Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	66 (1.640)	38/282	0.1	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	49 (1.220)	32/282	0.3	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

ACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAG  >  NC_000913/2101617‑2101878                                                                                                                                     |                                                                                                                                  aCATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGa                                                                                                                         <  2:184070/143‑1 (MQ=255)   aTAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAAt                                                                                                                       <  1:677916/143‑1 (MQ=255)   aTAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCAGTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAAt                                                                                                                       >  2:170979/1‑143 (MQ=255)     aaaGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATCTTTGTTTTATTAAGCAACGTGCGAACaa                                                                                                                     >  2:322132/1‑143 (MQ=255)     aaaGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTGAGTAATTTTTGTTTTATTGAGGTAGTTTCGACTaa                                                                                                                     >  2:174879/1‑143 (MQ=255)         tAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgg                                                                                                                 <  1:237515/143‑1 (MQ=255)         tAACCGTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgg                                                                                                                 <  1:135325/143‑1 (MQ=255)            ccTTGATCAAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGa                                                                                                              <  2:417505/143‑1 (MQ=255)              ttGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGaaa                                                                                                            <  2:301379/143‑1 (MQ=255)               tGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAt                                                                                                           >  1:683074/1‑143 (MQ=255)                 aTCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAttc                                                                                                         <  2:81792/143‑1 (MQ=255)                    gAAGCCATTAACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTGTATTAAGGAAGGTGCGAATAAGCGGGGAAAttcttc                                                                                                      <  2:522399/143‑1 (MQ=255)                          aTTTACACGCACACACTCTGAAAAACGTACCAACATAAATGATCCTGCGCACCAATCAACATACGCTTCAGAAGTGATACTTTCTTTAGGAATTTTTGTCTTATTAACGACGGTGCGAATAAGCGGGGAAATTCTTCTCGGCt                                                                                                <  2:396587/143‑1 (MQ=255)                          aTTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAACTTCTTCTCGGCt                                                                                                >  2:422459/1‑143 (MQ=255)                                acGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                          >  1:329967/1‑143 (MQ=255)                                            tGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTcatttcattt                                                                              <  1:194317/143‑1 (MQ=255)                                               aaaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTCTGTTTGATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                           <  1:273444/143‑1 (MQ=255)                                                aaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATGGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttcttc                                                                          <  2:712562/143‑1 (MQ=255)                                                  acatACCCGCATGAATGCTCCTGCGAGCCAGTCAACAACCGTATCACAATAGACACTTTCTTTAGTAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGGCATTTCATTTCTTCAt                                                                        <  2:682192/140‑1 (MQ=21)                                                     tACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGtt                                                                     <  2:368010/143‑1 (MQ=255)                                                        cAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGCATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                  <  2:561050/143‑1 (MQ=255)                                                         aGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTGATGTTTGAg                                                                 >  1:3519/1‑143 (MQ=255)                                                         aGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAg                                                                 >  1:633378/1‑143 (MQ=255)                                                          gtttacaTGATCCTGCGCACCAATCAACAACCGTAGCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGc                                                                <  1:547887/137‑1 (MQ=255)                                                           cATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGCGCAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGcc                                                               >  1:664450/1‑143 (MQ=255)                                                               aaTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGATTTATTAAGGAAGGGGCGAATAAGCGGGGAAATTCTTCTCGGCTGAATCAGTCATTGCAATTCTTCATGTTTGAGCCGAtc                                                           >  2:583646/1‑142 (MQ=25)                                                                aTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGTAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttt                                                          <  1:74532/143‑1 (MQ=255)                                                                 tGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttt                                                         >  1:687195/1‑143 (MQ=255)                                                                      cTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                    <  1:527696/143‑1 (MQ=255)                                                                             ccAATGGACAACCGTATCAGAATAGATGCGTTCTGTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTGTGAGCCGATTTTTTCTCCCGTaaa                                             <  2:625365/143‑1 (MQ=18)                                                                               aaTCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATg                                           >  1:371257/1‑143 (MQ=255)                                                                                aTCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAATGTGCGAATAAGCGGGGAAATTCTTCTCTGCTGACTTAGTCATTTCATTTCTTCCTTTTTGAGCCGCTTTTTTCTCCCGTAAATGc                                          >  2:434351/1‑143 (MQ=21)                                                                                    acaacCGTATCAGAATAGATACTTTCTTTAGGACTTTTTGTTTTATTAAGGAAGGAGCGAATAAGCGGGGCAATTCTTCTCGGCTGACTCACTCATTTCATTTCTTCATGTTTGAGCCGATCTTTTCTCCCGTAAATGCCttc                                      >  1:598134/1‑142 (MQ=18)                                                                                     caacCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGa                                     <  1:61623/143‑1 (MQ=255)                                                                                      aacCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCCGTCACGTCAGGGCGTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTgca                                    >  1:694771/1‑141 (MQ=21)                                                                                           taccAGAATAGATACTTTCTTTACGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTCACCCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                               <  1:51116/140‑1 (MQ=255)                                                                                           tATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                               <  2:487078/143‑1 (MQ=255)                                                                                                gAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAGATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTTCTCCCGTAAATGCCTTGCATCAGCCTAtt                          >  2:246585/1‑143 (MQ=255)                                                                                                 aaTAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAttt                         <  2:683074/143‑1 (MQ=255)                                                                                                      aTACTTTCTGGAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                    <  2:462868/143‑1 (MQ=255)                                                                                                             ctttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTCTAGACCGTttctt             >  1:235005/1‑143 (MQ=255)                                                                                                                 aGGAATTGTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGcc         <  1:117576/143‑1 (MQ=255)                                                                                                                        tttGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAg  >  1:72454/1‑143 (MQ=38)                                                                                                                                     |                                                                                                                                  ACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAG  >  NC_000913/2101617‑2101878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.