| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,951,536 | Δ1 bp | pseudogene (977/984 nt) | ilvG → | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,951,534 | 0 | A | . | 97.6% | 177.9 / ‑1.6 | 43 | pseudogene (975/984 nt) | ilvG | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (17/24); minor base G (0/1); total (18/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTG > NC_000913/3951393‑3951663 | cGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACGAGCTGCGTCCGGCACATGTGGCAGTAGAAGGTGATTTAAACGCCCTGTTACCAGCATTCAAGCAGCCGTCaa > 2:146048/1‑143 (MQ=255) ggCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTTGTTTAGGTGCTCTGTCACCAGCGTCACAGCAGCCGTTaat > 1:281322/1‑142 (MQ=255) gCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaatc > 1:548131/1‑141 (MQ=255) cTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑CATCAATGa < 1:599195/143‑1 (MQ=255) cTGAACACCTTCGCGACACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGa < 1:500603/143‑1 (MQ=255) cacCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGgc < 1:569735/143‑1 (MQ=255) acgtgcgcgCCAGACGCCAGTGTTCTCCAGATGGAGATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGgca < 2:727383/138‑1 (MQ=255) ccTTCGCGCCACACGCCAGTATTATCCATATGGTTATCGACTCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑GATCAATAACTGgcgg > 1:598576/1‑141 (MQ=255) cTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGgcagc > 1:209941/1‑143 (MQ=255) ccgcgCCACCCGCCAGTGTGATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCaa < 1:644498/142‑1 (MQ=255) cacaCGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCAGCGTCAGGCACATGTGGCATCACAAGGGGATTTAAATGCTCTGTTACCAGCATTACACCAGCCGTT‑AAGCAATGACTGGCAGCAACACGgc > 2:516106/1‑143 (MQ=255) acGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTgcgcg > 1:687279/1‑143 (MQ=255) tgtTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGc < 1:659247/143‑1 (MQ=255) tgtTATCACTGTGGATATCGAGCCGGCAGAAATGAACAAGCGGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGc < 2:195244/143‑1 (MQ=255) tgtTAGCCATATGGAGATCGACCTGGCACAAATGAACAAGCGCCGTCAGGCACATGTGGCATTACAAGATGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGc < 2:611318/143‑1 (MQ=255) aTCCATAGGTATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGtga < 1:469344/143‑1 (MQ=255) atatGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACCGCACCCGCT‑ACTCAAGGACTGGCAGCACCACTGCCCGCAGCTGCGTGATGaa > 2:576412/1‑143 (MQ=255) atatGGATATCGACCCGGCAGAAACGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGaa < 1:663571/143‑1 (MQ=255) atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACa < 2:687279/143‑1 (MQ=255) gATAGCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTCGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTc < 2:209344/143‑1 (MQ=255) cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACCAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTAc < 1:537823/143‑1 (MQ=255) gaggcaaTGAACAAGCTGCGTCAGGCACATGTGGCATTACCAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGa < 2:238978/138‑1 (MQ=255) aGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCACCACTGCGCGCAGCTGCATGATGAACATTCCTGGCGTTACGAcc > 2:143319/1‑143 (MQ=255) gagcAAGCTGCGTCAGGTACATGTGGCATTAGAAGGTGATTTAAATGCTCTGGTACCAACATGATAGC‑GTTGGGCAATCAATGACTGCCACCAACACTGCGCGCAGCTGCGTCACTAACCTCGGCAGCGTAACGACCATCCCg > 2:191213/4‑143 (MQ=255) gAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCg > 1:741952/1‑143 (MQ=255) aaCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCgg < 1:464761/143‑1 (MQ=255) aaGCTGCGGCAGGGTCCTGTGCGATCACGAGGTGATTTAAATGCTCTGGTACCAGCAGTACAGCAGCCG‑TGAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGa < 2:519294/143‑1 (MQ=255) gTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACCGCGCGCAGCGGCGTGATGACCATTCCTGGCGTTACGACCATCCCGGTGACGCTATc > 2:159428/1‑143 (MQ=255) aTTAAAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgttgt < 2:31449/143‑1 (MQ=255) ttACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt < 2:431244/140‑1 (MQ=255) ttACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt > 1:431244/1‑140 (MQ=255) agacggTGATTTAAATGCTCTGTTACCAGCATGACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa < 1:575672/139‑1 (MQ=255) cAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaa > 1:70105/1‑143 (MQ=255) ggTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAgaca > 1:333684/1‑143 (MQ=255) tGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaacaac > 2:59303/1‑143 (MQ=255) tttAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGAGGCTATCTACGCGCCGTTGTTGTTAAAACAACTGt < 1:562402/143‑1 (MQ=255) tgtAAATGCTCTGTGACCAGCACTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGCTGAACATTCCTCGCGTTACGAACATCCCGGTGCCGCTATCTACGCGCCGTTGTTGTTAAaacaaccaa < 1:686113/141‑4 (MQ=255) ttAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGGTAAAACAACTGTc > 1:148318/1‑143 (MQ=255) ttAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGAAGCTGCGTGATGAACATTCCTGGCGGTACGACTATCCCGGTGACGCTAGCTACGCGCCGTTGTTGGTAAAACAACTGTc > 1:147587/1‑143 (MQ=255) aTGCTCTGTTACCAGCATCACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCCGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGAt < 1:670637/143‑1 (MQ=255) gCTCTGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCg > 1:495558/1‑143 (MQ=255) tctGTTACCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTATTCTTTTGATAACGCTCGGATCGtg > 2:566430/1‑142 (MQ=255) ggTTACCAGCATTACAGCAGCCGTT‑AATCAATGCCTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTaaa < 1:435558/142‑1 (MQ=255) gTTACCAGCATTAAAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCCGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAc < 1:431821/143‑1 (MQ=255) aCCAGCATTACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACGGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAGACCTg < 2:12302/143‑1 (MQ=255) ttACAGCAGCCGTT‑AATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGGTGTTGACACCACTCTCGGCTCGTAACCATGCggctca > 2:144466/1‑139 (MQ=255) | CGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTG > NC_000913/3951393‑3951663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |