Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,121,583	0	G	.	22.2%	45.3 / 10.4	18	intergenic (‑29/+26)	wcaJ/cpsG	colanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (8/6);  new base . (2/2);  total (10/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTA  >  NC_000913/2121445‑2121723                                                                                                                                           |                                                                                                                                              cAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTa                                                                                                                                    <  2:113275/149‑1 (MQ=255)      cAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCC‑CACTGCGGGTAAgggg                                                                                                                               >  6:63368/1‑148 (MQ=255)      cAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCC‑CACTGCGGGTAAgggg                                                                                                                               <  5:63368/149‑2 (MQ=255)                             tgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAg                                                                                                         >  3:97125/1‑148 (MQ=255)                                         aaaaGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagca                                                                                              >  3:159503/1‑147 (MQ=255)                                         aaaaGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATCAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACa                                                                                                                     >  6:59794/1‑124 (MQ=255)                                         aaaaGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATCAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACa                                                                                                                     <  5:59794/124‑1 (MQ=255)                                                   cACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAg                                                                                                                                  >  2:177179/1‑101 (MQ=255)                                                                     tGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgc                                                                                      <  5:171986/127‑1 (MQ=255)                                                                     tGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgc                                                                                      >  6:171986/1‑127 (MQ=255)                                                                                                                    ttCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTc                                                >  4:116290/1‑118 (MQ=255)                                                                                                                    ttCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTc                                                <  3:116290/118‑1 (MQ=255)                                                                                                                             atcttcccTTACCC‑CACTGCGGGTAAGGGGAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAg              <  7:18674/138‑1 (MQ=255)                                                                                                                             atcttcccTTACCC‑CACTGCGGGTAAGGGGAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAg              >  8:18674/6‑143 (MQ=255)                                                                                                                                    cTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCGCATTCAGGCGCTCCACCGGGTCGGTATTGGAGGTGCGCAGGTTa  >  7:151500/1‑148 (MQ=255)                                                                                                                                    cTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGgc                                     <  4:156647/113‑1 (MQ=255)                                                                                                                                         ccGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTa                    >  4:47545/1‑125 (MQ=255)                                                                                                                                         ccGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTa                    <  3:47545/125‑1 (MQ=255)                                                                                                                                           |                                                                                                                                              CAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTA  >  NC_000913/2121445‑2121723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑