Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,7360CA43.2% 17.4 / 56.0 44A277E (GCG→GAG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (12/13);  new base A (10/9);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTC  >  NC_000913/756607‑756875
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aGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAATCATGGCGACCGTTTTAGGGAGCGTTATGCGCCGAACGCCAAAGACCTg                                                                                                                          >  3:15593/1‑149 (MQ=255)
  gATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGc                                                                                                                        >  7:10246/1‑149 (MQ=255)
  gATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCTGGc                                                                                                                        >  8:139907/1‑149 (MQ=255)
           aTGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCt                                                                                                                           >  8:152473/1‑137 (MQ=255)
           aTGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTCGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCt                                                                                                                           <  7:152473/137‑1 (MQ=255)
              tgGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGt                                                                                                            <  1:187766/149‑1 (MQ=255)
              tgGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGATGAACAAACATGGCGAACGTTGTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGt                                                                                                            >  8:69922/1‑149 (MQ=255)
                        aCCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGt                                                                                                  >  7:142336/1‑149 (MQ=255)
                             aCCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGcc                                                                                                                   <  6:20110/127‑1 (MQ=255)
                                ggCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCcatcat                                                                                          <  8:142336/149‑1 (MQ=255)
                                                gCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGaa                                                                          <  8:10246/149‑1 (MQ=255)
                                                   tACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGt                                                                       >  5:92904/1‑149 (MQ=255)
                                                   tACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGt                                                                       <  7:139907/149‑1 (MQ=255)
                                                      tGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTcgc                                                                    >  3:141100/1‑149 (MQ=255)
                                                           aCCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTgg                                                                                                                         >  2:82475/1‑91 (MQ=255)
                                                           aCCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTgg                                                                                                                         <  1:82475/91‑1 (MQ=255)
                                                           aCCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCtg                                                               >  6:51470/1‑149 (MQ=255)
                                                           aCCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCtg                                                               >  4:85671/1‑149 (MQ=255)
                                                           aCCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGCCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCtg                                                               >  7:107148/1‑149 (MQ=255)
                                                              gAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGa                                                            <  5:51470/149‑1 (MQ=255)
                                                                  gTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATcc                                                                               >  2:43552/1‑126 (MQ=255)
                                                                  gTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATcc                                                                               <  1:43552/126‑1 (MQ=255)
                                                                        gtggtgAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGTGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTgg                                                  >  4:63158/1‑149 (MQ=255)
                                                                          ggtgAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTgggg                                                <  7:69922/149‑1 (MQ=255)
                                                                           gtgAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGTCCTGGCGGGCCGTGACGTGGTTGCGCGGTCCATCATGATCGAAATCCTTGAAGGTCGCGGCTGTTATGGTCCGTgggg                                                <  4:15593/148‑1 (MQ=255)
                                                                                     ttATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACAcgcg                                      >  3:56978/1‑148 (MQ=255)
                                                                                        tCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaa                                   <  4:56978/148‑1 (MQ=255)
                                                                                        tCTGCTGAACAAAAATGGCGAACGTTTTATTGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaa                                   <  8:107148/148‑1 (MQ=255)
                                                                                           gctgAACAAACATGGCGAACGTTTTATGGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaactg                                >  2:14965/1‑148 (MQ=255)
                                                                                               aacaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGt                                                    >  4:146611/1‑124 (MQ=255)
                                                                                               aacaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGt                                                    <  3:146611/124‑1 (MQ=255)
                                                                                                 caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCg                         <  3:85671/149‑1 (MQ=255)
                                                                                                    acaTGGCGAACGTTTTATGGAGCGTTATGAGCTGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGAt                       >  6:2523/1‑148 (MQ=255)
                                                                                                    acaTGGCGAACGTTTTATGGAGCGTTATGAGCTGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATc                      <  5:2523/149‑1 (MQ=255)
                                                                                                      aTGGCGAACGTTTTATGGAGCGTTATGCGACGAACTCCAAAGACCTGGCGGGACGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTAGATCAc                    >  5:175354/1‑149 (MQ=255)
                                                                                                         gCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGTGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCt                  <  3:63158/148‑1 (MQ=255)
                                                                                                               gTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGt                                                        <  4:14232/104‑1 (MQ=255)
                                                                                                               gTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGt                                                        >  3:14232/1‑104 (MQ=255)
                                                                                                                     tGGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATc                      >  2:131732/1‑132 (MQ=255)
                                                                                                                     tGGAGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATc                      <  1:131732/132‑1 (MQ=255)
                                                                                                                        aGCGTTATGCGCCGAACGCCAAAGACCTGGCTGGCCGTGACGTGGTTTCTCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTTATGGTCCGTGGTGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtctc  <  6:175354/149‑1 (MQ=255)
                                                                                                                        aGCGTTATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtctc  <  4:141100/149‑1 (MQ=255)
                                                                                                                             tATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaactga                               >  1:8884/1‑115 (MQ=255)
                                                                                                                             tATGAGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaactga                               <  2:8884/115‑1 (MQ=255)
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AGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTC  >  NC_000913/756607‑756875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑