| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 22.9% | 88.9 / 25.5 | 61 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (20/27); new base T (9/5); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTA > NC_000913/4295918‑4296200 | tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAAc < 2:493819/143‑1 (MQ=32) gCGTTTATGCCGCGTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCt < 1:148442/143‑1 (MQ=17) gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCTTCCGTCGTCACTGCCTGa > 1:292551/1‑143 (MQ=11) tGCCGCCCCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCACCCGACATCAACGCCTGATGCGa < 1:597363/143‑1 (MQ=12) gccgccCCCCACCTCACCGCCTGAAGAGTCGCTTCACGCTTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGAc < 1:23569/143‑1 (MQ=11) gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCACGCCTACGCCAGACAG‑CGCAATAGCCCGATTTCGCCTGATCTTCCAAGTCGCCTCAAGCGT‑TTACGCCGTATCAGACCTCAACGCCTGaatcacc > 2:409617/1‑137 (MQ=11) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACTCGAGACAT‑CGCAATAGCCTGATTTCGCGTGATTTTGTAGGAAGGATAAGGCGA‑TTACGCCGCATCGGACATCAACGACAGATgcggcg > 2:94974/1‑143 (MQ=11) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGAt < 1:164322/137‑1 (MQ=18) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGAt > 2:164322/1‑137 (MQ=18) cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGACGcgccgc > 2:370089/1‑143 (MQ=12) ctCCGGCATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATGGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTg < 2:90957/142‑1 (MQ=255) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc < 1:56892/143‑1 (MQ=34) tgtCATCGCCGGAGGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTGGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt < 2:407711/141‑1 (MQ=14) cgtCAACGCCTGATGCGACGCTTACCGCGTCGTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt < 2:99070/141‑1 (MQ=255) cATCAACGCTTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt < 1:192727/143‑1 (MQ=38) accacggCCTGCTCCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAAGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc < 1:310919/137‑1 (MQ=11) aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc > 1:86051/1‑143 (MQ=25) aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTTCCGCGTCTTa > 2:612826/1‑143 (MQ=25) aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa > 1:514600/1‑143 (MQ=25) aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt > 2:266423/1‑143 (MQ=25) ccTGATGCGACCCTTACCGCGTCTTATCAGGGCTACGCCAGACCG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCAACATCAACGCCTGATGCGCCGCTTGCCGTGTCTTATCAg < 2:54394/143‑1 (MQ=11) cGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc > 1:272214/1‑141 (MQ=17) cGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCCg > 1:25460/1‑143 (MQ=37) gtcGCTTAACTCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc < 1:292040/141‑1 (MQ=37) gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAccc > 2:242205/1‑141 (MQ=37) cgccgtaCGCGTCTTATCAGGCTTACGCCAGGCAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑CTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa < 2:510171/137‑1 (MQ=14) cgattACCGCGTCTTATCAGGCGTACGCGAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa < 2:510412/140‑1 (MQ=255) cTTAACGCGTCTTATCAGGCCTACGCCAG‑CAGTCCCGGCAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa < 1:132843/143‑1 (MQ=14) ttAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACCCCTGATGCGACGCCTGACGCGGCCTATCACGCCCACTCCCGAc > 2:694601/1‑143 (MQ=11) tgaCGCGCCTTATCAGGCCTACGCCAGGCAG‑CGCAATAGCCTGATTTAGCGGGATTTCGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa < 2:732516/141‑1 (MQ=255) gcgTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc < 1:111829/143‑1 (MQ=38) cgTCTTAGCAGGGCTACGTCAGGGGG‑CGCAATGGCGTGACTTAGCGTGATTTTGTGGGGCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCa < 2:107475/143‑1 (MQ=11) gTCTTATCAGCTCTACGCCAGAGAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa < 2:308134/143‑1 (MQ=255) tCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 1:26429/124‑1 (MQ=25) tCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc > 2:26429/1‑124 (MQ=40) atgaGGCCTACGCCAGACGG‑CGATATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAGGGCGT‑TTCCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc < 2:647344/140‑1 (MQ=14) gCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCGTGGTTTCGCGt > 2:47917/1‑143 (MQ=255) ccAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTg > 1:562733/1‑143 (MQ=39) cgACTG‑CGCAATGGCCTGATTTAGCGTGATTATGTAGGTGGGGTGCGGCGT‑AGCCGCCCAATCCGCTATGATCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAt < 1:136196/142‑1 (MQ=11) gACAG‑CGCAATAGTCTGGTTTCGCGTGATTTAGT‑GGGGGCAGAACCATTCCTACGCCGCATCCGAGATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtt < 1:332986/143‑1 (MQ=9) aCAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGATGCGACGc < 1:537625/85‑1 (MQ=18) aG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt < 2:538601/92‑1 (MQ=255) aG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt > 1:538601/1‑92 (MQ=255) g‑cgcAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGACAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt < 1:375093/143‑1 (MQ=255) g‑cgcAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGACAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt < 1:660575/143‑1 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa < 1:363027/143‑1 (MQ=39) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGCCAGCGCAAAAGCCTGCTTTAGCGGGATTTCGTAGGGCgggt > 2:408774/1‑141 (MQ=11) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGTGTTTAACGACACCTACCCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGAt > 1:728803/1‑143 (MQ=14) tGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa < 1:486169/143‑1 (MQ=255) cGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa > 1:357727/1‑143 (MQ=38) ttttGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg > 1:366400/1‑143 (MQ=39) tttGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGACTCTGTAGGTCGGATAAGGCGTTTATGCCGc > 2:167208/1‑143 (MQ=255) tAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATcc > 1:253292/1‑143 (MQ=38) cGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAAACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACAt < 2:366400/143‑1 (MQ=255) aTAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCac > 1:196959/1‑142 (MQ=38) tAAGTCGT‑TTACGCCGCATCCGAAATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc < 1:94974/143‑1 (MQ=255) tAAGGCGT‑TTCCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc < 1:272292/143‑1 (MQ=38) cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTCTGTAGGTCGGATACGGCGTTTATGCCGCATCCGACATCCACGCCTGATGCGACGc > 2:567052/1‑143 (MQ=255) cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCCCATCCGACATCAACGCCTgacgcgacgcgt > 1:30472/1‑141 (MQ=255) cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGTCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCttcccg > 2:605148/1‑139 (MQ=255) gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGCTTATGCCGCATCCGGCATCAACGCCTGATGCGACGCTTGAcgc > 1:585039/1‑143 (MQ=255) aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTGATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTa > 1:335046/1‑143 (MQ=12) | TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAG‑CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGT‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTA > NC_000913/4295918‑4296200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |