Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,662,350 C→T P599S (CCC→TCC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,662,3500CT100.0% 75.7 / NA 23P599S (CCC→TCC) ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

GATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAAT  >  NC_000913/1662226‑1662483
                                                                                                                            |                                                                                                                                     
gATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAAc                                                                                                                 <  3:81523/147‑1 (MQ=255)
                gCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACgaggag                                                                                                                         <  5:123317/123‑1 (MQ=255)
                gCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACgaggag                                                                                                                         >  6:123317/1‑123 (MQ=255)
                         ttAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGccc                                                                                      >  3:40401/1‑149 (MQ=255)
                               ccAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCAAGCGAAAACGAAGGAACGTAACCCTGAGATGTCCTACTAAGAGGAGATGAAAACGACAGGGATCTTTAAGAAAAAATGCCCGgaag                                                                                 >  7:67686/1‑148 (MQ=255)
                                    cGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACt                                                                           <  4:40401/149‑1 (MQ=255)
                                                      tGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTc                                                                      <  1:116191/136‑1 (MQ=255)
                                                      tGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTc                                                                      >  2:116191/1‑136 (MQ=255)
                                                               cAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTg                                                   >  8:113914/1‑146 (MQ=255)
                                                                      cATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCggg                                                                                                          <  3:12504/84‑1 (MQ=255)
                                                                      cATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCggg                                                                                                          >  4:12504/1‑84 (MQ=255)
                                                                        tGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAgg                                       >  7:80771/1‑149 (MQ=255)
                                                                               aTCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAAccc                                >  1:57994/1‑149 (MQ=255)
                                                                                tCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCg                               >  2:91568/1‑149 (MQ=255)
                                                                                tCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGAACGGGATCTTTAAGAAAAAATGCCAGGAAGAACACTACGTCGCTTTCCTCTCATTACGTGAAGATCCACAGGCaaaacag                               >  8:84884/1‑145 (MQ=255)
                                                                                  aaaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                             <  8:80771/149‑1 (MQ=255)
                                                                                    atatCTCCATGCTAAAACGAAGTTACGTAACCCTGAGATTTCCGAATACGAGGAGATGATAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCTAACCCGTTGa                           <  7:84884/149‑1 (MQ=255)
                                                                                    atatCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGa                           <  1:91568/149‑1 (MQ=255)
                                                                                                       aaGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGaaaa                        <  6:29361/133‑1 (MQ=255)
                                                                                                       aaGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGaaaa                        >  5:29361/1‑133 (MQ=255)
                                                                                                             cGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAAt  <  7:113914/149‑1 (MQ=255)
                                                                                                                aaCCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTaa                                                                                                  >  6:51730/1‑50 (MQ=255)
                                                                                                                aaCCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTaa                                                                                                  <  5:51730/50‑1 (MQ=255)
                                                                                                                            |                                                                                                                                     
GATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAAT  >  NC_000913/1662226‑1662483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: