Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,284,630	G→A	L85L (CTG→CTA)	yejM →	essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,284,630	0	G	A	100.0%	89.4 / NA	28	L85L (CTG→CTA)	yejM	essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

CTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTGATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGC  >  NC_000913/2284487‑2284770                                                                                                                                                |                                                                                                                                             cTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAg                                                                                                                                         <  3:28214/149‑1 (MQ=255)                  gCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTc                                                                                                                        >  2:145353/1‑149 (MQ=255)                           gCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAAcgg                                                                                                               >  6:128943/1‑149 (MQ=255)                                 tGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGaa                                                                                                         >  4:80535/1‑149 (MQ=255)                                                   cGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATc                                                                                        <  3:80535/148‑1 (MQ=255)                                                    gTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCg                                                                                       >  1:15963/1‑148 (MQ=255)                                                         cATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGc                                                                                  >  5:120932/1‑148 (MQ=255)                                                           ttctCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGaa                                                                               <  6:86687/146‑1 (MQ=255)                                                                ggCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCtt                                                                          <  5:128943/149‑1 (MQ=255)                                                                  ccATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTa                                                                        <  2:24702/149‑1 (MQ=255)                                                                              ccTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTtcca                                                              <  1:145353/147‑1 (MQ=255)                                                                                  gtgtTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCg                                                                                 <  2:26374/124‑1 (MQ=255)                                                                                  gtgtTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCg                                                                                 >  1:26374/1‑124 (MQ=255)                                                                                            ccTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATcc                                               >  3:70309/1‑148 (MQ=255)                                                                                                cTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATc                                           <  2:15963/148‑1 (MQ=255)                                                                                                   gCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTc                                        <  6:120932/148‑1 (MQ=255)                                                                                                    cTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTccatctccatct                                                     >  2:82276/1‑134 (MQ=255)                                                                                                    cTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTccatctccatct                                                     <  1:82276/134‑1 (MQ=255)                                                                                                       aTCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCGATTACTGATCGATTGAGAAGTCTTTACTAGTTTCCATCTCCATAATAATCCCATAGTCTGGc                                    >  6:77247/1‑148 (MQ=255)                                                                                                               cccGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGtt                            <  5:77247/148‑1 (MQ=255)                                                                                                                 cGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCtt                                                                          >  5:91415/1‑100 (MQ=255)                                                                                                                 cGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCtt                                                                          <  6:91415/100‑1 (MQ=255)                                                                                                                      aCCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTg                               >  6:135191/1‑138 (MQ=255)                                                                                                                      aCCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGAGCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTg                               <  5:135191/138‑1 (MQ=255)                                                                                                                        cTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCaa                       <  5:82982/144‑1 (MQ=255)                                                                                                                        cTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCaa                       >  6:82982/1‑144 (MQ=255)                                                                                                                        cTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAAcc                     >  1:51059/1‑146 (MQ=255)                                                                                                                                        cAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCATAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGgc  >  5:97084/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                             CTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTGATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGC  >  NC_000913/2284487‑2284770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑