| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,255,991 | 0 | A | T | 20.0% | 62.7 / 11.9 | 30 | K213* (AAA→TAA) | yeiL | putative transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (12/12); new base T (2/4); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGT > NC_000913/2255852‑2256131 | aGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTcc < 8:54817/148‑1 (MQ=255) ggCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgg < 3:62207/149‑1 (MQ=255) ggCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCg < 1:165557/148‑1 (MQ=255) aCATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaa > 5:125468/1‑148 (MQ=255) aTCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaaaaa < 7:135004/149‑1 (MQ=255) ttttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatta > 2:183662/1‑149 (MQ=255) tatGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTAtttt < 1:183662/148‑1 (MQ=255) tatGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTAtttt < 6:125468/148‑1 (MQ=255) ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc > 4:41278/1‑112 (MQ=255) ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc < 3:41278/112‑1 (MQ=255) aGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTAc > 1:66054/1‑148 (MQ=255) aTCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATg < 6:166029/114‑1 (MQ=255) aTCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATg > 5:166029/1‑114 (MQ=255) aTCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATc > 6:58965/1‑148 (MQ=255) ctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCg < 2:96337/67‑1 (MQ=255) ctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCg > 1:96337/1‑67 (MQ=255) cATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCa > 7:1361/1‑149 (MQ=255) aaaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTa < 2:66054/148‑1 (MQ=255) aCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCt > 5:157004/1‑109 (MQ=255) aCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCt < 6:157004/109‑1 (MQ=255) aaaGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTc < 3:78530/124‑1 (MQ=255) aaaGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTc > 4:78530/1‑124 (MQ=255) ggATTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATT‑CCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCgg > 2:66920/1‑149 (MQ=255) cTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTc > 4:119435/1‑149 (MQ=255) gagaTGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATT‑CCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCAc < 1:66920/149‑1 (MQ=255) tcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtttt < 2:195935/83‑1 (MQ=255) tcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtttt > 1:195935/2‑84 (MQ=255) cGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCg > 1:8197/1‑86 (MQ=255) cGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCg < 2:8197/86‑1 (MQ=255) cGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGt < 3:119435/149‑1 (MQ=255) | AGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGT > NC_000913/2255852‑2256131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |