| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,282,927 | 0 | A | G | 20.0% | 58.6 / 10.3 | 30 | I318V (ATC→GTC) | agaS | tagatose‑6‑phosphate ketose/aldose isomerase |
| Reads supporting (aligned to +/- strand): ref base A (12/12); new base G (3/3); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAG > NC_000913/3282822‑3283075 | tcCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTcc < 1:198582/122‑1 (MQ=255)tcCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTcc > 2:198582/1‑122 (MQ=255) ccACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtc < 2:35296/138‑1 (MQ=255) ccACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtc > 1:35296/1‑138 (MQ=255) ccTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGGGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGa < 8:150684/149‑1 (MQ=255) tgatcttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAGGCAGCGACGATGTCGCTGctttc < 3:116178/94‑5 (MQ=38) tgatcttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATAGCAGCGGAAGGCAGCGACGATGTCGCTGcttta > 4:116178/1‑90 (MQ=255) tgAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGcc > 1:34962/1‑148 (MQ=255) ttCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACAtcgtcg < 3:150361/63‑1 (MQ=255) ttCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACAtcgtcg > 4:150361/1‑63 (MQ=255) gCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGtt < 2:24468/110‑1 (MQ=255) gCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGtt > 1:24468/1‑110 (MQ=255) aaCCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGAt < 2:34962/149‑1 (MQ=255) gtAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCcaca > 1:7336/1‑50 (MQ=255) gtAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCcaca < 2:7336/50‑1 (MQ=255) tcgccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa > 7:78740/1‑105 (MQ=255) tcgccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa < 8:78740/105‑1 (MQ=255) gccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCt < 7:24423/137‑1 (MQ=255) gccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCt > 8:24423/1‑137 (MQ=255) gccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCaa > 3:53891/1‑149 (MQ=255) catcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCAttt < 7:128780/91‑1 (MQ=255) catcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCAttt > 8:128780/1‑91 (MQ=255) gcgGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGa > 5:104685/1‑149 (MQ=255) cggcagcGACGATGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGt > 5:123823/3‑66 (MQ=38) cggcagcGACGATGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGt < 6:123823/64‑1 (MQ=38) gcagcGACGATGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa > 5:146295/1‑87 (MQ=255) gcagcGACGATGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa < 6:146295/87‑1 (MQ=255) cagcGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa > 2:136948/1‑86 (MQ=255) cagcGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa < 1:136948/86‑1 (MQ=255) cATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCa < 6:104685/149‑1 (MQ=255) aTCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAg > 3:30189/1‑149 (MQ=255) | TCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAG > NC_000913/3282822‑3283075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |