mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | EV-04-1-0_S14_L001_R1_001.good.fq | 2,008,999 | 500,885,438 | 100.0% | 249.3 bases | 282 bases | 98.9% |
| errors | EV-04-1-0_S14_L001_R2_001.good.fq | 2,008,999 | 505,513,373 | 100.0% | 251.6 bases | 282 bases | 87.0% |
| total | 4,017,998 | 1,006,398,811 | 100.0% | 250.5 bases | 282 bases | 92.9% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 203.6 | 9.4 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 6315 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 455 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.056 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.78334 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 12:32:09 26 Feb 2020 | 12:33:10 26 Feb 2020 | 1 minute 1 second |
| Read alignment to reference genome | 12:33:10 26 Feb 2020 | 12:53:25 26 Feb 2020 | 20 minutes 15 seconds |
| Preprocessing alignments for candidate junction identification | 12:53:25 26 Feb 2020 | 12:54:32 26 Feb 2020 | 1 minute 7 seconds |
| Preliminary analysis of coverage distribution | 12:54:32 26 Feb 2020 | 12:58:04 26 Feb 2020 | 3 minutes 32 seconds |
| Identifying junction candidates | 12:58:04 26 Feb 2020 | 12:58:12 26 Feb 2020 | 8 seconds |
| Re-alignment to junction candidates | 12:58:12 26 Feb 2020 | 13:03:22 26 Feb 2020 | 5 minutes 10 seconds |
| Resolving best read alignments | 13:03:22 26 Feb 2020 | 13:05:13 26 Feb 2020 | 1 minute 51 seconds |
| Creating BAM files | 13:05:13 26 Feb 2020 | 13:08:15 26 Feb 2020 | 3 minutes 2 seconds |
| Tabulating error counts | 13:08:15 26 Feb 2020 | 13:09:45 26 Feb 2020 | 1 minute 30 seconds |
| Re-calibrating base error rates | 13:09:45 26 Feb 2020 | 13:09:46 26 Feb 2020 | 1 second |
| Examining read alignment evidence | 13:09:46 26 Feb 2020 | 15:15:18 26 Feb 2020 | 2 hours 5 minutes 32 seconds |
| Polymorphism statistics | 15:15:18 26 Feb 2020 | 15:15:18 26 Feb 2020 | 0 seconds |
| Output | 15:15:18 26 Feb 2020 | 15:15:42 26 Feb 2020 | 24 seconds |
| Total | 2 hours 43 minutes 33 seconds | ||
