Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 167,992 C→A S170Y (TCC→TAC)  fhuA → ferrichrome outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913167,9920CA100.0% 84.8 / NA 25S170Y (TCC→TAC) fhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/15);  total (10/15)

CTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCGGTACTGACAGCCTGTTCCAGACTGGTTTTGACTTTAGCGATTCGTTGGAT  >  NC_000913/167850‑168137
                                                                                                                                              |                                                                                                                                                 
cTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCt                                                                                                                                             >  3:10673/1‑149 (MQ=255)
      aTTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACg                                                                                                                                        <  4:10673/148‑1 (MQ=255)
              cTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCa                                                                                                                               <  6:17010/149‑1 (MQ=255)
                 tGCGGCAGAAGGCCAAATCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGt                                                                                                                             <  3:58524/148‑1 (MQ=255)
                 tGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAg                                                                                                                              <  4:18357/147‑1 (MQ=255)
                 tGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAg                                                                                                                              <  8:100811/147‑1 (MQ=255)
                              cAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGtt                                                                                                               <  5:13429/149‑1 (MQ=255)
                              cAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGtt                                                                                                               <  2:70897/149‑1 (MQ=255)
                              cAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGtt                                                                                                               >  1:70897/1‑149 (MQ=255)
                                     aGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATg                                                                                                        <  5:20468/149‑1 (MQ=255)
                                       aaTAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGt                                                                                                      >  4:107848/1‑149 (MQ=255)
                                                   aaTGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGt                                                                                            <  2:17911/147‑1 (MQ=255)
                                                                   aGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCt                                                                           >  2:27463/1‑148 (MQ=255)
                                                                         aCTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTCGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAcagaa                                                                    <  2:14204/149‑1 (MQ=255)
                                                                                taACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCa                                                               <  3:107848/147‑1 (MQ=255)
                                                                                  aCGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGtt                                                            >  1:41967/1‑148 (MQ=255)
                                                                                     aTGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGt                                                                                                      >  1:107674/1‑103 (MQ=255)
                                                                                     aTGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGt                                                                                                      <  2:107674/103‑1 (MQ=255)
                                                                                        cGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGc                                                      <  2:41967/148‑1 (MQ=255)
                                                                                           tCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCgg                                                   >  7:18964/1‑148 (MQ=255)
                                                                                                        tatGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCGGTACTGACa                                           <  7:55222/143‑1 (MQ=255)
                                                                                                        tatGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCGGTACTGACa                                           >  8:55222/1‑143 (MQ=255)
                                                                                                                            ttATGCGTGGCCCGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCGGTACTGACAGCCTGTTCCAGACTGGTTTTGACtt                  >  1:124305/1‑148 (MQ=255)
                                                                                                                                       ccGGTTTACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGCACCGCTGAAAGAAGTTCAGTTTAAAGCCGGTACTGACAGCCTGTTCCAGACTGGTTTTGACTTTAGCGATTCGtt      >  4:53113/1‑149 (MQ=255)
                                                                                                                                           tttACGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCGGTACTGACAGCCTGTTCCAGACTGGTTTTGACTTTAGCGATTCGTTGgat  <  1:27463/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
CTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATATGCTGGAACGCGCTGAAATTATGCGTGGCCCGGTTTCCGTGCTTTACGGTAAAAGCAGTCCTGGCGGCCTGTTGAATATGGTCAGCAAGCGTCCGACCACCGAACCGCTGAAAGAAGTTCAGTTTAAAGCCGGTACTGACAGCCTGTTCCAGACTGGTTTTGACTTTAGCGATTCGTTGGAT  >  NC_000913/167850‑168137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: