Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913763,8950CG30.3% 50.9 / 26.3 33T294T (ACC→ACG) sucCsuccinyl‑CoA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (12/11);  new base G (6/4);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGAACTGAACTACGTTGCGCTGGACGGTAACATCGGTTGTATGGTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTAC  >  NC_000913/763757‑764043
                                                                                                                                          |                                                                                                                                                    
ggAACTGAACTACGTTGCGCTGGACGGTAACATCGGTTGTATGGTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACgtg                                                                                                                                            >  6:74343/1‑149 (MQ=255)
       aactACGTTGCGCTGGACGGTAACATCGGTTGTATGGTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACGAAAGAACGTGTAACCg                                                                                                                                      >  8:60490/1‑148 (MQ=255)
       aactACGTTGCGCTGGACGGTAACATCGGTTGTATGGTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCg                                                                                                                                      >  2:84240/1‑148 (MQ=255)
       aactACGTTGCGCTGGACGGTAACATCGGTTGTATGGTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGATAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCg                                                                                                                                      >  4:5019/1‑148 (MQ=255)
                  gcTGGACGGTAACATCGGTTGTATGGTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCaaaa                                                                                                                          <  2:21624/149‑1 (MQ=255)
                       aCGGTAACATCGGTTGTATGGTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAatcatc                                                                                                                     <  1:84240/149‑1 (MQ=255)
                               aTCGGTTGTATGGTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTga                                                                                                             <  3:5019/149‑1 (MQ=255)
                                         tGGTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGt                                                                                                    <  5:74343/148‑1 (MQ=255)
                                          ggTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTg                                                                                                   >  1:30921/1‑148 (MQ=255)
                                            ttAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGa                                                                                                  >  4:54873/1‑147 (MQ=255)
                                              aaCGGCGCATGTCTGGAGATGGGTACGATGGACATCTTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACGAAAGAACGTCTAACCGAAGCGTTCAAAATCATCCTCTCTGACAAAAAAGTg                                                                                                   >  6:18024/1‑144 (MQ=255)
                                              aaCGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACTGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACGAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTg                                                                                                   <  5:18024/144‑1 (MQ=255)
                                              aaCGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGc                                                                                              >  7:135663/1‑149 (MQ=255)
                                                 ggCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGCACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGt                                                                                           >  5:51428/1‑149 (MQ=255)
                                                                 tGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACGAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTc                                                                           <  7:60490/149‑1 (MQ=255)
                                                                        gATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACGAAAGa                                                                                                                                                 <  6:38044/72‑1 (MQ=255)
                                                                        gATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACGAAAGa                                                                                                                                                 >  5:38044/1‑72 (MQ=255)
                                                                              cATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAtcgttc                                                              <  8:135663/149‑1 (MQ=255)
                                                                               aTCGTTAAACTGCACGGCGTCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACgt                                                                                                                                             <  5:64410/69‑1 (MQ=255)
                                                                               aTCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCATGACGTTGGCGGCGCCGCAACCAAAGAACgt                                                                                                                                             >  6:64410/1‑69 (MQ=255)
                                                                                tCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACGAAAGAACGTGTAACCGAAGCGTTCAAAatc                                                                                                                        >  5:145413/1‑89 (MQ=255)
                                                                                tCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACGAAAGAACGTGTAACCGAAGCGTTCAAAatc                                                                                                                        <  6:145413/89‑1 (MQ=255)
                                                                                         tGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCt                                                    >  6:76571/1‑148 (MQ=255)
                                                                                              ggcggcGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGc                                              <  5:76571/149‑1 (MQ=255)
                                                                                                             aaCTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACaaa                                                                                                      <  1:160130/78‑1 (MQ=255)
                                                                                                             aaCTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACaaa                                                                                                      >  2:160130/1‑78 (MQ=255)
                                                                                                              aCTTCCTTGACGTTGGCGGCGGCGCAACGAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGgc                              >  5:111738/1‑149 (MQ=255)
                                                                                                                ttCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTcgg                                                                         >  1:71937/1‑104 (MQ=255)
                                                                                                                ttCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTcgg                                                                         <  2:71937/104‑1 (MQ=255)
                                                                                                                            ggcggcggcGCAACGAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGt                >  4:22247/1‑149 (MQ=255)
                                                                                                                              cggcggcgCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTgg              <  2:30921/149‑1 (MQ=255)
                                                                                                                               ggcggcgCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTggg             <  6:51428/149‑1 (MQ=255)
                                                                                                                                        aCCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACg     >  2:167674/1‑148 (MQ=255)
                                                                                                                                         ccAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACaaa                                                                                                      <  4:124457/50‑1 (MQ=255)
                                                                                                                                         ccAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACaaa                                                                                                      >  3:124457/1‑50 (MQ=255)
                                                                                                                                          cAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTAc  >  3:73475/1‑149 (MQ=255)
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GGAACTGAACTACGTTGCGCTGGACGGTAACATCGGTTGTATGGTTAACGGCGCAGGTCTGGCGATGGGTACGATGGACATCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTAC  >  NC_000913/763757‑764043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑