New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 408127339 (1.060)6 (0.180) 6/224 2.8 13.2% coding (588/636 nt) fdoI formate dehydrogenase‑O, cytochrome b556 subunit
?NC_000913 = 4081294 43 (1.260)coding (567/636 nt) fdoI formate dehydrogenase‑O, cytochrome b556 subunit
Rejected: Frequency below/above cutoff threshold.

CGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4081117‑4081273
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑taccagcgcCTGGGCGAAGAAACATCACCCGCGCTGGTACCGTGAGGTCCGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATT  <  NC_000913/4081294‑4081152
                                                                                                                                                                                                                                                                                                            
CGCGTCTGTTGTATAATTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGT                                                                                                                                                         <  4:116521/149‑1
    GCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCA                                                                                                                                                     <  5:58719/149‑1
                 TTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTAC                                                                                                                                                       <  6:28538/134‑1
                 TTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTAC                                                                                                                                                       >  5:28538/1‑134
                                                  ATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCCTGGGCGAAGAAACATCACCCGCGCTGGTACCGTGAG                                                                                                            >  8:136766/1‑144
                                                  ATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCCTGGGCGAAGAAACATCACCCGCGCTGGTACCGTGAG                                                                                                            <  7:136766/144‑1
                                                        GCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTA                                                                                                                                                        <  1:179068/94‑1
                                                        GCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTA                                                                                                                                                        >  2:179068/1‑94
                                                            TACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCCTGGGCGAAGAAACATCACCCGCGCTGGTACC                                                                                                                 >  5:75455/1‑129
                                                            TACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCCTGGGCGAAGAAACATCACCCGCGCTGGTACC                                                                                                                 <  6:75455/129‑1
                                                             ACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCCTGGGCGAAGAAACATCACCCGCGCTGG                                                                                                                     >  2:38364/1‑124
                                                             ACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCCTGGGCGAAGAAACATCACCCGCGCTGG                                                                                                                     <  1:38364/124‑1
                                                                                                      GAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTAC                                                                                                                                                       >  5:66430/1‑49
                                                                                                      GAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTAC                                                                                                                                                       <  6:66430/49‑1
                                                                                                                                                      CCAGCGCCTGGGCGAAGAAACATCACCCGCGCTGGTACCGTGAGGTCCGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTAT   >  7:116056/1‑149
                                                                                                                                                       CAGCGCCTGGGCGAAGAAACATCACCCGCGCTGGTACCGTGAGTTCCGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATT  <  6:176488/149‑1
                                                                                                                                                       CAGCGCCTGGGCGAAGAAACATCACCCGCGCTGGTACCGTGAGGTCCGCAA                                                                                                    >  2:10848/1‑51
                                                                                                                                                       CAGCGCCTGGGCGAAGAAACATCACCCGCGCTGGTACCGTGAGGTCCGCAA                                                                                                    <  1:10848/51‑1
                                                                                                                                                                                                                                                                                                            
CGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4081117‑4081273
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑taccagcgcCTGGGCGAAGAAACATCACCCGCGCTGGTACCGTGAGGTCCGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATT  <  NC_000913/4081294‑4081152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.