Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,806,319 C→T intergenic (‑236/‑51) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,806,3190CT98.8% 287.8 / NA 83intergenic (‑236/‑51)ydiY/pfkBacid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (39/43);  minor base A (0/1);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTG  >  NC_000913/1806173‑1806458
                                                                                                                                                  |                                                                                                                                           
taacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTAc                                                                                                                                           <  4:384761‑M2/13‑1 (MQ=255)
   cgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACttt                                                                                                                                        >  4:195111‑M2/134‑149 (MQ=255)
   cgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACttt                                                                                                                                        <  3:141612‑M2/16‑1 (MQ=255)
    gccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTc                                                                                                                                       >  5:176005‑M2/133‑149 (MQ=255)
     ccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTcc                                                                                                                                      >  2:402265‑M2/132‑149 (MQ=255)
      cttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCg                                                                                                                                     >  4:240624‑M2/131‑149 (MQ=255)
         aaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccatccTTAATTTCTTTACTTTCCGc                                                                                                                                    <  8:223219‑M2/20‑1 (MQ=255)
           atggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGAt                                                                                                                                >  6:409466‑M2/126‑149 (MQ=255)
            tggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgaggcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGAtt                                                                                                                               <  3:340361‑M2/25‑1 (MQ=255)
              gcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCg                                                                                                                             <  5:252914‑M2/27‑1 (MQ=255)
                gaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                                           >  8:77442‑M2/121‑149 (MQ=255)
                gaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                                           <  5:409466‑M2/29‑1 (MQ=255)
                gaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                                           >  1:18727‑M2/121‑149 (MQ=255)
                   gaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGcc                                                                                                                        <  8:246815‑M2/32‑1 (MQ=255)
                    aaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCa                                                                                                                       <  3:240624‑M2/33‑1 (MQ=255)
                        ggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACt                                                                                                                   >  8:60402‑M2/113‑149 (MQ=255)
                         gtctaaataggctgattcaaggcatttactggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTg                                                                                                                  <  2:18727‑M2/38‑1 (MQ=255)
                            taaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaaa                                                                                                               >  4:190444‑M2/109‑149 (MQ=255)
                              aataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTc                                                                                                                                       <  3:207857‑M2/17‑1 (MQ=255)
                              aataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTc                                                                                                                                       >  4:207857‑M2/107‑123 (MQ=255)
                              aataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAAt                                                                                                              <  8:162186‑M2/42‑1 (MQ=255)
                               ataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCa                                                                                                            <  3:195111‑M2/44‑1 (MQ=255)
                                  ggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGcc                                                                                                         <  1:68242‑M2/47‑1 (MQ=255)
                                          caaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATagga                                                                                                  <  4:165303‑M2/54‑1 (MQ=255)
                                           aaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaa                                                                                                                >  8:158676‑M2/94‑133 (MQ=255)
                                           aaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaa                                                                                                                <  7:158676‑M2/40‑1 (MQ=255)
                                           aaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCtat                                                                                                      <  2:310203‑M2/50‑1 (MQ=255)
                                           aaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCtat                                                                                                      >  1:310203‑M2/94‑143 (MQ=255)
                                           aaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggag                                                                                                 >  1:63718‑M2/94‑148 (MQ=255)
                                           aaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTGAATTTCTTTACTTTCCGCGGATGAGGTGCCAGACTGAAATCAGCCTATaggag                                                                                                 >  2:118513‑M2/94‑148 (MQ=255)
                                            aggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGc                                                                                                                                    <  1:376284‑M2/20‑1 (MQ=255)
                                            aggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGc                                                                                                                                    >  2:376284‑M2/93‑112 (MQ=255)
                                              gcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGaaa                                                                                             >  7:111032‑M2/91‑149 (MQ=255)
                                                atttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatg                                                                                           >  2:251003‑M2/89‑149 (MQ=255)
                                                atttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAat                                                                                            >  1:339760‑M2/89‑148 (MQ=255)
                                                   tacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                         <  6:174784‑M2/63‑1 (MQ=255)
                                                    acggtagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattagcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                        <  1:118513‑M2/64‑1 (MQ=255)
                                                    acgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                        <  6:176005‑M2/64‑1 (MQ=255)
                                                      gggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTa                                                                                     >  4:249742‑M2/83‑149 (MQ=255)
                                                       ggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                        >  2:398968‑M2/82‑145 (MQ=255)
                                                       ggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                        <  1:398968‑M2/64‑1 (MQ=255)
                                                       ggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAc                                                                                    <  7:77442‑M2/68‑1 (MQ=255)
                                                        gagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCtat                                                                                                      >  6:313400‑M2/81‑130 (MQ=255)
                                                        gagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCtat                                                                                                      <  5:313400‑M2/50‑1 (MQ=255)
                                                        gagaaaaaatcggctaaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACg                                                                                   >  8:222705‑M2/81‑149 (MQ=255)
                                                             aaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaacaatttccccgcttattcgcaccttccTTAATTTCTTAACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCt                                                                              <  6:294806‑M2/74‑1 (MQ=255)
                                                              aaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAc                                                                                    >  7:253536‑M2/75‑142 (MQ=255)
                                                              aaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAc                                                                                    <  8:253536‑M2/68‑1 (MQ=255)
                                                               aatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCtat                                                                            <  6:72955‑M2/76‑1 (MQ=255)
                                                                atcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCtata                                                                           >  3:367197‑M2/73‑149 (MQ=255)
                                                                      tcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                     >  4:302294‑M2/67‑149 (MQ=255)
                                                                      tcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                     >  5:379705‑M2/67‑149 (MQ=255)
                                                                        aaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                     <  3:190444‑M2/83‑1 (MQ=255)
                                                                         aacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                  >  2:257988‑M2/64‑149 (MQ=255)
                                                                          acatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAATTGATGGTTCGTATCTATACGTTGACACt                                                                 >  6:340830‑M2/63‑149 (MQ=255)
                                                                             tgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACTTTGACACTTgc                                                              >  5:299418‑M2/60‑149 (MQ=255)
                                                                               aagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgcg                                                             <  1:257988‑M2/91‑1 (MQ=255)
                                                                                 gaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGc                                                                                                                                    >  7:188614‑M2/56‑75 (MQ=255)
                                                                                 gaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGc                                                                                                                                    <  8:188614‑M2/20‑1 (MQ=255)
                                                                                  aaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGccc                                                          >  8:168389‑M2/55‑148 (MQ=255)
                                                                                  aaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCct                                                         >  2:181159‑M2/55‑149 (MQ=255)
                                                                                  aaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCct                                                         >  4:244339‑M2/55‑149 (MQ=255)
                                                                                       aaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCAGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctct                                                     <  6:379705‑M2/99‑1 (MQ=255)
                                                                                            actgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                                <  7:222705‑M2/104‑1 (MQ=255)
                                                                                            actgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAg                                               <  2:339760‑M2/105‑1 (MQ=255)
                                                                                             ctgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAgc                                              <  2:429088‑M2/106‑1 (MQ=255)
                                                                                                       cgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTa                                    <  2:224351‑M2/116‑1 (MQ=255)
                                                                                                             gaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGc                              <  8:111032‑M2/122‑1 (MQ=255)
                                                                                                                tttccccgcttattcgctccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGGCAGACTGAAATCAGCCTATAGGAGTAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCaaa                           <  4:258756‑M2/125‑1 (MQ=255)
                                                                                                                    cccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAttt                        <  3:244339‑M2/128‑1 (MQ=255)
                                                                                                                       gcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATcc                    <  3:302294‑M2/132‑1 (MQ=255)
                                                                                                                         ttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATccc                   >  2:313168‑M2/16‑148 (MQ=255)
                                                                                                                            ttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                <  1:402265‑M2/136‑1 (MQ=255)
                                                                                                                               gcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATagg                                                                                                   >  7:180930‑M2/10‑62 (MQ=255)
                                                                                                                               gcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATagg                                                                                                   <  8:180930‑M2/53‑1 (MQ=255)
                                                                                                                               gcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                >  6:171460‑M2/10‑145 (MQ=255)
                                                                                                                               gcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                <  5:171460‑M2/136‑1 (MQ=255)
                                                                                                                                 accttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGaaaa          <  5:276210‑M2/142‑1 (MQ=255)
                                                                                                                                 accttccCTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                         >  4:10673‑M2/8‑70 (MQ=255)
                                                                                                                                      ccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcg     >  8:63275‑M2/3‑149 (MQ=255)
                                                                                                                                       cTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTAGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcg     <  4:367197‑M2/147‑1 (MQ=255)
                                                                                                                                        ttAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCt   >  2:193563/1‑149 (MQ=255)
                                                                                                                                          aaTTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTg  <  1:181159/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
CGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTG  >  NC_000913/1806173‑1806458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: