New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2645510 =	24 (0.970)	4 (0.170)	3/260	3.0	14.5%	coding (1816/2313 nt)	pbpC	penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
	?	NC_000913	2645550 =	24 (1.000)					coding (1776/2313 nt)	pbpC	penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTTGCCATTTTCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGAC  >  NC_000913/2645409‑2645689                                                                                                                                              |                                                                                                                                            tttGCCATTTTCATCCAGCCAGATGGGGAAACGACTGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGTCTGCGGCGACAGTTACCGTCCCCTTACGGCAgagaggaag                                                                                                                                      >  1:15/1‑144 (MQ=255)           tCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGcc                                                                                                                             <  4:107050/148‑1 (MQ=255)             aTCCAGCCAGATGGGGAAACGCATGCCATTAATGCCTTCCTGCTCCGGCAGTAAGAGAGTAGGCGGCTGACTCCCGTCCCTCCGCCAGGTTGCCCGGCGGCGGCGCCAGGTACCGTCACCTTCCGGCAACGACTGACCTCCCGGCcag                                                                                                                           >  1:106469/1‑148 (MQ=255)                cagccagATGGGGAAACGAATGCCATTAATGCCTTCCTGGTCCGGCAGTAATAGAGTTGGCGGGTGACTCCCTTCCAGCAGCCAGGTTGCCAGGCGGCGGCGTCAGTTACCGTCACCTTCCGTCAAAGACTGTCCGCCCTGCCAGCAGa                                                                                                                       <  1:14958/149‑1 (MQ=255)                      gATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGc                                                                                                                 >  5:21195/1‑149 (MQ=255)                           ggAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGa                                                                                                            <  6:21195/149‑1 (MQ=255)                            gAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGc                                                                                                                              >  2:140639/1‑130 (MQ=255)                                       cATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTc                                                                                                <  3:10884/149‑1 (MQ=255)                                       cATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTc                                                                                                >  2:93892/1‑149 (MQ=255)                                                 ttCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGt                                                                                      >  6:116023/1‑149 (MQ=255)                                                    cTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCtt                                                                                   >  2:72878/1‑149 (MQ=255)                                                     tGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTc                                                                                  >  4:107592/1‑149 (MQ=255)                                                           ggCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGAt                                                                                                                      >  2:38691/1‑107 (MQ=255)                                                           ggCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGAt                                                                                                                      <  1:38691/107‑1 (MQ=255)                                                           ggCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCgg                                                                               >  4:3736/1‑146 (MQ=255)                                                                tAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCg                                                                       <  3:3736/149‑1 (MQ=255)                                                                            cggcTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACa                                                           <  1:72878/149‑1 (MQ=255)                                                                                                 ccAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGAt                                      >  3:18536/1‑149 (MQ=255)                                                                                                   aGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGAtt                                     >  6:139830/1‑148 (MQ=255)                                                                                                     gTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTcaa                                  <  3:107592/149‑1 (MQ=255)                                                                                                          cAGGCGTCGGCGACAGTTACCGGCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGAGAACGCCACGAGTGACTGCGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGAGCTGATTCAACAATg                             <  2:106469/149‑1 (MQ=255)                                                                                                            ggcggcggcGACAGTTACCGTCTCCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGt                           <  3:125878/149‑1 (MQ=255)                                                                                                                                     ttCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGAc  >  6:18504/1‑149 (MQ=255)                                                                                                                                       ccGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGAc  <  1:93892/147‑1 (MQ=255)                                                                                                                                          gCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTcaacaa                               >  5:25920/1‑115 (MQ=255)                                                                                                                                          gCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTcaacaa                               <  6:25920/115‑1 (MQ=255)                                                                                                                                              |                                                                                                                                            TTTGCCATTTTCATCCAGCCAGATGGGGAAACGAATGCCATTAATGCCTTCCTGCTCCGGCAGTAATAGAGTTGGCGGCTGACTCCCGTCCAGCAGCCAGGTTGCCAGGCGGCGGCGACAGTTACCGTCACCTTCCGGCAAAGACTGTCCGCCCGGCCAGCAGATAACGCCACGAGTGACTGAGTTCGGGCGCGGGTCTTCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGAC  >  NC_000913/2645409‑2645689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.