Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0130G.27.3% 48.6 / 17.0 25intergenic (‑132/+56)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (15/1);  new base . (3/3);  total (18/7)
Fisher's exact test for biased strand distribution p-value = 4.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC  >  NC_000913/379867‑380153
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cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACATAGAGGAAAggg                                                                                                                                            >  1:299628/1‑149 (MQ=255)
        tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGTTGAATGTGGTCGGAAAACAAAGAGGAAAgtggggggggg                                                                                                                                    >  5:191308/1‑148 (MQ=255)
                   cACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTAACCGTTGAATGTGGTCGGAAAACAAATAGGAAAGGGGGGGGGGGTATTCGGCTgg                                                                                                                         >  7:343922/1‑149 (MQ=255)
                    aCCTCATCATCTGCATGCAATATACTATAGGGTGGTATTCTATATGTCAATGCATACCCCCATATAGTATATAACACTGTATAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAg                                                                                                                          <  5:36775/146‑1 (MQ=255)
                    aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGTAATCGGCAgg                                                                                                                         >  7:256906/1‑148 (MQ=255)
                    aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACT‑GAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGATATACGGAAggg                                                                                                                        >  8:28302/1‑148 (MQ=255)
                        catcatCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgc                                                                                                                   >  2:188298/1‑149 (MQ=255)
                         atcatcTGCATTCAATATACTTTCGGGGGTTATTCTATATGTCAATTCATACCCCCCTATAGTATATAACACTGGATAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc                                                                                                                  <  2:77631/149‑1 (MQ=255)
                             tcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCGTATGGGCAGGGACGgccgcc                                                                                                               >  8:367577/1‑149 (MQ=255)
                                   agcaATATACTATAGGGGCGTATTCTATATGTCAAGGCATACCCCCCAATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑AACGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                        <  3:251181/148‑1 (MQ=255)
                                     caATTTACTATAGGGGGGTCTTCTATTTTTCAACCCATCCCCCCCTACTTTATAAAACACGGGCGCATAACATTTACCCCGGCCCCGGGGGCGGACACCCACCCGGACCGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                        <  2:345921/148‑1 (MQ=255)
                                        tataCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                                                                                           >  1:287916/1‑110 (MQ=255)
                                        tataCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                                                                                           <  2:287916/110‑1 (MQ=255)
                                                         ttCTATATGTCAATGCATACCCCCCTAAAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTTGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATACCGCGCGGCAGAAGGaaa                                                                                      >  6:194987/1‑145 (MQ=255)
                                                           cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGTGGGGGGCTATGCGGAAGGGATGCCCGCCCCGAATAGCGGGCGGCAGAATGaaaaagat                                                                                 >  8:83760/1‑143 (MQ=255)
                                                            tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                                                                >  6:236225/1‑149 (MQ=255)
                                                              tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                                                             >  4:311626/1‑149 (MQ=255)
                                                                tGTCACAGCATACCCCCCTAAAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGGCGGAAAACAAAGAGGAAA‑GGGGGGCGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                                                           <  7:166761/149‑1 (MQ=255)
                                                                                         tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAAAAAAGAGGAAAGGGGGGGGGGAAAATCGGGAGGGAACGCCGCCCCGGATAGCGGTCGCCAGAAGGAAGCAGAATTTCCAGGTCACACGGGCTGCAACTTGCAg                                                   >  5:357546/1‑149 (MQ=255)
                                                                                          ataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                                                 >  3:247410/1‑149 (MQ=255)
                                                                                           taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGTTTGCGGGCGGCTGCAGGAATCCGAATTGCCAGGTCCGACGGGCTGAAAGTTGCAgcc                                                 >  2:236939/1‑147 (MQ=255)
                                                                                                     gaATAAAATTTATCCGGTGAATGTGGTCGGTAAACAAAGAGGAAAGGGGGGGGGGATAATCGGCTGGGAAGGCCGCCCCGGATAGGGGGGGGCAGAGGGAAGCAGACTTTCGAGGGCAGCCGGGCTGCAGGTGGCTGCCCGGTaaatt                                        >  6:305855/1‑146 (MQ=255)
                                                                                                        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCCGACCGTTAAAatcatc                                    >  7:92889/1‑149 (MQ=255)
                                                                                                        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGCGGGGCTAATCGGCAGGGAAGGCCGCCCCGCATAGCGGGCGGAAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAATTTGCAGACCGTTAAAatcatc                                    >  5:338217/1‑149 (MQ=255)
                                                                                                          aaaTCTTTCCGGGGCATGTGGGCCGAAACCAACGAGG‑CACGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                                 <  4:247410/149‑1 (MQ=255)
                                                                                                           aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCCCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                                 >  7:124859/1‑149 (MQ=255)
                                                                                                               tATCCGGTGAATGGGGTCGGAAAACAAACACG‑AACGGGGGGCGCCTAAGCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg                             <  1:188298/148‑1 (MQ=255)
                                                                                                                aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTAAGCCGGGCTGCAAGTGGCAGACCGTTAAAATAAACGGTTgggg                            >  3:209445/1‑149 (MQ=255)
                                                                                                                                          agagGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGGTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTc  >  5:38920/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
CGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC  >  NC_000913/379867‑380153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑