Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	909,353	G→A	A566V (GCG→GTG)	poxB ←	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	909,353	0	G	A	100.0%	126.3 / NA	36	A566V (GCG→GTG)	poxB	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/20);  total (16/20)

TCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAA  >  NC_000913/909215‑909486                                                                                                                                           |                                                                                                                                      tcCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGAt                                                                                                                             <  6:127145/149‑1 (MQ=255)   cccAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATc                                                                                                                            <  1:13149/148‑1 (MQ=255)   cccAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCa                                                                                                                           >  3:19945/1‑149 (MQ=255)     cAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACt                                                                                                                         <  2:132247/149‑1 (MQ=255)                  catcatCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTc                                                                                                            >  7:100627/1‑149 (MQ=255)                           ttCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCtgatga                                                                                                   >  6:6072/1‑149 (MQ=255)                                         ggCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCata                                                                                     <  5:6072/149‑1 (MQ=255)                                                tCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGc                                                                              >  6:102994/1‑149 (MQ=255)                                                  gggTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCt                                                                             >  7:88015/1‑148 (MQ=255)                                                           tATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTg                                                                   <  3:3749/149‑1 (MQ=255)                                                             tCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGc                                                                 <  5:91984/149‑1 (MQ=255)                                                                   cGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTc                                                           <  5:79650/149‑1 (MQ=255)                                                                        atcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGtt                                                      <  4:19945/149‑1 (MQ=255)                                                                        atcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGtt                                                      >  3:31668/1‑149 (MQ=255)                                                                        atcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGtt                                                      >  3:112241/1‑149 (MQ=255)                                                                          caatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGttt                                                     >  5:68364/1‑148 (MQ=255)                                                                          caatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGttt                                                     >  6:12294/1‑148 (MQ=255)                                                                             tcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATc                                                 <  8:88015/149‑1 (MQ=255)                                                                             tcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATc                                                 <  4:112241/149‑1 (MQ=255)                                                                               aTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTg                                               >  6:92411/1‑149 (MQ=255)                                                                                   cATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGt                                           <  8:100627/149‑1 (MQ=255)                                                                                   cATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGt                                           <  5:92411/149‑1 (MQ=255)                                                                                    aTGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTg                                          <  5:12294/149‑1 (MQ=255)                                                                                    aTGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTg                                          <  6:68364/149‑1 (MQ=255)                                                                                                 gACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGt                                           <  3:39532/135‑1 (MQ=255)                                                                                                 gACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGt                                           >  4:39532/1‑135 (MQ=255)                                                                                                    gggAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACtcttc                          >  6:11979/1‑149 (MQ=255)                                                                                                        aaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTg                      <  5:11979/149‑1 (MQ=255)                                                                                                              aCCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGc                                   <  3:9854/130‑1 (MQ=255)                                                                                                              aCCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGc                                   >  4:9854/1‑130 (MQ=255)                                                                                                                         cTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccac     >  4:42544/1‑149 (MQ=255)                                                                                                                           tAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccac     <  6:106387/147‑1 (MQ=255)                                                                                                                           tAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccac     >  5:106387/1‑147 (MQ=255)                                                                                                                            aGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACcaca          >  7:105378/1‑141 (MQ=255)                                                                                                                            aGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACcaca          <  8:105378/141‑1 (MQ=255)                                                                                                                             gCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGTCCACCACATCCACCaa  <  4:31668/148‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      TCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAA  >  NC_000913/909215‑909486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑