Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,511,665 T→C 7.5% I348V (ATT→GTT)  mntH ← manganese/divalent cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,511,6650TC 7.5% 167.5 / 3.8 58I348V (ATT→GTT) mntHmanganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base T (21/32);  new base C (3/2);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.44e-01

CTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTATGACCGGAAAAGTGGAACGCCGCCGC  >  NC_000913/2511384‑2511935
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                              
ctgcagaCCGGCGCGTTAACGCGTGGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCCGCCCCACCAACAACCGGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAAt                                                                                                                                                                                                                                                                                <  2:712113/276‑1 (MQ=255)
       ccGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTc                                                                                                                                                                                                                                                                         <  2:711619/282‑1 (MQ=255)
           ctctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGCGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGa                                                                                                                                                                                                                                                                      >  1:969458/1‑281 (MQ=255)
           ctctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGa                                                                                                                                                                                                                                                                      >  1:1289055/1‑281 (MQ=255)
           ctctTTAACGCGTAGGGCGGATAAGGAGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCAAGACCAGCACCACAATCACCCAGCCTGTCTGTTTCACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCCCTGGTGCAACTCAGCAGTGGAACAAGCGCCAGAGCGATACCAAAACTTACAAGTACCTGACTCAAAACCAGAATCCGGGTGGGa                                                                                                                                                                                                                                                                      >  2:276138/1‑281 (MQ=255)
            tctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAACCCGTGTc                                                                                                                                                                                                                                                                         >  1:713350/1‑277 (MQ=255)
            tctTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAACCCGTGTc                                                                                                                                                                                                                                                                         <  2:713350/277‑1 (MQ=255)
                     cgTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCgtg                                                                                                                                                                                                                                                                           <  2:833563/266‑1 (MQ=255)
                     cgTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCgtg                                                                                                                                                                                                                                                                           >  1:833563/1‑266 (MQ=255)
                        aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAg                                                                                                                                                                                                                                                        >  1:541443/1‑282 (MQ=255)
                           gcgagtAAGGCATTTACTCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAAt                                                                                                                                                                                                                                                     <  1:376819/277‑1 (MQ=255)
                           gCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAAt                                                                                                                                                                                                                                                     <  2:541443/282‑1 (MQ=255)
                                    gCGTTTACGCCGCATCCGCCAATCGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCGCCACCAACAACCAGATATCCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGAGTTTGCTGTTCACCAGATCGCCCATCAACTGGCTGTCAATGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGACACCAAACCTTAACAGTACCTGACCCATAACCAGGACCCGTGTCGGGCACACTCCCAACAGAAACACAATaaa                                                                                                                                                                                                                                            >  2:122348/1‑282 (MQ=255)
                                       tttACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGa                                                                                                                                                                                                                                         <  1:1118287/282‑1 (MQ=255)
                                       tttACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGa                                                                                                                                                                                                                                         <  1:667094/282‑1 (MQ=255)
                                         tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAAt                                                                                                                                                                                                                                                                <  1:963666/257‑1 (MQ=255)
                                         tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAAt                                                                                                                                                                                                                                                                >  2:963666/1‑257 (MQ=255)
                                            gccgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGc                                                                                                                                                                                                                                     <  1:459419/281‑1 (MQ=255)
                                            ccgccATCCGCCAAACATGCCAGATGCGACGCTCAGTCACCTACCATCCCGGCGCCGTTCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGc                                                                                                                                                                                                                                     <  2:156588/277‑1 (MQ=255)
                                                cATCCGCCAATAGTTCCCGATTCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCGGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACAt                                                                                                                                                                                                                                <  1:1233474/282‑1 (MQ=255)
                                                   ctcccAATAGAGCCCAACGGGACTCTCCTTTCACCACGACCCCAGCGCCGTCCCCCCCAACCACCAGATATTCAGCGCCTCGACCATCACCCCAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAAACAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGt                                                                                                                                                                                                                             <  2:379956/279‑1 (MQ=255)
                                                   ccGCCAATAGGGCCGAAGGCGACGCTCTTTCAACTTCAATCCCAGCGCCGTCCCCACCACCAACCGGATATTCAGGGCCACGACCATCACCAAAATGCCCCGACCTGTCTGTTTTACGCGTTTGCTGTTCACCAAATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGTAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCACCAGAATGACAATAAATGACGGCAACATGGt                                                                                                                                                                                                                             <  2:969458/282‑1 (MQ=255)
                                                   ccGCCAATAGGGCCAGATGCGACGCTCATGCAACTACAAGCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGt                                                                                                                                                                                                                             <  1:276138/282‑1 (MQ=255)
                                                     gCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGa                                                                                                                                                                                                                           <  1:314221/282‑1 (MQ=255)
                                                               ccAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCgtgt                                                                                                                                                                                                                                                                          <  1:1296951/225‑1 (MQ=255)
                                                               ccAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCgtgt                                                                                                                                                                                                                                                                          >  2:1296951/1‑225 (MQ=255)
                                                               ccAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacgacg                                                                                                                                                                                                                 >  1:1042965/1‑282 (MQ=255)
                                                               ccACATGGGACGGTCGTTGAACTACAAGCCCAGGGCCGGCCCCACCAACAACCAGAGTGTCCGCGACACGACCAGCACCACAACCACCCAGCCTGTCTGGGTGACGCGGTTGCTGTTCACCAGATCGCACATCAACTTGCTGTCACTGGTGAAAAGCAGCAGTGGAACCAGGGCGAGAGCTATACCATGACTTAACAGTACCTGACTCATAACCAGAATACGTGTCGGATCTAAGTCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacgacg                                                                                                                                                                                                                 <  2:1112381/282‑1 (MQ=255)
                                                                cAGATGCGACGCTCATTCAACTCCAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGc                                                                                                                                                                                                                <  1:1117807/282‑1 (MQ=255)
                                                                           cTCATTCAACTACACTCCCAGCGCCGTCCCCCCCCACAACCAGATATTCCGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTTTTTTACGCGTTTGCTGTTCACCCGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAg                                                                                                                                                                                                        <  2:854503/279‑1 (MQ=255)
                                                                           cTCATTCAACTACACGCCCCGCGCCGTCCCCACCAACAACCACGTATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAg                                                                                                                                                                                                        <  2:871349/279‑1 (MQ=255)
                                                                           cTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAg                                                                                                                                                                                                        <  1:177072/279‑1 (MQ=255)
                                                                           cTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCg                                                                                                                                                                                                      <  1:667237/281‑1 (MQ=255)
                                                                                 caactccctacccAGCGCCTGCCCCACCAACAACCCGAGAGTCAGCGCCACGACCATAACCACAAGCGCCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAAGCAGCAGGAGAACCAGCGCCAGAGCGATAACAAAACTTAACAGTACCTGAGTCATAACCAGGATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGatat                                                                                                                                                                                                <  2:28086/271‑1 (MQ=255)
                                                                                              atcgcCGTCCCCGCCAACATCCAGGCATTCAGGGCCACGACCGTAACCCCAATCACCCATCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCAATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGCGCGATACCAAAACTTCACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAg                                                                                                                                                                                  <  2:1289055/280‑1 (MQ=255)
                                                                                               gcgcCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAACCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAg                                                                                                                                                                                  >  1:631730/1‑281 (MQ=255)
                                                                                                    gTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTg                                                                                                                                                                             >  1:302047/1‑281 (MQ=255)
                                                                                                           accacaacCAGATGTGCAGAGCCACGACCAGAACCACAAGCACCCAGCCTGTCTGTTTTACGCGTTTGCAGTTCACCAGATCGCCCATCAACGTGCTGGCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTGAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATcacca                                                                                                                                                                     <  2:454490/279‑1 (MQ=255)
                                                                                                                aacCAGTAATTCCGCGCCCGAACCAGAACCCCAATCACCCAGCCTGTCTTTTTTAAGCGGTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAACCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGc                                                                                                                                                                <  2:631730/282‑1 (MQ=255)
                                                                                                                  ccAGATGTTCATCGCCACGGCCAGCACCACAATCACGCAGCCTGTCTGTTTTACCCGTTTGCTGTTAACCAGGGCGCCCATCAACTTTCTGTCACTGGTGAAAATCAGCAGTGGAACCAGGGCCAGAGCGATACCAAACCTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGccc                                                                                                                                                              <  1:24513/282‑1 (MQ=255)
                                                                                                                  ccAGATATTCAGCGCCACGACCAGCACCACAATCCCCCAGCCTGTCTGTTTTACGCGGTTGCTGTTCACCAGATCGCCCATCAACTTGCGGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTAACTCATAACCAGAATCCGTGTCGGATCTAATCCAATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGccc                                                                                                                                                              <  1:122348/282‑1 (MQ=255)
                                                                                                                    agttagtCAGCGACACGACTAGCACCACAATGCCCCGGCCTGTCGTTTTTACGCGTTTTCTGTTCCCCAAATCGCCCATCAACTTGTTGCCACTGGGGAAAAGCAGCAGTGGAACCAGCGCCAGGGCGATACCAAACCTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAATTGACGGCAACATGTTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGccc                                                                                                                                                              <  2:1428372/274‑1 (MQ=255)
                                                                                                                            aGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACCGCGGGATATGAAAGCGAATGAAGccc                                                                                                                                                                               >  1:379828/1‑255 (MQ=255)
                                                                                                                             gcgcCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTACACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACACCCTGCCCCGc                                                                                                                                                           >  1:1428372/1‑274 (MQ=255)
                                                                                                                                ccACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTc                                                                                                                                                    >  1:934769/1‑278 (MQ=255)
                                                                                                                                                                   tttACGCGTTTGCTGTTCACCAGATCCCCCATCAACTGTCTGGAACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCTTGTCGGATCTAATCCCATCAGAAGGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCtt                                                                                                             <  2:1042965/282‑1 (MQ=255)
                                                                                                                                                                                            cGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCca                                                                                                                                                                                                           >  2:281277/1‑163 (MQ=255)
                                                                                                                                                                                            cGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCca                                                                                                                                                                                                           <  1:281277/163‑1 (MQ=255)
                                                                                                                                                                                            cGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGc                                                                                                                                                                                          <  1:1110356/180‑1 (MQ=255)
                                                                                                                                                                                            cGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGc                                                                                                                                                                                          >  2:1110356/1‑180 (MQ=255)
                                                                                                                                                                                            cGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCaccacc                                                                                                                                                                   >  1:563151/1‑203 (MQ=255)
                                                                                                                                                                                            cGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCaccacc                                                                                                                                                                   <  2:563151/203‑1 (MQ=255)
                                                                                                                                                                                            cGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCgc                                                                                                                          >  2:562005/1‑244 (MQ=255)
                                                                                                                                                                                            cGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCgc                                                                                                                          <  1:562005/244‑1 (MQ=255)
                                                                                                                                                                                                                              cagTGGAAACAGCGCCAGAGCGATACCAAAACTTAATAGTACCTGACTCATAACCAGAATCCGTGTCGGAACTAACCTCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGTCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTCAACAGCGGATGAAACTGCAGATACGctt                                                   >  1:1188932/1‑279 (MQ=255)
                                                                                                                                                                                                                                        aGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTCACAGCGGTTGCAGCGTCAGATAAGCCCAATCAAGAtt                                         >  1:1147697/1‑280 (MQ=255)
                                                                                                                                                                                                                                                agCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATACGCCTCATCAAGATCGGCAacac                                 >  1:82805/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                               aTAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTATGACCGGAAAAGTGGAAc          >  1:110418/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                               aTAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAACGACGGCAACATGTTGACTGTACGACGCACCCACAGCGGCATCTGCAAGCGAATGAAGCTCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGACGACAGCCCCGCAGCAACCAGGCTTAATCCAACGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCACATAACCCTCATCCAGATCGCCAACACCAGTGTGCCCGCAAACGTGGAAccccgccgc  >  2:342169/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                              
CTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTATGACCGGAAAAGTGGAACGCCGCCGC  >  NC_000913/2511384‑2511935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑