New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 334914534 (0.820)4 (0.100) 4/276 5.7 11.2% coding (665/729 nt) mtgA biosynthetic peptidoglycan transglycosylase
?NC_000913 = 3349179 30 (0.740)coding (631/729 nt) mtgA biosynthetic peptidoglycan transglycosylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAG  >  NC_000913/3349014‑3349283
                                                                                                                                   |                                                                                                                                          
cTATTCCCCTGCAGAAAGATCTGCACCCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACgg                                                                                                                                 <  1:508430/143‑1 (MQ=255)
 tATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGGTTAATCCAGCGGGTGTTGTTGCATAAACGGCGCATCGCCTAACTGATACATCTACCGTAAAATCCACGCCTGACggg                                                                                                                                >  1:163577/1‑142 (MQ=255)
              aaaGATCTGCAACCTCGTGAGAGCAAAACGCGGGCGCGCACTGCGCGCGAAGCGTAGTCCAGCTGGTGTTGTTGCATAAACGGCCCACCGCCTAACTGATACAGCGGCCGGAAACTCCACGCCGGAGGGCCACGCACGTAGcc                                                                                                                   >  2:622366/1‑143 (MQ=255)
                  aTCTGCAACCGCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGTGCGTAGccgtca                                                                                                               <  2:509511/143‑5 (MQ=255)
                  aTCGGCGACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGAt                                                                                                               <  2:45208/143‑1 (MQ=255)
                   tctcCAACGTCGTCGGATCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGGTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATg                                                                                                              <  2:453600/143‑1 (MQ=255)
                       cAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCga                                                                                                          <  1:608120/143‑1 (MQ=255)
                        aaCTTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCgag                                                                                                         <  1:268467/143‑1 (MQ=255)
                        aaCCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCgag                                                                                                         <  1:648363/143‑1 (MQ=255)
                               ggagaGCAAAACGCTGGCCCTCGCTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTGGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACtt                                                                                                  <  2:410520/142‑1 (MQ=255)
                                gagaGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACttt                                                                                                 >  1:419634/1‑143 (MQ=255)
                                        aaCGCTGGCCCTCGCTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGaa                                                                                         <  1:418011/143‑1 (MQ=255)
                                        aaCGCTGGCCCTCACTTCGCGCGCAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGCCGGCTACACACGTACCGCGATGACGAGGAGACGTTCAAACGat                                                                                         >  2:79276/1‑142 (MQ=255)
                                                  cTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATACACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTCGCCCGATGGCGAGGCGACGTTGAAACGAAGAGGACTAgc                                                                               >  2:75638/1‑142 (MQ=255)
                                                       ttCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGCGACTTTGAAACGAAGTGGATTAGGTAATa                                                                          >  1:125792/1‑143 (MQ=255)
                                                                  cTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGCCTTTGAAACGACGTGGATTAGGGAATAGAGCTGCCAGt                                                               >  2:426972/1‑143 (MQ=255)
                                                                  cTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGt                                                               >  1:271429/1‑143 (MQ=255)
                                                                  cTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGCGGATTAGGTAATACAGCTGCCAGt                                                               >  1:96225/1‑143 (MQ=255)
                                                                      gtCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATg                                                           <  1:723584/142‑1 (MQ=255)
                                                                          aGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGCTGCTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAgg                                                       >  1:45606/1‑142 (MQ=255)
                                                                                          tAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTCGCCCGATGGCGACGCGACTTTGAAACGAAGTGGATTAGGTAATACATCTGCCAGTAATGCAGCTTCCGACCGGGaaaat                                       >  1:529917/1‑141 (MQ=255)
                                                                                          tAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAGTACAGCTGCCAGTAATGCAGCTTCCGCCCGGGTAAGt                                       >  1:600919/1‑143 (MQ=255)
                                                                                           aaaCGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGtt                                      >  1:741933/1‑143 (MQ=255)
                                                                                               ggatCACCGCCTAACTCATACATCTGCCGTAAATTCCCCGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCt                                  <  2:33767/140‑1 (MQ=255)
                                                                                                    aCCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGCCTTTGAAACGAAGCGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCCcgcgc                             >  2:133756/1‑142 (MQ=255)
                                                                                                    aCCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCgg                             >  1:230421/1‑143 (MQ=255)
                                                                                                    aCCGCCTAACTGATACAGCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCgg                             <  2:709888/143‑1 (MQ=255)
                                                                                                            aCTGATACATCTGCCGTAAAATACACGCATGACGGGTACGCACGTAGCACGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCGAGTAATGCAGCTTAGGAGCGGGTAAGTTTGCTAGCGGGAtggtgg                     >  1:257511/1‑143 (MQ=255)
                                                                                                                    aTCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCGGTAATGCAGCTACCGACAGGGGAAGTTAGCTCGCGGGGTTGTGGAaacaaca             >  2:514860/1‑142 (MQ=255)
                                                                                                                         ccGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTtgtg        <  2:52328/143‑1 (MQ=255)
                                                                                                                            tAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCATTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCg     >  2:373970/1‑143 (MQ=255)
                                                                                                                               aacccAGGCGTGGCGGGTACGCAGGTAGGCCGAGGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAg  <  1:305414/140‑1 (MQ=255)
                                                                                                                               aaTCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTTTGGAACTACCGTTGTGCCGCAg  >  2:494191/1‑143 (MQ=255)
                                                                                                                               aaTCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAg  >  1:252977/1‑143 (MQ=255)
                                                                                                                                   |                                                                                                                                          
CTATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAG  >  NC_000913/3349014‑3349283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.