Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,175,151 (A)6→5 intergenic (‑125/‑237) coaA ← / → thrU pantothenate kinase/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,175,1460A.97.8% 399.4 / 1.3 89intergenic (‑120/‑242)coaA/thrUpantothenate kinase/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base . (49/38);  total (49/40)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

CGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTC  >  NC_000913/4174997‑4175294
                                                                                                                                                     |                                                                                                                                                     
cGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATgaga                                                                                                                                                       >  2:1038478/1‑150 (MQ=255)
      tAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaaaaatc                                                                                                                                                 >  2:155353/1‑148 (MQ=255)
      tAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaaaaatc                                                                                                                                                 >  2:155457/1‑148 (MQ=255)
      tAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaaaaatc                                                                                                                                                 <  2:227016/150‑3 (MQ=255)
         cGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATctct                                                                                                                                             <  2:790198/150‑1 (MQ=255)
            ttGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGcc                                                                                                                                          <  3:293526/150‑1 (MQ=255)
             tGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCa                                                                                                                                         <  2:142505/150‑1 (MQ=255)
                tctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGa                                                                                                                                      >  2:77530/1‑150 (MQ=255)
                tctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGa                                                                                                                                      >  3:344857/1‑150 (MQ=255)
                tctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGa                                                                                                                                      <  1:971042/150‑1 (MQ=255)
                  ttttATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTATGCCAGGacg                                                                                                                                    >  2:611811/1‑148 (MQ=255)
                         cTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGt                                                                                                                             >  2:1290710/1‑150 (MQ=255)
                          tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGtt                                                                                                                            <  1:1290710/150‑1 (MQ=255)
                          tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGtt                                                                                                                            >  2:200460/1‑150 (MQ=255)
                          tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGtt                                                                                                                            >  1:1035184/1‑150 (MQ=255)
                          tCATAAGCATGTTTCTGGCGGTCATAGCCCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGtt                                                                                                                            >  1:560603/1‑150 (MQ=255)
                            aTAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGa                                                                                                                          <  1:1004636/150‑1 (MQ=255)
                              aaGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGaaa                                                                                                                        <  2:988487/150‑1 (MQ=255)
                               aGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGaaaa                                                                                                                       <  2:157077/150‑1 (MQ=255)
                                   tGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCaa                                                                                                                   >  1:8490/1‑150 (MQ=255)
                                     tttCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtg                                                                                                                 >  2:152875/1‑150 (MQ=255)
                                      ttCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtgt                                                                                                                >  1:563125/1‑150 (MQ=255)
                                        cTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAAAAAAAAAAAAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGa                                                                                                              <  4:86958/150‑1 (MQ=255)
                                         tGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGAc                                                                                                             <  4:138069/150‑1 (MQ=255)
                                         tGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGAc                                                                                                             >  4:275946/1‑150 (MQ=255)
                                          ggCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACa                                                                                                            <  2:793967/150‑1 (MQ=255)
                                           gCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAg                                                                                                           >  1:1256059/1‑150 (MQ=255)
                                            cGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGa                                                                                                          >  2:895971/1‑150 (MQ=255)
                                               tCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGatat                                                                                                       <  1:798350/150‑1 (MQ=255)
                                                 aTAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTc                                                                                                     >  3:137694/1‑150 (MQ=255)
                                                 aTAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTc                                                                                                     >  1:891995/1‑150 (MQ=255)
                                                  tAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTcc                                                                                                    <  1:182739/150‑1 (MQ=255)
                                                    gCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCAt                                                                                                  <  1:239854/150‑1 (MQ=255)
                                                     gcgccatggCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc                                                                                                 >  1:828528/8‑150 (MQ=255)
                                                     ctctATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc                                                                                                 <  1:953752/150‑1 (MQ=255)
                                                     aaatATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGCAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc                                                                                                 >  2:857298/4‑150 (MQ=255)
                                                      tctATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCt                                                                                                <  2:253154/150‑1 (MQ=255)
                                                         aTCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGAt                                                                                            <  1:1333925/150‑1 (MQ=255)
                                                         aTCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGAt                                                                                            <  2:130219/150‑1 (MQ=255)
                                                          tCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATa                                                                                           >  2:1001440/1‑150 (MQ=255)
                                                            ggCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaaa                                                                                         >  3:71523/1‑150 (MQ=255)
                                                             gCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaaaa                                                                                        >  1:1161006/1‑150 (MQ=255)
                                                                        tttGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGt                                                                             >  2:875249/1‑150 (MQ=255)
                                                                             cATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGCGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGc                                                                        >  1:220914/1‑150 (MQ=255)
                                                                              aTGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCa                                                                       >  2:1041979/1‑150 (MQ=255)
                                                                                 gcgcGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGa                                                                    <  3:66564/150‑1 (MQ=255)
                                                                                       cacaGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                                  >  2:18440/1‑99 (MQ=255)
                                                                                       cacaGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                                  <  1:18440/99‑1 (MQ=255)
                                                                                        acaGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAtt                                                             >  2:1372647/1‑150 (MQ=255)
                                                                                        acaGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAtt                                                             >  2:1168812/1‑150 (MQ=255)
                                                                                        acaGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAtt                                                             >  1:737232/1‑150 (MQ=255)
                                                                                        acaGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAtt                                                             >  2:1199006/1‑150 (MQ=255)
                                                                                          aGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGGCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgt                                                           >  2:1195580/1‑150 (MQ=255)
                                                                                           gTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                          <  2:293121/150‑1 (MQ=255)
                                                                                           gTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                          <  4:515859/150‑1 (MQ=255)
                                                                                             tCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAACTCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGa                                                        <  4:144838/150‑1 (MQ=255)
                                                                                               tGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGc                                                      <  3:283164/150‑1 (MQ=255)
                                                                                                 ttGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGcac                                                    >  2:1065561/1‑150 (MQ=255)
                                                                                                  tGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGcaca                                                   >  2:617849/1‑150 (MQ=255)
                                                                                                   gAATGGGTTAATGGGCAGGAGGGTAACACCAGCTGGGGAGGAATAATAAGAAAAATCTCTGCCAGGACGCTATCGCTAAAAAGCACTGTCAAAGCTATCAAATTGACTAAAAAGAATTATGGTTGAGCAGGAGCCCATTGTTGAGCACaa                                                  >  3:86424/1‑150 (MQ=255)
                                                                                                   gAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAACAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACaa                                                  >  1:1033985/1‑150 (MQ=255)
                                                                                                    aaTGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAat                                                 >  2:853365/1‑150 (MQ=255)
                                                                                                     aTGGGGTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAAGAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAAGTGACAGATATTCCATCTGATAAAAAGCATTAGGGTCTAGCAGGAGCGCATTGTTGAGCACActg                                                >  2:349000/1‑150 (MQ=255)
                                                                                                      tGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatga                                               >  1:941813/1‑150 (MQ=255)
                                                                                                      tGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAACAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatga                                               <  1:1099569/150‑1 (MQ=255)
                                                                                                         gctAATGGGCAGGAGGGTAACACCAGGCGGGGAGGACGAGTAAGAACAATCTTTGCCAGCACGCTATCGATGACGCGCGAAGAGACAGATAGTGCATCTGATAAAAAGAATTACGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGt                                            >  4:545672/3‑150 (MQ=255)
                                                                                                         gTTAATGGGCAGGAGGGTAACACCAGATGGGGAGAAAAAAAAAAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGt                                            <  2:570723/150‑1 (MQ=255)
                                                                                                          ttAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtgt                                                                                                                >  1:146335/1‑82 (MQ=255)
                                                                                                          ttAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtgt                                                                                                                <  2:146335/82‑1 (MQ=255)
                                                                                                            aaTGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATACAACGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGa                                         >  3:203224/1‑150 (MQ=255)
                                                                                                             aTGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGCAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGaa                                        >  2:432034/1‑150 (MQ=255)
                                                                                                             aTGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGaa                                        >  3:301409/1‑150 (MQ=255)
                                                                                                               gggCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATCCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGaaaa                                      >  2:907220/1‑150 (MQ=255)
                                                                                                                ggCAGGAGGGTACAAGCATAGTGCGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGaaaaa                                     <  1:76023/150‑1 (MQ=255)
                                                                                                                   aggaggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAgtg                                  >  2:1372904/1‑150 (MQ=255)
                                                                                                                    ggaggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAgtgt                                 <  1:1168812/150‑1 (MQ=255)
                                                                                                                    ggaggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAgtgt                                 <  1:331562/150‑1 (MQ=255)
                                                                                                                     gaggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGtgtg                                >  1:753486/1‑150 (MQ=255)
                                                                                                                     gaggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGtgtg                                >  4:589967/1‑150 (MQ=255)
                                                                                                                      aggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGc                               <  3:275946/150‑1 (MQ=255)
                                                                                                                      aggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGc                               <  2:368588/150‑1 (MQ=255)
                                                                                                                      aggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGc                               <  1:418597/150‑1 (MQ=255)
                                                                                                                          tAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAAt                           >  3:531662/1‑150 (MQ=255)
                                                                                                                            acacCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCt                         <  4:585873/150‑1 (MQ=255)
                                                                                                                                      gggAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGtt               <  1:155353/150‑1 (MQ=255)
                                                                                                                                      gggAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGtt               <  1:155457/150‑1 (MQ=255)
                                                                                                                                       ggagggATAATAAGAAAAATCGCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTc              <  2:1235334/150‑1 (MQ=255)
                                                                                                                                           gaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCATCCGTTCGGCt          <  2:429355/150‑1 (MQ=255)
                                                                                                                                            aataatAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAg                                                       <  1:169813/104‑1 (MQ=255)
                                                                                                                                            aataatAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAg                                                       >  2:169813/1‑104 (MQ=255)
                                                                                                                                              taataaGAAACCTCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACGGATATTCCATCTGATAGAAAGAATTATGGTGTAGCTGGCGCGCATTGTTGCGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGtt       <  2:1006976/150‑1 (MQ=255)
                                                                                                                                              taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGtt       >  3:421777/1‑150 (MQ=255)
                                                                                                                                                ataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTttc     >  1:656385/1‑150 (MQ=255)
                                                                                                                                                    aaaagaaTCTCCGCCAGGAAGCGATCGTCGAAAAGCAATGTGACAAATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTttcttc  <  2:60822/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTC  >  NC_000913/4174997‑4175294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: