Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,319	C→T	intergenic (‑236/‑51)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,319	0	C	T	100.0%	131.4 / NA	38	intergenic (‑236/‑51)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/20);  total (18/20)

CTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAA  >  NC_000913/1806197‑1806444                                                                                                                           |                                                                                                                              cTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACt                                                                                                     >  7:90719/1‑149 (MQ=255)        aTGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAAt                                                                                                >  2:13696/1‑147 (MQ=255)           tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                            <  8:90719/148‑1 (MQ=255)           tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                            <  1:155045/148‑1 (MQ=255)               cTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGc                                                                                                                      >  4:128419/1‑118 (MQ=255)               cTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGc                                                                                                                      <  3:128419/118‑1 (MQ=255)                ttATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATag                                                                                      >  8:130871/1‑149 (MQ=255)                  aTTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCgg                                                                                                              <  4:4780/123‑1 (MQ=255)                  aTTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCgg                                                                                                              >  3:4780/1‑123 (MQ=255)                       aTTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                             <  5:75234/119‑1 (MQ=255)                            ttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                           <  1:13696/148‑1 (MQ=255)                             tGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                          <  7:130871/148‑1 (MQ=255)                                aTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTc                                                                                                                         <  2:30809/98‑1 (MQ=255)                                aTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTc                                                                                                                         >  1:30809/1‑98 (MQ=255)                                      aTGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                             <  8:18153/104‑1 (MQ=255)                                      aTGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                             >  7:18153/1‑104 (MQ=255)                                      aTGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATc                                                                 >  4:130820/1‑148 (MQ=255)                                         tgGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                            <  7:143240/118‑1 (MQ=255)                                         tgGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                            >  8:143240/1‑118 (MQ=255)                                           gCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATAc                                                            <  3:130820/148‑1 (MQ=255)                                                      tttttATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                       >  1:113517/1‑142 (MQ=255)                                                      tttttATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                       <  2:113517/142‑1 (MQ=255)                                                                            tGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaac                           >  6:26455/1‑148 (MQ=255)                                                                             gCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaacaa                         >  6:165845/1‑149 (MQ=255)                                                                               aaaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAAt                        >  6:177701/1‑148 (MQ=255)                                                                                           aaaGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCgg                                                                                                              <  6:9860/50‑1 (MQ=255)                                                                                           aaaGCTCCAATAAATCATATTGTTAATTTCGTTACTTTCCGCTGATTCgg                                                                                                              >  5:9860/1‑50 (MQ=255)                                                                                                    aTAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa  <  5:26455/149‑1 (MQ=255)                                                                                                    aTAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa  <  5:177701/149‑1 (MQ=255)                                                                                                        aTCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCtata                                                                                       <  6:52947/60‑1 (MQ=255)                                                                                                        aTCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCtata                                                                                       >  5:52947/1‑60 (MQ=255)                                                                                                         tCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTa                                                                       <  7:139016/75‑1 (MQ=255)                                                                                                         tCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTa                                                                       >  8:139016/1‑75 (MQ=255)                                                                                                             atTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCtat                                                              <  2:175192/80‑1 (MQ=255)                                                                                                              ttGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcc                    >  7:120868/1‑121 (MQ=255)                                                                                                              ttGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcc                    <  8:120868/121‑1 (MQ=255)                                                                                                                   aaTTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaac                           >  4:93643/1‑109 (MQ=255)                                                                                                                   aaTTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaac                           <  3:93643/109‑1 (MQ=255)                                                                                                                           |                                                                                                                              CTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAA  >  NC_000913/1806197‑1806444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑