New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3262180 =85 (0.880)4 (0.040) 4/252 11.4 4.8% coding (239/2295 nt) tdcE pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
?NC_000913 3262220 = 80 (0.890)coding (199/2295 nt) tdcE pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTAATGTCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGCGACGCCTGCCACCACGGAATTGTGGGAAAAAGTAATGGAAGGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3262336‑3262180
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tttcgatacGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTCCAGTCCGTACCTTTAAAG  >  NC_000913/3262220‑3262365
                                                                                                                                                                                                                                                                                                               
aTAATGGCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGAGATGAATCTTTCCTCGCCGAAGCGTCGCCTGCCACCACGGAATTGTGGGAAAAAGTAATGGAAGGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGAT                                                                                                                                                            <  4:315613/148‑1
TTAATGTCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGCGACGCCTGCCACCACGGAATTGTGGGAAAAAGTAATGGAAGGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGAT                                                                                                                                                            <  8:360777/149‑1
      TCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGCGACGCCTGCCACCACGGAATTGTGGGAAAAAGTAATGGAAGGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGA                                                                                                                                                       <  5:323439/148‑1
        CGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGCGACGCCTGCCACCACGGAATTGTGGGAAAAAGTAATGGAAGGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGA                                                                                                                                                       >  5:125279/1‑146
        CGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGCGACGCCTGCCACCACGGAATTGTGGGAAAAAGTAATGGAAGGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGA                                                                                                                                                       <  6:125279/146‑1
                              ATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGCGACGCCTGCCACCACGGAATTGTGGGAAAAAGTAATGGAAGGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGAT                                                                                                                                                      <  7:325195/125‑1
                              ATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGCGACGCCTGCCACCACGGAATTGTGGGAAAAAGTAATGGAAGGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGAT                                                                                                                                                      >  8:325195/1‑125
                                                                                                              GGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATACGGATGCCGTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTC                                                                        >  5:217350/1‑123
                                                                                                              GGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATACGGATGCCCTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTC                                                                        <  6:217350/123‑1
                                                                                                                  TCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATA                                                            >  3:134068/1‑131
                                                                                                                  TCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATA                                                            <  4:134068/131‑1
                                                                                                                                                     TTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTT                      >  3:254395/1‑134
                                                                                                                                                     TTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTT                      <  4:254395/134‑1
                                                                                                                                                       CGATACGTATGCCTTCCATTACTTTTTCCCACAATTCCGTTGTGGCAGGCGTCGCTTCGGCGAGTAAAGATTCATCGCCTTCATACGGTGTATAGGTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTCCAGTCCGTACCTTT      <  5:10961/148‑1
                                                                                                                                                        GATACGGATGCCCTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTCCAGTCCGTACCTTTA     <  6:408848/148‑1
                                                                                                                                                          TACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTCCAGTCCGTACCTTTAAAG  >  6:402014/1‑149
                                                                                                                                                                                                                                                                                                               
TTAATGTCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGCGACGCCTGCCACCACGGAATTGTGGGAAAAAGTAATGGAAGGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3262336‑3262180
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tttcgatacGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTCCAGTCCGTACCTTTAAAG  >  NC_000913/3262220‑3262365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.