1069/149‑1 (MQ=255)                              gtgtAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAgg                                                                                                                  >  6:145860/1‑149 (MQ=255)                              gtgtAAATGGCGATCAGCAATGCACCGAAGGCAATTGTAGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAACCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAgg                                                                                                                  <  3:220044/149‑1 (MQ=255)                                  aaaTGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGa                                                                                                               >  1:49708/1‑148 (MQ=255)                                       gccaTCAGCAATGCACCGAAGGCAATTGTTTCATAGGGTTGACCCAGCCCGCCGAGCAAAATTAAGCCGCTGGTAGAGATCGTTAAGCCAATCGCAAATATCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATaaaaa                                                                                                         <  5:270635/146‑1 (MQ=255)                                        cGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATaaaaaa                                                                                                        <  5:145860/149‑1 (MQ=255)                                         gATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAg                                                                                                       >  8:300022/1‑149 (MQ=255)                                          aTCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTACGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAg                                                                                                       >  8:382425/1‑148 (MQ=255)                                               cAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGaa                                                                                                  >  3:176927/1‑148 (MQ=255)                                                aaTGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACa                                                                                                <  6:274676/149‑1 (MQ=255)                                                 aTGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAg                                                                                               >  7:264091/1‑149 (MQ=255)                                                 aTGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAg                                                                                               >  5:197244/1‑149 (MQ=255)                                                     aCCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAgccagggcca‑g                                                                                                                                              <  6:383586/97‑1 (MQ=255)                                                     aCCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAgccagggcca‑g                                                                                                                                              >  5:383586/1‑97 (MQ=255)                                                     aCCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAATAAGCAGAACAGCGt                                                                                            >  7:158187/1‑148 (MQ=255)                                                       cGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAat                                                                                         <  2:195206/149‑1 (MQ=255)                                                       cGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAAAAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAat                                                                                         >  1:375034/1‑149 (MQ=255)                                                         aaGGCAATTGTTGCATAGCGTTGCCCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAatga                                                                                       >  5:47059/1‑149 (MQ=255)                                                                 tgttgCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGc                                                                                                                       <  4:216114/109‑1 (MQ=255)                                                                 tgttgCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGc                                                                                                                       >  3:216114/1‑109 (MQ=255)                                                                  gttgCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTAc                                                                              >  6:46653/1‑149 (MQ=255)                                                                  gtagaaTAGCGTTTACCCAGACCGCCGAGCAAAATGACGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTAc                                                                              >  8:194399/6‑149 (MQ=255)                                                                        tAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAAc                                                                        <  6:197244/149‑1 (MQ=255)                                                                          gggTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTTAGACAATCGCAAATAGCCAGGGCCAGGGGAATCAGCAATTCTACTTAGGCCGAGGCGATAAAAAAGCAGAAAATCTTAATGATGAGGTTAATCAAACGt                                                                      >  3:17016/3‑149 (MQ=255)                                                                                 cccAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCa                                                               <  7:382425/149‑1 (MQ=255)                                                                                    aGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTc                                                            >  5:329559/1‑149 (MQ=255)                                                                                          ccgAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCAt                                                      >  4:227746/1‑149 (MQ=255)                                                                                             aGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCga                                                   <  3:171509/149‑1 (MQ=255)                                                                                               cAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTccc                                                                   >  8:107945/1‑131 (MQ=255)                                                                                               cAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTccc                                                                   <  7:107945/131‑1 (MQ=255)                                                                                                  aaTGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCg                                              <  7:300022/149‑1 (MQ=255)                                                                                                    tGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGt                                            <  2:49708/149‑1 (MQ=255)                                                                                                            cTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGcgccg                                    <  5:203336/149‑1 (MQ=255)                                                                                                                         ttAAGCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATccc                       <  8:364464/149‑1 (MQ=255)                                                                                                                             gCCAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGc                   >  4:239964/1‑149 (MQ=255)                                                                                                                              ccAATCGCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCg                  <  6:329559/149‑1 (MQ=255)                                                                                                                                    gCAAATAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCg            <  7:298337/149‑1 (MQ=255)                                                                                                                                      aaaTAGCCAGGGCCAGGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCgg           >  8:219268/1‑148 (MQ=255)                                                                                                                                              agggccagggGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGTCACCATCCCCAGCGTTAGCGGAATCGTCAg  >  8:38564/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                              AGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCA‑GGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAG  >  NC_000913/1023478‑1023766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑