| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 380,013 | 0 | G | . | 26.5% | 82.9 / 28.9 | 35 | intergenic (‑132/+56) | frmR/yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (23/2); new base . (6/3); total (30/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC > NC_000913/379867‑380153 | cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGACATTTACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACGGGAGACTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑gg < 2:37130/148‑1 (MQ=255) tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGc > 3:56476/1‑149 (MQ=255) ggCCTTTCGCACCTCATCATATGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGATAAGACAGTTTTTCCGGTGATTGTGGTCGGAAGACAATGAGGAAA‑TGGGGGGTGGCTa > 6:421265/1‑149 (MQ=255) ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGAAA‑gggggggggggga > 6:43641/1‑146 (MQ=255) cATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATc > 5:215346/1‑149 (MQ=255) aTTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCg > 1:458255/1‑149 (MQ=255) tttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGATAATCg > 5:8949/1‑149 (MQ=255) tttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCAGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGATAAACg > 3:327758/1‑149 (MQ=255) cccACCTCATCATATTCATGAAATATACTATTGGGGGGTATGATATATGTCAATGCATCCCCCCCTATATTAAATAACACTTCCAAATAAATTTTAACCGGTGAATTGGGGCTTAACACCAAGCGGAAA‑‑GGGGGGGGGCTAATCGGCa < 1:302650/146‑1 (MQ=255) ccTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTAAAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGACGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAggg > 6:79305/1‑147 (MQ=255) atcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAGGGAAGgccgcc > 2:406438/1‑149 (MQ=255) tcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGGTATTGGGCTGGGAAGgccccc > 7:374042/1‑149 (MQ=255) cTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCGCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGGAAACAAAGAGGAAT‑GGGGGGGGGGCTAAGCGGAAGGGAAGgc > 5:78312/1‑144 (MQ=255) catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGATAAGCGGCAGGGAAGGCCGCCCCg > 6:151231/1‑149 (MQ=255) gcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTCAATGTGGTCGGAAAACAAAGAGGAAA‑GGTGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa > 6:158617/1‑147 (MQ=255) cggtggTATACCATATCTCAATGCACACCCCCCTATAGAAAAATACACTCGCGAAAAAAAATTCTCCGGGCCACGGCGGCCCAAAACAAACCCCCAA‑GGGGCGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc < 8:86215/141‑1 (MQ=255) tATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGGTAATAGGCAGGGCAGGCCGCCCCGGAAAGGGGGCGGCCGAAGGGAt > 5:220310/1‑148 (MQ=255) tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa > 1:294156/1‑139 (MQ=255) tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg < 2:458255/148‑1 (MQ=255) gcAATGCCTACCCCCCTATAGGATAAACCACTGGCGCATAAAAATTATCCGGGGAAAGTGGTCGGAAAACAAAGCGGAAC‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa < 2:294156/138‑1 (MQ=255) aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt > 7:359959/1‑148 (MQ=255) aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAAACGGCAGGGAAGGCCCCCCCGGATACCGGGCGGCAGAAGGAATAAGAATTTCCAGGt > 7:88164/1‑148 (MQ=255) taGTATTTAATAATGTAGAATAAAATTTATACGGTCAATGTGTTAGTAAAACAAATATGAAA‑‑GGGTGGGTGCTAATCGTCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt < 4:269715/149‑1 (MQ=255) aGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGGTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAGCAGAATTTCCAGGTCAGACGGGCTTCAAGtt > 7:195150/1‑149 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGAGAAAACGGCAGGGAGGGCCCCCACGGATTCCGGCGGGCAGAAGGAAAAGGATTTACCGGGTCTGCCGGGCTGCACGTTCCAGccc > 5:407558/1‑149 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGATTAGGGGCGGGGAGGGCCGCCCCGGTTGGCGGGCGGGGGCTGGATGCGGCTTTGCCGGGGCAGACGGGCGGCAAGTGGCGGACCGtt > 6:376153/1‑148 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAAGCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGATGCAGAATTTCCAGGTCACACGGGCTGCAAGTGGCAGACCGTTaa > 8:311555/1‑148 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAat > 7:172348/1‑149 (MQ=255) aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg > 4:408733/1‑149 (MQ=255) tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTGCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgt > 3:13535/1‑149 (MQ=255) gTGAATGAGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCCTAAACGCCAGGGAAGGCCGCCCCGAATAGCGGCAGGCCGAAGAAATCAAAATTTCAAGGTCAAACTGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTGTGGGTTTcc > 8:166475/1‑148 (MQ=255) ggTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCacac > 6:314468/1‑149 (MQ=255) tCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGACGGAATCAGAATTTCCAGGTCAGCCGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCCCACtt > 7:460965/1‑149 (MQ=255) gAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAGCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc > 4:18115/1‑149 (MQ=255) cAAAGAGGAAA‑GGGGGGGGCGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCAAGGTCAGACGGGCTCCAAGTTGCAGACCGTTAAAATCATCGGTAGGGGTGTCGAACCACACTTTACCTGcc > 7:80906/1‑149 (MQ=255) aaGAGGAAA‑GGGGGGGGGGCTTATCGGGAGGGAAGGCCGCCCCGGGTAGGGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGGCGGGCTGCAAGTTGCAGGCCGTTAATATGATGGGTTGGGGTGTCGTTCCCCCCTTTACCTGCCgg > 2:300360/1‑148 (MQ=255) agagGAAA‑GGGGGGTGGGCTAATAGGCAGGGAAGGCCACCCCGGAAAGCGGGCGGAAGAAGTAATAAGAATTTCCAGGTCAGACGGGCTGCAAGGTGCAGACAGTTAAAAGAATCGGTTGGGGGGTCGTAACAAAATTTACCTGCCGTc > 2:195801/1‑149 (MQ=255) | CGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC > NC_000913/379867‑380153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |