Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,978,503 Δ776 bp insB1insA insB1, insA

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978503–1979270 1979278 9–776 68 [0] [0] 68 insB1–insA insB1,insA

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 19785020 (0.000)63 (1.200) 49/264 0.1 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein

TTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913/1978366‑1978644
                                                                                                                                        |                                                                                                                                              
tttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataag                                                                                                                                          >  1:866021‑M1/1‑137 (MQ=255)
tttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATAACCAGGAGGGGCTCAGCGGTTTTTACGGTATTTAAATGCAGATCACACAAAACACTCAATTACTTAACATAAATgtataag                                                                                                                                          >  2:214178/1‑137 (MQ=255)
  tACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtc                                                                                                                                        <  2:352271‑M1/143‑9 (MQ=255)
     acTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACGCGCACCTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcata                                                                                                                                     >  1:939771‑M1/1‑132 (MQ=255)
        tATCTAAGATTGTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGCGGCTGCGACTTTCAATAATGCGTGATGCAGATCACACACATTCGTCAATTGGTTAACATAAATGtataagtcatactt                                                                                                                                  <  2:456325‑M1/143‑15 (MQ=255)
            tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatgcttttgt                                                                                                                              >  1:406951‑M1/1‑125 (MQ=255)
            tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgt                                                                                                                              >  1:772492‑M1/1‑125 (MQ=255)
            tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgt                                                                                                                              >  1:478846‑M1/1‑125 (MQ=255)
            tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGCGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAATAGtaaaagtcatacttttgt                                                                                                                              >  2:62483‑M1/1‑125 (MQ=255)
            tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACCCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTCATTAACATAAATGtataagccatacttttct                                                                                                                              >  2:892308‑M1/1‑125 (MQ=255)
                     tCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTATCGTGCTTGtataagtcatacttttgctttgggtgt                                                                                                                     >  1:954044‑M1/1‑116 (MQ=255)
                       cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtat                                                                                                                   >  1:186928‑M1/1‑114 (MQ=255)
                         aaaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatat                                                                                                                 >  2:557355‑M1/1‑112 (MQ=255)
                           aTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACCTAAATGtataagtcatactattgttttgggtgtatttca                                                                                                               >  2:572057‑M1/1‑110 (MQ=255)
                            tCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtaatacttttgttttgggtgtatttaaa                                                                                                              >  1:975979‑M1/1‑109 (MQ=255)
                              gACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttatgggtgtatttcaatc                                                                                                            >  2:221435‑M1/1‑107 (MQ=255)
                                 gCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGCTTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                                                                         >  1:968025‑M1/1‑104 (MQ=255)
                                        tACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtctaagtcatacttttgttttgggtgtatttcaatctgttaaaaag                                                                                                  >  1:871156‑M1/1‑97 (MQ=255)
                                           tCGTCACTACACGCAGATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttt                                                                                               >  2:854909‑M1/1‑94 (MQ=255)
                                           tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttt                                                                                               <  1:924846‑M1/143‑50 (MQ=255)
                                           tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttt                                                                                               >  2:286791‑M1/1‑94 (MQ=255)
                                             gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttccatctgtaaaaaagatttt                                                                                             >  1:480635‑M1/1‑92 (MQ=255)
                                              tCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                                                            <  1:191851‑M1/143‑53 (MQ=255)
                                               cACTACACGCACATGCACGGGAGGGAGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATGACTTAACATAAATGtataagtcatacttttgttgtgggtgtatttcaatctgttaaaaagtttttcg                                                                                           <  2:954829‑M1/143‑54 (MQ=255)
                                                     acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgc                                                                                     <  1:405407‑M1/143‑60 (MQ=255)
                                                     acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgc                                                                                     >  1:952738‑M1/1‑84 (MQ=255)
                                                       gCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                                                                   >  1:836405‑M1/1‑82 (MQ=255)
                                                         acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagc                                                                                 <  1:237590‑M1/143‑64 (MQ=255)
                                                         acaTACAACGGAGGGAGCCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagc                                                                                 <  1:268681‑M1/143‑64 (MQ=255)
                                                            tACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaag                                                                              >  2:252518‑M1/1‑77 (MQ=255)
                                                               aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaa                                                                           >  2:328286‑M1/1‑74 (MQ=255)
                                                                       ggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtattttaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                   <  2:772492‑M1/143‑78 (MQ=255)
                                                                       ggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgcgtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                   <  2:478846‑M1/143‑78 (MQ=255)
                                                                        gggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaac                                                                  >  2:520620‑M1/1‑65 (MQ=255)
                                                                        gggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaac                                                                  >  1:20409‑M1/1‑65 (MQ=255)
                                                                            tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAGATAAACGgacaagtcatacttttgttgtcggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagga                                                              >  2:987335‑M1/1‑61 (MQ=255)
                                                                            tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtgttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagga                                                              >  1:171900‑M1/1‑61 (MQ=255)
                                                                              cGATTTTCAATAATGCGTGATGCAGATCACACAACCCTCTTCATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcagtctgataaaaagtttttcgctacgctagcaagcaaaaatgaaacacgaat                                                            >  2:990284‑M1/1‑59 (MQ=255)
                                                                                 ttttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataat                                                         <  1:46662‑M1/143‑88 (MQ=255)
                                                                                   tctaataatgcgTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttctctaccctagcaatcaaaaatgaaacaggaataatcg                                                       <  2:173178‑M1/140‑90 (MQ=255)
                                                                                    tCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaacgtttttcgctacgctagcaagcaaaaatgacacaggactaatcga                                                      >  1:129884‑M1/1‑53 (MQ=255)
                                                                                        taatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatg                                                  >  1:870523‑M1/1‑49 (MQ=255)
                                                                                          atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggg                                                <  2:177722‑M1/143‑97 (MQ=255)
                                                                                          atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggg                                                >  1:731540‑M1/1‑47 (MQ=255)
                                                                                            gCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaatcaggaataatcgaaatgggat                                              <  1:286791‑M1/143‑99 (MQ=255)
                                                                                            gCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat                                              <  1:904243‑M1/143‑99 (MQ=255)
                                                                                                  tGCAGATCACACAAAACACTCAATTACTTTACATAACTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcg                                        <  2:681189‑M1/143‑105 (MQ=255)
                                                                                                        tCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaccgt                                  >  1:20348‑M1/1‑33 (MQ=255)
                                                                                                            acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggaatgttgcacacagtcaaa                              >  2:244682‑M1/1‑29 (MQ=255)
                                                                                                            acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                                                                        <  1:760245‑M1/101‑73 (MQ=255)
                                                                                                            acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                                                                        >  2:760245‑M1/1‑29 (MQ=255)
                                                                                                            acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtattgggtgtatttcaatctgttaaaaagtttttagctacgctagcaagcaaaaatgaaacaggaataatcgaactgggatgtggcgcacaggcaaa                              >  2:140709‑M1/1‑29 (MQ=255)
                                                                                                             caAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgatgcgcacagtcaaaa                             <  2:855113‑M1/143‑116 (MQ=255)
                                                                                                                agcgttcaagtACTTAACCTAAATGtataggtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                          <  2:98916‑M1/138‑119 (MQ=255)
                                                                                                                aaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaagtgggatggtgcgcacagtcaaaataa                          >  2:864547‑M1/1‑25 (MQ=255)
                                                                                                                       aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccg                   <  1:996704‑M1/143‑126 (MQ=255)
                                                                                                                       aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccg                   >  1:136112‑M1/1‑18 (MQ=255)
                                                                                                                          tACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatagaaatgggatgttgcgcacagtcaaaataactcaccgtaa                <  1:520620‑M1/143‑129 (MQ=255)
                                                                                                                           aCTTAACATAAATGtataagtcattcttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaa               <  1:420116‑M1/143‑130 (MQ=255)
                                                                                                                            cTTAACATAAATGtataagtcatacttttgatttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaaccggcagaagcgaaatgggagggagagctgtgtcaaaagaactcaccgtaaag              >  1:632152‑M1/1‑13 (MQ=255)
                                                                                                                             ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaacta             >  2:591038‑M1/1‑12 (MQ=255)
                                                                                                                             ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaata             >  2:248645‑M1/1‑12 (MQ=255)
                                                                                                                             ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaata             >  2:248574‑M1/1‑12 (MQ=255)
                                                                                                                                aCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatc          <  1:363118‑M1/143‑135 (MQ=255)
                                                                                                                                  aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataattat        >  1:412183‑M1/1‑7 (MQ=255)
                                                                                                                                     aaTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaaaaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctg     <  2:968025‑M1/143‑140 (MQ=255)
                                                                                                                                       tGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttttgctacgctagccagacccctccaaacaggaataatcgaaatgggctgttgcgcccagtcaaaataactcaccgtcaataatcatctgct   >  1:743322‑M1/1‑2 (MQ=255)
                                                                                                                                        gtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta  <  1:854909‑M1/143‑143 (MQ=255)
                                                                                                                                        |                                                                                                                                              
TTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913/1978366‑1978644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: