Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,317,667 T→G E737A (GAA→GCA)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,317,6670TG98.8% 295.5 / NA 85E737A (GAA→GCA) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (43/41);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAAC  >  NC_000913/2317525‑2317810
                                                                                                                                              |                                                                                                                                               
acTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGACGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgt                                                                                                                                          <  1:352438/150‑1 (MQ=255)
 cTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgtt                                                                                                                                         <  1:885070/150‑1 (MQ=255)
 cTGTGTACCCACTCCCCTGGCGCTTCCTGCGGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTTCCTCGTCATTGATCAACACATCTTCGGGCGTCGGTTCCTGCCCTGCGTAtgtt                                                                                                                                         <  1:449405/150‑1 (MQ=255)
      tACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAac                                                                                                                                    >  4:110907/1‑150 (MQ=255)
           aCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAAcgacga                                                                                                                               >  3:276157/1‑150 (MQ=255)
            cTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCGCTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTGCTGCCCTGCGCATGTTGTAAcgacgag                                                                                                                              >  2:847682/1‑149 (MQ=255)
            cTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGAt                                                                                                                              <  3:330089/150‑1 (MQ=255)
              ccccTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGc                                                                                                                            <  1:1098477/150‑1 (MQ=255)
              ccccTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGc                                                                                                                            <  2:829697/150‑1 (MQ=255)
                    gcgcTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgctgc                                                                                                                      >  2:451195/1‑150 (MQ=255)
                       cTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgctgcgct                                                                                                                   <  1:960392/150‑1 (MQ=255)
                       cTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgctgcgct                                                                                                                   <  1:1128671/150‑1 (MQ=255)
                              cAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACt                                                                                                            >  1:920277/1‑150 (MQ=255)
                               aGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTg                                                                                                           >  4:471307/1‑150 (MQ=255)
                               aGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTg                                                                                                           <  1:604760/150‑1 (MQ=255)
                               aGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTg                                                                                                           >  2:448334/1‑150 (MQ=255)
                               aGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCCTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTg                                                                                                           >  4:283762/1‑150 (MQ=255)
                               aGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTCATCAACACATCTCCGGGAGACGATTCCTGCCCTGCGTCTGATGTAACGACGATGCCGCTGCGCGGCCAAcgg                                                                                                           >  2:531932/1‑148 (MQ=255)
                                gCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGCTGCCGCTGCGCTGCCAACTgg                                                                                                          >  2:71250/1‑150 (MQ=255)
                                  ggAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGGTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGtt                                                                                                        <  1:542592/150‑1 (MQ=255)
                                    aaTACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGCGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCACACTGGTTTc                                                                                                      >  1:1057016/1‑150 (MQ=255)
                                    aaTACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTc                                                                                                      >  1:323520/1‑150 (MQ=255)
                                     aTACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTcc                                                                                                     <  4:177523/150‑1 (MQ=255)
                                      tACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCa                                                                                                    <  1:253303/150‑1 (MQ=255)
                                       aCCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                   <  2:101513/150‑1 (MQ=255)
                                          aaTATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGaa                                                                                                <  2:891821/150‑1 (MQ=255)
                                            tatGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACt                                                                                              <  4:80544/150‑1 (MQ=255)
                                              tGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGa                                                                                            <  1:1044485/150‑1 (MQ=255)
                                                  gacgacAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACaga                                                                                        >  2:1053671/1‑150 (MQ=255)
                                                     gacAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCg                                                                                     >  3:314947/1‑150 (MQ=255)
                                                      acAGAAGGTCACTACCGCTCTGCCCTACCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGa                                                                                    <  1:45184/150‑1 (MQ=255)
                                                       cAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGAc                                                                                   <  4:276157/150‑1 (MQ=255)
                                                       cAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGAc                                                                                   <  3:187150/150‑1 (MQ=255)
                                                          aaGGTCACTACCGCTCTGCCCTGCCATTTTTTTTTTTTTTTTTCATCAGTGATCAACACATCTTCGGCAGTCGGTTCCTGCTCTGCTTATGTTGTAACGACGATCCCGCTGCGCTGCAAACTGGTTTCCCGGAACTATCAGTGCgcctct                                                                                >  2:436287/1‑145 (MQ=255)
                                                             ttCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGCCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTCACGAGGCTGCACCTGCGCTGCAAACTGGTTCCAAGGAACAGAAAGAGCGACGCATTg                                                                             >  1:235040/2‑150 (MQ=255)
                                                             gTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTg                                                                             <  2:1099987/150‑1 (MQ=255)
                                                               cACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCg                                                                           <  1:1053671/150‑1 (MQ=255)
                                                                aCTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCgg                                                                          <  1:451195/150‑1 (MQ=255)
                                                                     cGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCgc                                                                     <  2:615421/150‑1 (MQ=255)
                                                                       ctctGCCCTGCCATTTTTTTTTTTCTATCTCGTCAGAGAGCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCgccc                                                                   >  2:1040203/1‑149 (MQ=255)
                                                                        tctGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGATGCAAACTGGCTTCCAGGAACTGACAGAGCGACGCATTGCGGACCgccag                                                                  >  1:1102507/1‑150 (MQ=255)
                                                                         ctgccctgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGGTCgctagc                                                                 <  2:693116/150‑1 (MQ=255)
                                                                         ctgccctgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCgccagc                                                                 >  2:19299/1‑150 (MQ=255)
                                                                              ctgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCcagca                                                            <  2:1114611/150‑1 (MQ=255)
                                                                              ctgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCcagca                                                            <  2:17952/150‑1 (MQ=255)
                                                                                  cATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgct                                                                                                                        >  1:650934/1‑86 (MQ=255)
                                                                                  cATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgct                                                                                                                        <  2:650934/86‑1 (MQ=255)
                                                                                  cATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGt                                                        >  2:509171/1‑150 (MQ=255)
                                                                                      ttttTCTCACTACCTCGTCAGTTATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGAGGCCGCTGAGCTGCAAACTGGTTGCCAGGAACTGACAGAGCGGCGCATTGCGGACCGCCAGCCAGCAGCGTTTAc                                                    <  2:282505/150‑1 (MQ=255)
                                                                                      ttttACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTAc                                                    <  2:1003793/150‑1 (MQ=255)
                                                                                      ttttACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGATCCTGCGCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATGGCGGACCGCCAGCCAGCAGCGTTTAc                                                    >  2:109775/1‑150 (MQ=255)
                                                                                          aCTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACt                                                >  1:83800/1‑150 (MQ=255)
                                                                                            tCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCa                                              >  1:665197/1‑150 (MQ=255)
                                                                                             cACTACCTCGTCAGCGAGCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACGGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGCTTACCACTCaa                                             >  2:923595/1‑150 (MQ=255)
                                                                                              aCTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAAc                                            >  2:141905/1‑150 (MQ=255)
                                                                                              aCTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAAc                                            >  1:479331/1‑150 (MQ=255)
                                                                                                tACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAAccc                                          >  3:354479/1‑150 (MQ=255)
                                                                                                   cTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAGACTGGTTTCCAGGAACTGACAGTGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTc                                       >  1:113870/1‑150 (MQ=255)
                                                                                                    tCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCCAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTcc                                      >  2:804634/1‑150 (MQ=255)
                                                                                                    tCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTcc                                      >  2:441998/1‑150 (MQ=255)
                                                                                                      gTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCAc                                    <  1:88539/150‑1 (MQ=255)
                                                                                                      gTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCAc                                    <  2:920277/150‑1 (MQ=255)
                                                                                                       tCAGTGATCAGCACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCAGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGAACGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACg                                   >  4:416028/1‑150 (MQ=255)
                                                                                                        cAGTGATCAACACATCTTCGTGAGTCGGTTCCCGCCCAGGGTATGATGTAACGACTATGCCGCTGCGCTCTACGCTGGTATCCAGGAACTGACAGAGCGACGCATTTCGGACCGCCAGCCAGCAGCGGTTACCACTCTACCCTTCCACGc                                  >  4:238988/1‑150 (MQ=255)
                                                                                                             aTCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCtttt                             >  4:218322/1‑150 (MQ=255)
                                                                                                                aaCACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTc                          >  2:251270/1‑150 (MQ=255)
                                                                                                                aaCACATCTTCGGGAGTCGGTTCCCGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCcagccagc                                                             <  1:607104/115‑1 (MQ=255)
                                                                                                                aaCACATCTTCGGGAGTCGGTTCCCGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCcagccagc                                                             >  2:607104/1‑115 (MQ=255)
                                                                                                                 acacATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTcc                                                                                                     >  1:733522/1‑74 (MQ=255)
                                                                                                                 acacATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTcc                                                                                                     <  2:733522/74‑1 (MQ=255)
                                                                                                                    caTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCg                      <  1:237091/150‑1 (MQ=255)
                                                                                                                     aTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCgg                     <  2:83800/150‑1 (MQ=255)
                                                                                                                     aTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCgg                     <  1:760689/150‑1 (MQ=255)
                                                                                                                       cTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGt                   >  1:1043571/1‑150 (MQ=255)
                                                                                                                        ttCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                   >  2:982174/1‑69 (MQ=255)
                                                                                                                        ttCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                   <  1:982174/69‑1 (MQ=255)
                                                                                                                          cGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACt                <  1:19299/150‑1 (MQ=255)
                                                                                                                          cGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACt                >  2:555685/1‑150 (MQ=255)
                                                                                                                            ggAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGa              >  1:898/1‑150 (MQ=255)
                                                                                                                             gAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAg             >  1:789638/1‑150 (MQ=255)
                                                                                                                                tCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGc                                                                            <  2:625962/84‑1 (MQ=255)
                                                                                                                                tCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGc                                                                            >  1:625962/1‑84 (MQ=255)
                                                                                                                                  ggTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCg        <  1:592488/150‑1 (MQ=255)
                                                                                                                                   gTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGt       >  1:796152/1‑150 (MQ=255)
                                                                                                                                        tGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAAc  <  4:326085/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
ACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAAC  >  NC_000913/2317525‑2317810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑