Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,175,151 (A)6→5 intergenic (‑125/‑237) coaA ← / → thrU pantothenate kinase/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,175,1460A.99.0% 468.1 / ‑2.5 104intergenic (‑120/‑242)coaA/thrUpantothenate kinase/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (54/49);  minor base T (1/0);  total (55/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGT  >  NC_000913/4174999‑4175288
                                                                                                                                                   |                                                                                                                                              
tCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATAGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGa                                                                                                                                                >  5:357455/1‑148 (MQ=255)
   ttAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaaaaa                                                                                                                                            >  2:63473/1‑149 (MQ=255)
      aCGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaaaaatct                                                                                                                                         >  4:232816/1‑146 (MQ=255)
      aCGGTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaaaaatct                                                                                                                                         >  4:281093/1‑146 (MQ=255)
        ttttGCTCTTTTATACTCATTAGCATGTTTCTGGCGGTAATATCTCTATCGGCAAAGCGATATTTGCCATGGCGAGTCACAGTTCTGTTGAATTGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATctct                                                                                                                                      <  5:198215/148‑1 (MQ=255)
        ctttGCTCTTTTATACGCATCAGCATGTTGCTGGCGGGCATAGCGCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAATctct                                                                                                                                      <  6:351154/148‑1 (MQ=255)
             ctctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAg                                                                                                                                 >  7:327129/1‑149 (MQ=255)
              tctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAg                                                                                                                                 >  5:246303/1‑148 (MQ=255)
              tctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAg                                                                                                                                 >  7:285261/1‑148 (MQ=255)
                ttttATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAg                                                                                                                             >  6:288326/1‑149 (MQ=255)
                  ttATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGa                                                                                                                               <  3:232816/146‑1 (MQ=255)
                  ttATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGc                                                                                                                            >  3:74969/1‑149 (MQ=255)
                    ataCTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTa                                                                                                                          >  5:241309/1‑149 (MQ=255)
                     taCTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTa                                                                                                                          >  8:343682/1‑148 (MQ=255)
                        tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGt                                                                                                                      >  2:312552/1‑149 (MQ=255)
                        tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGt                                                                                                                      >  8:270046/1‑149 (MQ=255)
                        tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGt                                                                                                                      >  5:348524/1‑149 (MQ=255)
                        tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGt                                                                                                                      >  5:376237/1‑149 (MQ=255)
                          aTAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCCTGGCGCGTCACAGTTCTGGGGAATGGGTGAATGGGAAGGAGGGTAACACCAGATGGGGAGGAATAATATTATACATGTCGGGCTGGAAGCTATCGtt                                                                                                                     >  4:232412/1‑148 (MQ=255)
                           tAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGa                                                                                                                   <  6:241309/149‑1 (MQ=255)
                              gCATTTTTCTGGCGGTCATAGCTCTATGGGCAAAGCGATATTTGCCATGGCGATTCACAGTTCTGTTGAATGGGTTAATGGGCAGTAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGaaaa                                                                                                                <  5:303163/149‑1 (MQ=255)
                              gCATGTTTCTGGCGGTCATAGCTCTATCTGCAAAGCGATATTTGCGATGGAGCGTCACTGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGATGAATAATAAGTAAAATCTCTGCCAGGTAGCTATCGTTGaaaa                                                                                                                <  3:166834/149‑1 (MQ=255)
                              gCAAGTTCCTGGCGGGCATAGCTCTCTCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAACTCTCTGCCAGGAAGCTATCGTTGaaaa                                                                                                                <  4:91226/149‑1 (MQ=255)
                               cATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGaa                                                                                                                  >  6:74119/1‑146 (MQ=255)
                                 tGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCa                                                                                                             <  8:327129/149‑1 (MQ=255)
                                  gTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCaa                                                                                                            >  2:79886/1‑149 (MQ=255)
                                   tttCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACGAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCaa                                                                                                            <  2:324676/148‑1 (MQ=255)
                                   tttCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                           <  6:348524/149‑1 (MQ=255)
                                   tttCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                           <  1:79886/149‑1 (MQ=255)
                                    ttCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtg                                                                                                          >  6:349717/1‑149 (MQ=255)
                                      cTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtgt                                                                                                         <  2:354016/148‑1 (MQ=255)
                                           ggTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGa                                                                                                   <  6:376237/149‑1 (MQ=255)
                                               aTAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATAtt                                                                                               >  2:60940/1‑149 (MQ=255)
                                               aTAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATAtt                                                                                               <  2:127638/149‑1 (MQ=255)
                                                tAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTc                                                                                              >  5:48691/1‑149 (MQ=255)
                                                tAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTc                                                                                              <  1:168137/149‑1 (MQ=255)
                                                 aGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTc                                                                                              >  6:278918/1‑148 (MQ=255)
                                                      tATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGATGAATAATAAGAAAAATCTCTTCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCt                                                                                         <  5:288326/147‑1 (MQ=255)
                                                          ggCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATa                                                                                     >  5:332568/1‑148 (MQ=255)
                                                          ggCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATa                                                                                     <  6:75979/148‑1 (MQ=255)
                                                           gCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaa                                                                                    >  8:320199/1‑148 (MQ=255)
                                                           gCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCGATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaa                                                                                    >  5:350782/1‑148 (MQ=255)
                                                                  gATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAAtt                                                                            <  4:94257/149‑1 (MQ=255)
                                                                  gATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAAtt                                                                            <  5:74119/149‑1 (MQ=255)
                                                                      tttGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATg                                                                         <  3:281093/148‑1 (MQ=255)
                                                                            aTGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCa                                                                                            <  4:84618/123‑1 (MQ=255)
                                                                            aTGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCa                                                                                            >  3:84618/1‑123 (MQ=255)
                                                                            aTGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAg                                                                   >  4:79958/1‑148 (MQ=255)
                                                                               gcgcGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAg                                                                <  6:48691/148‑1 (MQ=255)
                                                                                   gTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAgcg                                                           >  5:97725/1‑149 (MQ=255)
                                                                                    tCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAgcg                                                           >  5:334344/1‑148 (MQ=255)
                                                                                      acaGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATg                                                                         <  2:150164/132‑1 (MQ=255)
                                                                                      acaGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATg                                                                         >  1:150164/1‑132 (MQ=255)
                                                                                      acaGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCa                                                         <  3:328004/148‑1 (MQ=255)
                                                                                       caGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGAAATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAtt                                                       <  1:312552/149‑1 (MQ=255)
                                                                                          ttCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGCAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGAGCAttgtt                                                    >  1:126319/1‑149 (MQ=255)
                                                                                          ttCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCa                                                                                            <  8:65154/109‑1 (MQ=255)
                                                                                          ttCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCa                                                                                            >  7:65154/1‑109 (MQ=255)
                                                                                           tCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                    <  3:276936/148‑1 (MQ=255)
                                                                                              gTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCAATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAg                                                 >  5:19377/1‑148 (MQ=255)
                                                                                               ttGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGGAGCTATCGTTTAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGa                                                  <  5:339230/146‑1 (MQ=255)
                                                                                                tGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAATTCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGca                                               >  5:294071/1‑148 (MQ=255)
                                                                                                 gAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGcac                                              >  5:212061/1‑148 (MQ=255)
                                                                                                    tGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAg                                                                                                               <  8:147337/80‑1 (MQ=255)
                                                                                                    tGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAg                                                                                                               >  7:147337/1‑80 (MQ=255)
                                                                                                    tGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAat                                           >  5:249549/1‑148 (MQ=255)
                                                                                                      ggTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATTGTTTAGCAGGAGCGCTTTGTTGAGCACAatgat                                        >  6:365625/1‑149 (MQ=255)
                                                                                                      ggTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatgat                                        <  1:63473/149‑1 (MQ=255)
                                                                                                       gTTAATGGGCAGGAGGGTAACACGAGATGGGGAGTAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGt                                                                       <  3:321777/117‑1 (MQ=255)
                                                                                                       gTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAg                                                                                <  8:206438/108‑1 (MQ=255)
                                                                                                       gTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAg                                                                                >  7:206438/1‑108 (MQ=255)
                                                                                                       gTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                    <  3:223458/136‑1 (MQ=255)
                                                                                                       gTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                    >  4:223458/1‑136 (MQ=255)
                                                                                                       gTTAATGGGCAGGAGGGTAACACCAGATGGGGAGCAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGt                                                                       >  4:321777/1‑117 (MQ=255)
                                                                                                          aaTGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGtt                                     <  6:246303/148‑1 (MQ=255)
                                                                                                          aaTGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGtt                                     <  6:212061/148‑1 (MQ=255)
                                                                                                               gCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGaaaaa                               <  6:334344/149‑1 (MQ=255)
                                                                                                               gCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCAATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGaaaaa                               <  5:189256/149‑1 (MQ=255)
                                                                                                               gCAGGACGGTAACACCAGATCGGGAGGAATAATAAGAAAACTCTCCGCCAGGAAGCTAGCGTCCACACGCAATGTGACCGAAAATCCATCTGATAAAAAGAATTATGGCTTAGCAGGAGCGCATTGTTGAGCACAAAGATGTTGaaaaa                               <  8:285261/149‑1 (MQ=255)
                                                                                                                 aggaggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAgt                             >  8:229408/1‑149 (MQ=255)
                                                                                                                   gaggGTAACCCCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCTCATTGTTGAGCACAATGATGTAGAAAAAgtgt                           >  1:204730/1‑149 (MQ=255)
                                                                                                                   gaggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCa                                                                 >  3:311155/1‑111 (MQ=255)
                                                                                                                     gggTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGc                         >  8:116947/1‑149 (MQ=255)
                                                                                                                      ggTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGc                         >  2:59226/1‑148 (MQ=255)
                                                                                                                       gTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATAGTTGAGCACAATGATGTTGAAAAAGTGTGCt                        >  1:303512/1‑148 (MQ=255)
                                                                                                                        tAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTaa                      >  2:238711/1‑149 (MQ=255)
                                                                                                                            acCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGtgtg                          >  7:12844/1‑141 (MQ=255)
                                                                                                                            acCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGtgtg                          <  8:12844/141‑1 (MQ=255)
                                                                                                                            acCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTg                  <  6:249549/149‑1 (MQ=255)
                                                                                                                              cAGATGTGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGcc                >  3:233042/1‑149 (MQ=255)
                                                                                                                              cAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAg                                                                                <  1:280244/85‑1 (MQ=255)
                                                                                                                               aGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGccc               >  3:194016/1‑149 (MQ=255)
                                                                                                                                     ggaggaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGtt         <  6:97725/149‑1 (MQ=255)
                                                                                                                                      gaggaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGTAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCTTTc        >  4:189504/1‑149 (MQ=255)
                                                                                                                                      gaggaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTc        >  1:138281/1‑149 (MQ=255)
                                                                                                                                       aggaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATTAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCg       <  6:350782/149‑1 (MQ=255)
                                                                                                                                       aggaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCg       <  6:332568/149‑1 (MQ=255)
                                                                                                                                         gaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGc     >  7:12051/1‑149 (MQ=255)
                                                                                                                                            taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTTACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAAAGATGTTGAAAAACTGTGCTAATCTGCCCTCCGTTCGGCTGt  >  5:188417/1‑149 (MQ=255)
                                                                                                                                            taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGt  >  7:300854/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGT  >  NC_000913/4174999‑4175288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: