Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,756,5630AG26.7% 42.3 / 16.8 30intergenic (‑52/+113)aldB/yiaYaldehyde dehydrogenase B/L‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (11/11);  new base G (4/4);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCCAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTC  >  NC_000913/3756422‑3756708
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gccAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGc                                                                                                                                            <  6:102935/149‑1 (MQ=255)
   aaTAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGCGACTGGCGAAGATTTCGCCAg                                                                                                                                         >  3:87091/1‑149 (MQ=255)
         gTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGCGACTGGCGAAGATTTCGCCAGTcacgt                                                                                                                                   <  4:87091/149‑5 (MQ=255)
                         tttAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGCGACTGGCGAAGATTTCGCCAGTcac                                                                                                                                     <  8:63424/131‑3 (MQ=255)
                         tttAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGCGACTGGCGAAGATTTCGCCAGTcac                                                                                                                                     >  7:63424/1‑129 (MQ=255)
                                 gAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCAc                                                                                                             <  6:117186/147‑1 (MQ=255)
                                     gggAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAAtt                                                                                                        >  5:120105/1‑148 (MQ=255)
                                      ggAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGTca                                                                                                                                      <  3:137005/117‑2 (MQ=255)
                                      ggAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGTca                                                                                                                                      >  4:137005/1‑116 (MQ=255)
                                            cATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCa                                                                                                                                          <  1:19613/107‑1 (MQ=255)
                                            cATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCa                                                                                                                                          >  2:19613/1‑107 (MQ=255)
                                              tACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGc                                                                                                                   >  6:145397/1‑128 (MQ=255)
                                              tACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGc                                                                                                                   <  5:145397/128‑1 (MQ=255)
                                                        gCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACa                                                                                       >  7:47088/1‑146 (MQ=255)
                                                         cTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACa                                                                                       >  8:160343/1‑145 (MQ=255)
                                                          ttAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAgaga                                                                                   >  4:95401/1‑148 (MQ=255)
                                                            aaTCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGAt                                                                                  <  8:47088/147‑1 (MQ=255)
                                                                tgtgCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCtt                                                                                                                            >  2:113075/1‑101 (MQ=255)
                                                                tgtgCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCtt                                                                                                                            <  1:113075/101‑1 (MQ=255)
                                                                       aaGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCg                                                                     <  6:120105/149‑1 (MQ=255)
                                                                                tattGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAAt                                                            >  4:129928/1‑149 (MQ=255)
                                                                                  ttGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAAttt                                                          <  7:160343/149‑1 (MQ=255)
                                                                                              gTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGAc                                                                                                                      >  8:82229/1‑77 (MQ=255)
                                                                                              gTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGAc                                                                                                                      <  7:82229/77‑1 (MQ=255)
                                                                                                               ggTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACAt                             <  3:95401/149‑1 (MQ=255)
                                                                                                                          ggTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGAt                  <  3:129928/149‑1 (MQ=255)
                                                                                                                                 ccgACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTa                                                                                         <  2:1550/69‑1 (MQ=37)
                                                                                                                                 ccgACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTa                                                                                         >  1:1550/3‑71 (MQ=37)
                                                                                                                                    aCTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCca         >  5:126351/1‑148 (MQ=255)
                                                                                                                                          gAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATttc  >  1:61137/1‑149 (MQ=255)
                                                                                                                                           aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATc             <  2:68753/137‑1 (MQ=255)
                                                                                                                                           aaaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATc             >  1:68753/1‑137 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
GCCAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTC  >  NC_000913/3756422‑3756708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑