New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 43535087 (0.980)4 (0.050) 4/258 11.0 4.9% coding (214/420 nt) nusB transcription antitermination protein
?NC_000913 = 435366 74 (0.900)coding (230/420 nt) nusB transcription antitermination protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/435196‑435350
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tacctgtcccAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTACATCCTGTTCAGCCAGGAACTGGTATTCAACATCAG  <  NC_000913/435366‑435219
                                                                                                                                                                                                                                                                                                               
GCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATA                                                                                                                                                              <  7:135866/147‑1
GCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCGGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATAC                                                                                                                                                             <  4:257236/148‑1
                                        TTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATA                                                                                                                                                              >  8:51346/1‑107
                                        TTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATA                                                                                                                                                              <  7:51346/107‑1
                                                                   GTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCAGTTCTTCCAGCAGGCGGGACAGGTAT                                                                                                                           >  8:42579/1‑115
                                                                   GTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCAGTTCTTCCAGCAGGCGGGACAGGTAT                                                                                                                           <  7:42579/115‑1
                                                                             TGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCAGTTCTTCCAGCAGGCGGGA                                                                                                                                  >  6:190926/1‑98
                                                                             TGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCAGTTCTTCCAGCAGGCGGGA                                                                                                                                  <  5:190926/98‑1
                                                                                                                                                 TACCTGTCCCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTT                                        <  5:193788/120‑1
                                                                                                                                                 TACCTGTCCCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTT                                        >  6:193788/1‑120
                                                                                                                                                  ACCTGTCCCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTAC                                     >  4:306177/1‑122
                                                                                                                                                  ACCTGTCCCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTAC                                     <  3:306177/122‑1
                                                                                                                                                   CCTGTCCCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTACATCCTGTTCAGCCAGGAACTGGTATTC          >  8:215244/1‑148
                                                                                                                                                        CCCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTACATC                                  >  4:206602/1‑119
                                                                                                                                                        CCCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTACATC                                  <  3:206602/119‑1
                                                                                                                                                         CCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTACATCCTGTTCAGCCAGGAACTGGTATTCAACATCA   <  1:213888/149‑1
                                                                                                                                                         CCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTACATCCTGTTCAGCCAGGAACTGGTATTCAACATCA   >  4:245193/1‑149
                                                                                                                                                         CCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTACATCCTGTTCAGCCAGGAACTGGTATTCAACATCA   >  4:202584/1‑149
                                                                                                                                                         CCAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTACATCCTGTTCAGCCAGGAACTGGTATTCAACAT     >  7:290321/1‑147
                                                                                                                                                          CAGTTCTTCCAGCAGGCGCGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGACAACGTCTTTTACATCCTGTTCAGCCAGGAACTGGTATTCAACATCAG  >  4:352638/1‑149
                                                                                                                                                                                                                                                                                                               
GCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/435196‑435350
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tacctgtcccAGTTCTTCCAGCAGGCGGGACAGGTATGGCTTCATCAGTCCGTCGAGGTATGCGGTATTAGTCGCCACCCCGGCCAGCAGCTCACGGAAGTACAGGACGTCAACGTCTTTTACATCCTGTTCAGCCAGGAACTGGTATTCAACATCAG  <  NC_000913/435366‑435219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.