| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,512,350 | 1 | . | G | 21.7% | 58.0 / 19.1 | 23 | coding (65/957 nt) | fecD | ferric citrate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base . (9/9); new base G (2/3); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGC > NC_000913/4512212‑4512497 | gccACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACT‑CCATATGGGAGTGAtcc < 1:118830/149‑3 (MQ=255) aCAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAggg > 2:140069/1‑148 (MQ=255) aCAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAgg < 5:181024/149‑1 (MQ=255) cagcagCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGca < 3:97997/124‑1 (MQ=255) cagcagCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGca > 4:97997/1‑124 (MQ=255) agcagcAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGtgatga > 5:15793/1‑148 (MQ=255) agcaAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAAt < 6:15793/149‑1 (MQ=255) cATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTc < 5:83296/148‑1 (MQ=255) aTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGAt < 8:137593/83‑1 (MQ=255) aTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGAt > 7:137593/1‑83 (MQ=255) tCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAAcc < 4:159727/111‑1 (MQ=255) tCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAAcc > 3:159727/1‑111 (MQ=255) gtCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAg > 3:88560/1‑148 (MQ=255) tCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCa < 4:88560/149‑1 (MQ=255) cAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGcc > 7:134682/1‑148 (MQ=255) gTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACT‑CCATATGGGAGTGAtc > 7:10879/1‑52 (MQ=25) gTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACT‑CCATATGGGAGTGAtc < 8:10879/53‑2 (MQ=25) cGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCa < 1:140069/149‑1 (MQ=255) gctgttaTCACT‑CCATATGGGAGTGATCACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCg > 4:100366/7‑146 (MQ=255) gctgttaTCACT‑CCATATGGGAGTGATCACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCg < 3:100366/140‑1 (MQ=255) ggATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCggg > 4:141106/1‑144 (MQ=255) cTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCgg < 8:134682/149‑1 (MQ=255) tCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCggg > 6:156556/1‑149 (MQ=255) t‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTTCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGc > 4:142818/1‑148 (MQ=255) | GCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATAT‑GGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGC > NC_000913/4512212‑4512497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |