Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,722,492	C→A	T847K (ACG→AAG)	pka →	protein lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,722,492	0	C	A	100.0%	89.4 / NA	26	T847K (ACG→AAG)	pka	protein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/10);  total (16/10)

CGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTG  >  NC_000913/2722355‑2722633                                                                                                                                          |                                                                                                                                              cGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAAc                                                                                                                                     >  6:71001/1‑148 (MQ=255)         aTAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTgg                                                                                                                             <  5:71001/148‑1 (MQ=255)                          tttGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGc                                                                                                              >  3:3229/1‑146 (MQ=255)                                      gTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGtt                                                                                               >  1:110706/1‑149 (MQ=255)                                      gTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGt                                                                                                >  5:1260/1‑148 (MQ=255)                                      gTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGt                                                                                                <  6:1260/148‑1 (MQ=255)                                          gCTCGGATCTCAAAGGGTTAGGCTTAGGTCGCCGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAAc                                                                                           >  1:81661/1‑149 (MQ=255)                                           cTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTgg                                                                                                                             >  6:90653/1‑114 (MQ=255)                                           cTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTgg                                                                                                                             <  5:90653/114‑1 (MQ=255)                                            tCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCCCGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGt                                                                                         >  3:114959/1‑149 (MQ=255)                                            tCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGt                                                                                         >  3:114958/1‑149 (MQ=255)                                              ggATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATTGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGt                                                                                         <  4:3229/147‑1 (MQ=255)                                               gATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGa                                                                                      <  2:110706/149‑1 (MQ=255)                                                     aaaGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATc                                                                                  >  6:67953/1‑147 (MQ=255)                                                     aaaGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATc                                                                                  >  4:66978/1‑147 (MQ=255)                                                                cTTAGGTCGACGCTTAATGGAAAAGCTGATTACCTATACGCGAGATCACGGACTACCACGTCTGCATGGTCTTAAGATGCCAAACAATCGTGGCATGGTGGCGCCAGCCCGCAAGCTCGGGTGCAACGCTGATATCCAGCTCGAAGCgg                                                                     <  4:52764/149‑1 (MQ=255)                                                                       cGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGt                                                              >  1:72115/1‑149 (MQ=255)                                                                                             ttACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGcc                                        >  2:23266/1‑149 (MQ=255)                                                                                                    taCGCGAGATCACTGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAgcg                                   <  4:114958/147‑1 (MQ=255)                                                                                                    taCGCGAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAgcg                                   <  4:114959/147‑1 (MQ=255)                                                                                                       gcgAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAgg                              <  3:66978/149‑1 (MQ=255)                                                                                                       gcgAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAg                               >  5:78637/1‑148 (MQ=255)                                                                                                       gcgAGATCACGGACTACAACGTCTGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACCCTAAATCTTGCCACGCGCGAg                               >  8:29160/1‑148 (MQ=255)                                                                                                         gagaTCACGGACTACAACGTATGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCTCTAGCCCGCAAGCTCGGGTTTAACGGTGATATCCAGCTCGAAGATGGGATCGTTGGGCTTACGCTAAATCTTGCGAAGCGCGAGGaa                            >  8:76492/1‑149 (MQ=255)                                                                                                                              tGAATGGTATTAAGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGaaa       >  4:65097/1‑149 (MQ=255)                                                                                                                                    gTATTAAGATGCCAAACAATCGAGGCATGGTCGCGCTAGCCCGCAAGCTCGGGTCTAACGGTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAAGCATGAGTAAGGTACTGGAAATGTTg  <  7:104287/148‑1 (MQ=255)                                                                                                                                          |                                                                                                                                              CGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTG  >  NC_000913/2722355‑2722633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑