Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	75,953	IS2 (–) +5 bp	coding (1343‑1347/1656 nt)	sgrR ←	transcriptional DNA‑binding transcriptional activator of sgrS sRNA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	75953 =	2 (0.060)	48 (1.320)	44/250	0.0	95.8%	coding (1347/1656 nt)	sgrR	transcriptional DNA‑binding transcriptional activator of sgrS sRNA
	?	NC_000913	381260 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 75957	2 (0.060)	45 (1.250)	39/248	0.1	95.6%	coding (1343/1656 nt)	sgrR	transcriptional DNA‑binding transcriptional activator of sgrS sRNA
	?	NC_000913	= 382590	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GCCTCACCCTAACCTTTTATCAGGATCACAGTGAGCATCGGGTGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/76092‑75953 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAA  >  NC_000913/381260‑381401                                                                                                                                                                                                                                                                                            GCCTCACCCTAACCTTTTATCAGGATCACAGTGAGCATCGGGTGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGC                                                                                                                                       <  6:154236/149‑1               TTTTATCAGGATCACAGTGAGCATCGGGTGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCA                                                                                                                         >  7:25887/1‑149               TTTTATCAGGATCACAGTGAGCATCGGGTGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTC                                                                                                                           >  6:179931/1‑147                   ATCAGGATCACAGTGAGCATCGGGTGCTTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGCCTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACA                                                                                                                     >  7:150798/1‑149                    TCAGGATCACAGTGAGCATCGGGTGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGA                                                                                                                                            >  8:59290/1‑125                    TCAGGATCACAGTGAGCATCGGGTGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGA                                                                                                                                            <  7:59290/125‑1                      AGGATCACAGTGAGCATCGGGTGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAAC                                                                                                                   >  1:124516/1‑148                         ATCACAGTGAGCATCGGGTGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAAT                                                                                                               <  5:179931/149‑1                             CAGTGAGCATCGTGTGATTGCCGTGTTCATGCAGCAGATTCTGGCAAGTCACCAGTTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATAGAATAGACTGGCCCCCTGAATCTCCAGACTACCAATATCA                                                                                                           <  8:150798/149‑1                                TGAGCATCGGGTGATTGCCGGGATCATGCACCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCAAACAGGAGAGATCGAATAGACTGGCCCCCTCAATCTCCAGACACCCAATATCACT                                                                                                         >  6:45178/1‑148                                        GGGTGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAG                                                                                                 <  2:124516/148‑1                                           TGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGA                                                                                              >  5:13167/1‑148                                              TTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCTAAGAGATCGAATACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAA                                                                                                  <  8:84457/141‑1                                              TTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAA                                                                                                  >  7:84457/1‑141                                                 CCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCT                                                                                        >  2:95092/1‑148                                                 CCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTC                                                                                         <  2:180084/147‑1                                                 CCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTC                                                                                         >  1:180084/1‑147                                                    GGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAA                                                                                                     >  6:35460/1‑132                                                    GGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAA                                                                                                     <  5:35460/132‑1                                                     GATCTTTCAGCAGATTCTGGCAAGTCACCAGGGCACGCTGTAAATCAAAGAGATCGACTACGATCAGTGGCATTCAGTAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATA                                                                                   <  5:45178/149‑1                                                            CAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTT                                                                            >  4:114663/1‑149                                                             AGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAAC                                                                                                                   >  2:201188/1‑109                                                             AGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAAC                                                                                                                   <  1:201188/109‑1                                                              GCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTAGGTTCAGTGGCATACAGGAGAGATCGAATATACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAA                                                                                     <  5:37974/138‑1                                                              GCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTT                                                                          <  8:25887/149‑1                                                              GCAGATTCTGGCAAGTCACCAGGTCACGCTCAAAAACAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGAACGAATAGACTGGCCCACTGAAACTCCAGACAACCAAAATCACTTAAATAAGTGAAAGTCTTAA                                                                                     >  6:37974/1‑138                                                                     CTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA                                                                    <  6:13167/148‑1                                                                        GCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAG                                                                   <  1:95092/146‑1                                                                             TCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGA                                                           <  3:114663/149‑1                                                                               ACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAG                                                          >  2:191081/1‑148                                                                                             AAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGAT                                            <  1:191081/148‑1                                                                                                   AAAGAGATCGACTACGATCAGTGGCATAAAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAG                                     >  6:3701/1‑149                                                                                                    AAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATT                                               <  8:10864/138‑1                                                                                                    AAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATT                                               >  7:10864/1‑138                                                                                                     AGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGG                                   >  2:201957/1‑149                                                                                                         ATCGACTACGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCATGAAGATCCAGACAACCAATATCACTTAAATAAGTGATAGACTTAATAATAGTTTTTAGACTATTCATTGGTAAACAGATGATTGATGgatta                                      >  6:116323/1‑137                                                                                                         ATCGACTAAGATCATTTGCATAAATGATATATCGTATATAATGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTA                                      <  5:116323/142‑1                                                                                                                 CGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAG                                     >  3:118573/1‑135                                                                                                                 CGATCAGTGGCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAG                                     <  4:118573/135‑1                                                                                                                          GCATACAGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCG               >  1:132750/1‑148                                                                                                                                AGGAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACA        >  1:54211/1‑149                                                                                                                                  GAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAA                          >  3:131500/1‑129                                                                                                                                  GAGAGATCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAA                          <  4:131500/129‑1                                                                                                                                        TCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAT                                                   >  7:160448/1‑98                                                                                                                                        TCGAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAT                                                   <  8:160448/98‑1                                                                                                                                          GAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAA  >  5:95575/1‑145                                                                                                                                          GAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAA  >  8:5115/1‑145                                                                                                                                          GAATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAA  >  5:153155/1‑145                                                                                                                                                                                                                                                                                            GCCTCACCCTAACCTTTTATCAGGATCACAGTGAGCATCGGGTGATTGCCGGGATCATGCAGCAGATTCTGGCAAGTCACCAGGTCACGCTGAAAATCAAAGAGATCGACTACGATCAGTGGCATACAGGAGAGATCGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/76092‑75953 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAA  >  NC_000913/381260‑381401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.