Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	51 (1.230)	37/282	0.1	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	63 (1.520)	43/282	0.0	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCAT  >  NC_000913/2289974‑2290248                                                                                                                                            |                                                                                                                                        gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATa                                                                                                                                      >  1:552737/1‑143 (MQ=32)  aaaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGCTCGCACCTTCCATag                                                                                                                                     >  2:607439/1‑142 (MQ=14)   aaaTCTCCTTTCTGGGCTGACGGCGCTTACTGCTGAATTTACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGACGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAAc                                                                                                                                    <  2:726528/143‑1 (MQ=255)    aaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACg                                                                                                                                   <  2:453861/143‑1 (MQ=34)      tctcTTTTCTGGTCTGACGGCGCTCACTGCTGAATTCACTGGCGGCGAAGGTATGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGGTCGCATATCAGGGACTTATTCGGACCTTCCATagggag                                                                                                                                 >  1:612793/1‑138 (MQ=11)         ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTACTGCCGTTACCAGTGCTAAAGAAGGCTATTTGATGCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                              <  1:759944/143‑1 (MQ=9)         ctTTTCTGGTCTGACGGCGCTTACTGCTGAACCCGCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                              <  1:315527/143‑1 (MQ=255)              cttgTCTGACGGCTCTTACTGCTGAATTCACCGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                         <  1:496429/140‑1 (MQ=255)                ggTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTCGccaccc                                                                                                                       >  1:574966/1‑142 (MQ=255)                    tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAAc                                                                                                                   >  1:676507/1‑143 (MQ=255)                           gcTTACTGCTGACCTCACTCTCGGCGAAGGTAAGTTGATGGCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATAtt                                                                                                            <  1:255841/143‑1 (MQ=255)                                 tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGATCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCGCCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGaa                                                                                                      >  2:586556/1‑143 (MQ=255)                                 tgctgAATTAACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACATTCCATAACGCTGTAGCCACCGAGACAGATAATGCGGaa                                                                                                      >  2:168347/1‑143 (MQ=17)                                        tgcACTGTCCACGAAGGCAACTTGAAGACTCACGATGAACCCGCTTCTATGGCTCCAGATGCCAAACATGAGCTCATAGCAGGGACTGGTTCGCACCTTCCATATCGCTGTAGCTACCATAACAGATATTGCGGAACGACaaa                                                                                               <  1:258663/141‑1 (MQ=255)                                          gaCTGTCGGCGAAGGTAAGCGGATGACGCATGATGAACCCTGTGCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGGGCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAaga                                                                                             <  2:345345/142‑1 (MQ=18)                                             tGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaa                                                                                          >  1:367102/1‑143 (MQ=255)                                                      aGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGAAGTGGCTATACGCTTTGAGGTGGCGGAACGACAAAGAGAAACAGAACCa                                                                                 <  1:725174/143‑1 (MQ=14)                                                          aaGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGAtt                                                                             <  1:644866/143‑1 (MQ=255)                                                          aaGTTGATGACGCATGATGAACCCTGTTCGATGTCTCCAGATGACAAACATGGTCTCAGATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGAtt                                                                             <  2:476774/143‑1 (MQ=25)                                                             ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCGTATCAGGGACTTGTTCGCACCTGCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGAt                                                                          <  2:688093/143‑1 (MQ=255)                                                                       atgatgAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCAACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCt                                                                >  2:475604/1‑143 (MQ=255)                                                                         gacgaACCCTGTTCTATGGCCCCAGATGACAAACATGATCGCATATCAGGGACTTGTTCGCACCGTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCttt                                                              <  1:651950/140‑1 (MQ=255)                                                                            gaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACAATGTAGCCACCAGAACAGCTATTGCGGAACCACAAAGAGAAACAAAACCAGAGACATGCACTAAGCTGTCAt                                                           >  2:270445/1‑143 (MQ=21)                                                                             aaCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATc                                                          <  2:676507/143‑1 (MQ=255)                                                                                 cTGTTCTATTGGTCCAGATGACAAACCTGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGGCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTAt                                                      <  2:653933/143‑1 (MQ=255)                                                                                 cTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTAt                                                      >  1:178631/1‑143 (MQ=255)                                                                                     tCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGaaa                                                  <  1:482124/143‑1 (MQ=255)                                                                                      cTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaat                                                 >  1:320865/1‑143 (MQ=255)                                                                                           gCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaatt                                            >  1:123302/1‑143 (MQ=255)                                                                                               cAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTg                                        <  1:37408/143‑1 (MQ=255)                                                                                                aGATGACAAACATGATCTCATATCAGGAACTTGTTATGATCTTTCATAACGCTGTAGCCACCAGAACAGATATTGCAGAACGTCAAGGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCGATGAAATTAATAGCTGt                                       >  2:306495/1‑143 (MQ=255)                                                                                                 gATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGtt                                      >  1:463047/1‑143 (MQ=255)                                                                                                       aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTACATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACAAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaacg                                >  2:751580/1‑141 (MQ=255)                                                                                                            tGATCTCATATCAGGGACATGTAAGCACGTTCCATAACGCTGTCGCCACCAGAACAGATATTGCGGAACGACAAATAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTgg                           <  1:103608/143‑1 (MQ=255)                                                                                                                 tcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGACCGACAAAGAGAAACAGAACCAGATTGATGCATCGAGCTTTCATCCTATGAAATTAATTGCTCTTCAACGCATTGCGcaca                      >  2:329561/1‑143 (MQ=255)                                                                                                                  cATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAg                     >  2:529183/1‑143 (MQ=255)                                                                                                                        aGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATa               >  1:102769/1‑143 (MQ=255)                                                                                                                            aCTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCa           <  1:459385/143‑1 (MQ=255)                                                                                                                                  tCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTc     <  1:529183/143‑1 (MQ=255)                                                                                                                                   cGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTcc    <  2:474262/143‑1 (MQ=255)                                                                                                                                   cGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACAAGATTCCTGCAGTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTcc    >  1:248451/1‑143 (MQ=255)                                                                                                                                     cACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACTCATAGCTCCAt  >  1:483764/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                        GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCAT  >  NC_000913/2289974‑2290248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.