breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,806,340 C→T intergenic (‑257/‑30) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
RA 1,807,103 C→A P245Q (CCG→CAG)  pfkB → 6‑phosphofructokinase II
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1311639 1311665 27 5 [3] [4] 6 tonB membrane spanning protein in TonB‑ExbB‑ExbD transport complex
* * ÷ NC_000913 2066270–2066600 2066600 1–331 5 [4] [3] 5 [insH1] [insH1]
* * ÷ NC_000913 3423765–3424234 3424515–3424238 5–751 7 [4] [2] 5 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4107560 4108491 932 5 [1] [0] 6 pfkA 6‑phosphofructokinase I

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 225468 =2 (0.070)9 (0.330) 11/204 1.3 83.0% intergenic (+11/‑32) ileV/alaV tRNA‑Ile/tRNA‑Ala
?NC_000913 = 3427068 NA (NA)intergenic (‑35/+8) alaU/ileU tRNA‑Ala/tRNA‑Ile
* ? NC_000913 = 225895NA (NA)11 (0.390) 9/208 1.7 NA noncoding (137/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 = 225899 NA (NA)noncoding (141/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 = 228108NA (NA)4 (0.140) 4/214 3.0 NA noncoding (2350/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 = 228124 NA (NA)noncoding (2366/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 274620 =NA (NA)4 (0.140) 4/204 2.9 NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 382282 =NA (NA)4 (0.140) 4/210 3.0 NA noncoding (1023/1331 nt) IS2 repeat region
?NC_000913 382313 = NA (NA)noncoding (1054/1331 nt) IS2 repeat region
* ? NC_000913 = 51944217 (0.570)6 (0.210) 5/206 2.6 25.1% coding (324/627 nt) tesA acyl‑CoA thioesterase 1 and protease I and lysophospholipase L1
?NC_000913 = 519458 20 (0.720)coding (308/627 nt) tesA acyl‑CoA thioesterase 1 and protease I and lysophospholipase L1
* ? NC_000913 = 82399312 (0.400)6 (0.210) 6/210 2.4 33.0% coding (505/762 nt) ybhP endo/exonuclease/phosphatase family protein
?NC_000913 = 824001 13 (0.460)coding (497/762 nt) ybhP endo/exonuclease/phosphatase family protein
* ? NC_000913 = 85919229 (0.960)7 (0.250) 7/206 2.1 21.2% intergenic (‑131/+21) ybiU/ybiV DUF1479 family protein/sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase
?NC_000913 = 859197 25 (0.900)intergenic (‑136/+16) ybiU/ybiV DUF1479 family protein/sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase
* ? NC_000913 974765 =11 (0.370)4 (0.140) 4/210 3.0 35.7% coding (447/1323 nt) mukF chromosome condensin MukBEF, kleisin‑like subunit, binds calcium
?NC_000913 974803 = 4 (0.140)coding (485/1323 nt) mukF chromosome condensin MukBEF, kleisin‑like subunit, binds calcium
* ? NC_000913 1207790 =3 (0.100)11 (0.430) 11/190 1.2 79.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 3 (0.120)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078053 (0.100)18 (0.700) 16/190 0.6 86.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 3 (0.120)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1269811 =22 (0.730)6 (0.210) 5/206 2.6 23.3% intergenic (‑1/‑47) ldrB/rdlB toxic polypeptide, small/sRNA antisense regulator affects LdrB translation; proposed addiction module in LDR‑B repeat, with toxic peptide LdrB
?NC_000913 1270384 = 19 (0.680)intergenic (‑39/‑9) ldrC/rdlC toxic polypeptide, small/sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC
* ? NC_000913 1633479 =0 (0.000)4 (0.140) 4/208 2.9 72.7% intergenic (+174/‑143) ydfK/pinQ cold shock protein, function unknown, Qin prophage/Qin prophage; putative site‑specific recombinase
?NC_000913 1633499 = 3 (0.110)intergenic (+194/‑123) ydfK/pinQ cold shock protein, function unknown, Qin prophage/Qin prophage; putative site‑specific recombinase
* ? NC_000913 1876115 =25 (0.830)6 (0.220) 5/204 2.6 24.0% coding (443/1182 nt) yeaN putative MFS transporter, inner membrane protein
?NC_000913 1876150 = 15 (0.540)coding (478/1182 nt) yeaN putative MFS transporter, inner membrane protein
* ? NC_000913 1941819 =30 (1.000)8 (0.290) 8/206 1.9 23.2% coding (765/933 nt) znuA zinc ABC transporter periplasmic binding protein
?NC_000913 1941845 = 25 (0.900)coding (739/933 nt) znuA zinc ABC transporter periplasmic binding protein
* ? NC_000913 = 2146653NA (NA)4 (0.140) 4/208 2.9 NA noncoding (54/84 nt) REP147 (repetitive extragenic palindromic) element; contains 2 REP sequences REP147 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913 = 2146683 NA (NA)noncoding (84/84 nt) REP147 (repetitive extragenic palindromic) element; contains 2 REP sequences REP147 (repetitive extragenic palindromic) element; contains 2 REP sequences
* ? NC_000913 2590715 =12 (0.400)5 (0.190) 5/198 2.5 25.8% intergenic (+7/+92) acrD/ypfM aminoglycoside/multidrug efflux system/stress‑induced small enterobacterial protein
?NC_000913 2590747 = 18 (0.670)intergenic (+39/+60) acrD/ypfM aminoglycoside/multidrug efflux system/stress‑induced small enterobacterial protein
* ? NC_000913 3470277 =NA (NA)6 (0.210) 5/208 2.6 NA coding (1053/1185 nt) tufA translation elongation factor EF‑Tu 1
?NC_000913 3470311 = NA (NA)coding (1019/1185 nt) tufA translation elongation factor EF‑Tu 1
* ? NC_000913 4213631 =19 (0.630)5 (0.180) 5/210 2.6 20.4% intergenic (+14/+49) yjaA/yjaB stress‑induced protein/GNAT‑family putative N‑acetyltransferase; acetyl coenzyme A‑binding protein
?NC_000913 4213662 = 21 (0.740)intergenic (+45/+18) yjaA/yjaB stress‑induced protein/GNAT‑family putative N‑acetyltransferase; acetyl coenzyme A‑binding protein