breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsBOP27_rpe_S2185_L006_R1_001.good.fq1,147,822164,467,569100.0%143.3 bases150 bases91.5%
errorsBOP27_rpe_S2185_L006_R2_001.good.fq1,147,822164,467,569100.0%143.3 bases150 bases90.3%
errorsBOP27_rpe_S2185_L008_R1_001.good.fq505,94372,235,910100.0%142.8 bases150 bases91.4%
errorsBOP27_rpe_S2185_L008_R2_001.good.fq505,94372,235,910100.0%142.8 bases150 bases89.1%
total3,307,530473,406,958100.0%143.1 bases150 bases90.7%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65292.12.0100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 100000100000
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000205
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.014

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.75414

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input14:21:45 28 Feb 202014:22:33 28 Feb 202048 seconds
Read alignment to reference genome14:22:33 28 Feb 202014:29:52 28 Feb 20207 minutes 19 seconds
Preprocessing alignments for candidate junction identification14:29:52 28 Feb 202014:30:39 28 Feb 202047 seconds
Preliminary analysis of coverage distribution14:30:39 28 Feb 202014:32:13 28 Feb 20201 minute 34 seconds
Identifying junction candidates14:32:13 28 Feb 202014:33:21 28 Feb 20201 minute 8 seconds
Re-alignment to junction candidates14:33:21 28 Feb 202014:35:26 28 Feb 20202 minutes 5 seconds
Resolving best read alignments14:35:26 28 Feb 202014:36:36 28 Feb 20201 minute 10 seconds
Creating BAM files14:36:36 28 Feb 202014:38:02 28 Feb 20201 minute 26 seconds
Tabulating error counts14:38:02 28 Feb 202014:38:35 28 Feb 202033 seconds
Re-calibrating base error rates14:38:35 28 Feb 202014:38:37 28 Feb 20202 seconds
Examining read alignment evidence14:38:37 28 Feb 202014:44:56 28 Feb 20206 minutes 19 seconds
Polymorphism statistics14:44:56 28 Feb 202014:44:57 28 Feb 20201 second
Output14:44:57 28 Feb 202014:45:17 28 Feb 202020 seconds
Total 23 minutes 32 seconds