| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 774,637 | 0 | C | G | 20.8% | 53.5 / 10.7 | 24 | intergenic (+35/‑115) | ybgE/ybgC | putative inner membrane protein in cydABX‑ybgE operon/acyl‑CoA thioester hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (10/9); new base G (2/3); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTGATTCACGGCGTGGGCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCG > NC_000913/774491‑774785 | tgtgATTCACGGCGTGGGCTTTTGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAg > 6:61002/1‑149 (MQ=255) gggCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCTGGAGCGGCCCATAATTTTTTa < 2:54671/149‑1 (MQ=39) ggcgtgcGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTGTGCCCATTCCTCGCCGATATTGCTCTCATTGTCGGGCTGATTTTCATCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCTGGAGCGGCCCATAATTTTTTaa < 5:45089/143‑2 (MQ=37) cTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAATTTATGGGCCTGGAGCGGCCCATAATTTTTTa > 1:165719/1‑146 (MQ=38) cTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAATTTATGGGCCTGGAGCGGCCCATAATTTTTTa > 1:168351/1‑146 (MQ=38) cGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATCTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAATTTATGGGCCTGGAGCGGCCCATAATTTTTTaaagagaa < 1:115132/149‑8 (MQ=37) cgcgGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTaa > 8:145002/1‑149 (MQ=255) cggAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACt < 7:145002/147‑1 (MQ=255) cAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACt > 1:152800/1‑149 (MQ=255) gTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGccc < 2:152800/148‑1 (MQ=255) ggATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGccc < 2:102968/135‑1 (MQ=255) ggATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGccc > 1:102968/1‑135 (MQ=255) ttGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATcg < 5:61002/148‑1 (MQ=255) gcCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTAtagtag > 7:134882/1‑149 (MQ=255) ccGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGta > 2:12986/1‑142 (MQ=255) ccGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGta < 1:12986/142‑1 (MQ=255) ttCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTa > 4:71417/1‑148 (MQ=255) tCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTaa < 3:71417/149‑1 (MQ=255) ttttCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTttgttg > 5:104403/1‑149 (MQ=255) tcttcttTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAGCTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTAtagta > 4:8554/1‑114 (MQ=255) aTCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTCTTGCATTACCGGGATGTaaa < 8:134882/149‑1 (MQ=255) ttCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATa < 6:104403/149‑1 (MQ=255) tctcTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACtttt > 8:15144/1‑120 (MQ=255) tctcTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACtttt < 7:15144/120‑1 (MQ=255) cAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTcgcg > 1:114802/1‑149 (MQ=255) | TGTGATTCACGGCGTGGGCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCG > NC_000913/774491‑774785 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |