Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,662,350 C→T P599S (CCC→TCC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,662,3500CT100.0% 92.7 / NA 28P599S (CCC→TCC) ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/13);  total (15/13)

CTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAAT  >  NC_000913/1662223‑1662483
                                                                                                                               |                                                                                                                                     
cTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGtcc                                                                                                                                     >  4:113683/1‑130 (MQ=255)
cTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGtcc                                                                                                                                     <  3:113683/130‑1 (MQ=255)
         gAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGgaga                                                                                                                        <  8:103768/134‑1 (MQ=255)
         gAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGgaga                                                                                                                        >  7:103768/1‑134 (MQ=255)
                                    aGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACgag                                                                                                                            >  4:149296/1‑103 (MQ=255)
                                    aGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACgag                                                                                                                            <  3:149296/103‑1 (MQ=255)
                                                aaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCt                                                                                                      >  6:182734/1‑113 (MQ=255)
                                                       aCTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGaaaa                                                                                                                  >  6:10369/1‑94 (MQ=255)
                                                       aCTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGaaaa                                                                                                                  <  5:10369/94‑1 (MQ=255)
                                                               tCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGt                                                    >  2:88612/1‑148 (MQ=255)
                                                                   aGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaag                                                                                 <  6:16469/115‑1 (MQ=255)
                                                                   aGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaag                                                                                 >  5:16469/1‑115 (MQ=255)
                                                                     tCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaa                                                                                             >  2:44631/1‑101 (MQ=255)
                                                                     tCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaa                                                                                             <  1:44631/101‑1 (MQ=255)
                                                                        gcttGAATGTTTCAATTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGTATATTAACACGACCGGGATCTTTAAGAAAAAATGCCCGgaa                                                                                  <  2:79774/106‑1 (MQ=255)
                                                                         cATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCca                                           >  5:145756/1‑147 (MQ=255)
                                                                                 gATCAAATATCTCCATGCGAAAACGAAGGAACGTAACACTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAAcc                                 >  5:92977/1‑149 (MQ=255)
                                                                                      aaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGt                                                                       >  1:24238/1‑106 (MQ=255)
                                                                                      aaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGt                                                                       <  2:24238/106‑1 (MQ=255)
                                                                                      aaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTg                            <  6:145756/149‑1 (MQ=255)
                                                                                             ccATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAacac                     >  1:131646/1‑149 (MQ=255)
                                                                                              cATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaa                                                                                  <  2:11139/87‑1 (MQ=255)
                                                                                              cATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaa                                                                                  >  1:11139/1‑87 (MQ=255)
                                                                                              cATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAacac                     <  2:131646/148‑1 (MQ=255)
                                                                                                        cGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGaaa          <  6:92977/149‑1 (MQ=255)
                                                                                                                cGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAAc                                  <  4:121194/117‑1 (MQ=255)
                                                                                                                cGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTGTCCGCGCCTTCCGTGAAGATCCACAGGCaacc                                  >  3:121194/1‑115 (MQ=255)
                                                                                                                 gTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAAt  >  4:12733/1‑148 (MQ=255)
                                                                                                                       cTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaa                                                                                             <  5:75912/51‑1 (MQ=255)
                                                                                                                       cTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaa                                                                                             >  6:75912/1‑51 (MQ=255)
                                                                                                                               |                                                                                                                                     
CTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAAT  >  NC_000913/1662223‑1662483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: