New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4219847	45 (1.050)	6 (0.150)	6/218	3.8	13.4%	coding (1252/1737 nt)	aceK	isocitrate dehydrogenase kinase/phosphatase
	?	NC_000913	= 4219854	37 (0.960)					coding (1259/1737 nt)	aceK	isocitrate dehydrogenase kinase/phosphatase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4219695‑4219847 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atattgagcggcACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGGGTGT  <  NC_000913/4219854‑4219707                                                                                                                                                                                                                                                                                                               GCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAG                                                                                                                                                          >  6:213676/1‑149     AATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTAT                                                                                                                                                                <  1:112332/139‑1     AATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTAT                                                                                                                                                                >  2:112332/1‑139                                                 GGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGA                                                                                                                              >  7:116482/1‑129                                                 GGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGA                                                                                                                              <  8:116482/129‑1                                                                                               GGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAAT                                                                                                                                     >  7:155406/1‑76                                                                                               GGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAAT                                                                                                                                     <  8:155406/76‑1                                                                                                         ACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGG                                                                                                                           <  7:41174/76‑1                                                                                                         ACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGG                                                                                                                           >  8:41174/1‑76                                                                                                                                                 TTGCGCGGCACCATCCGCCGCTCAATATAAAGATAGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAA                                            >  8:181679/1‑115                                                                                                                                                 TTGAGCGGCACCATCCGCCGCTCAATATAAAGATAGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAA                                            <  7:181679/115‑1                                                                                                                                                     GCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGG      <  2:40141/149‑1                                                                                                                                                     GCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGG      <  4:28550/149‑1                                                                                                                                                      CGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGG      <  5:119532/148‑1                                                                                                                                                      CGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGG      >  6:119532/1‑148                                                                                                                                                         CACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGGGTGT  >  2:200799/1‑149                                                                                                                                                                                                                                                                                                               GCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4219695‑4219847 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atattgagcggcACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGGGTGT  <  NC_000913/4219854‑4219707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.