Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA98.2% 151.7 / NA 54P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (24/29);  minor base G (1/0);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC  >  NC_000913/4184404‑4184679
                                                                                                                                           |                                                                                                                                        
cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATATCTAAGACCAACCCGATCAAACCTATGCCTAACCAGCACAACCATACGCCGGTAGACACACCACTGAACCAGcg                                                                                                                                       >  2:493428/1‑142 (MQ=255)
  ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                     <  1:593861/143‑1 (MQ=255)
    cGGTTAGACGCCGTATCCAGCCTGGGGACAAGATGGCAGGTCGGCAGGGTAACAAGGGTGTAATTTCTAAGATCAACACGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                   <  1:433118/143‑1 (MQ=255)
    cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCATTACAATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                   <  1:43284/143‑1 (MQ=255)
     ggTTAAACGCCGTATCCAGCCAGGAAACAAGATGGCAGGTCGTCACGGTAACAAGGGAGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAACGTACTGAACCAGCTGGCCg                                                                                                                                  >  2:526800/1‑143 (MQ=255)
      gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGAACAACCCGATAGAAGATATGCCTTGCGATGAAAACGGTAGGCCGGTAGACATGGGACTGAAGCAGCTGGGCGt                                                                                                                                 >  1:825985/1‑143 (MQ=255)
             gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACACAGCTGCGCGTACCGtct                                                                                                                          >  2:958132/1‑143 (MQ=255)
                 tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGGGTAATTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                      >  2:237261/1‑143 (MQ=255)
                   tCCAGCCTGGTGACAATATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                    <  1:980047/143‑1 (MQ=255)
                     cAGCCTGGTGCCAAGACGGCAGGCCGTCACGTTAACCAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                  <  1:924786/143‑1 (MQ=255)
                     cAGCCTGGTGACGAGATGGCAGGTGGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGAGGAAAACGGTACGCCGGTAGACACCGTACTTAACTAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                  <  1:696882/143‑1 (MQ=255)
                          ttgTGACAAGAGGGTAGGTCGTCCCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                             <  2:310272/141‑1 (MQ=255)
                                cAAGATGGCAGGCCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGa                                                                                                       <  1:318450/143‑1 (MQ=255)
                                 aaGATGCCAGGTCGTCACGGTAACAGGGGTGTAATGTCTAAGATCAACCCGCTCGAAGATATGCATTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCAGGTCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                      <  2:305703/143‑1 (MQ=255)
                                     tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGCATGAACATCGGTCCGATCCTc                                                                                                  >  2:231566/1‑143 (MQ=255)
                                          ggTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAc                                                                                             <  1:858279/143‑1 (MQ=255)
                                           gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                               >  2:886609/1‑108 (MQ=255)
                                           gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                               <  1:886609/108‑1 (MQ=255)
                                              gtgaCGGTAACCAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                         <  2:707767/140‑1 (MQ=255)
                                                cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                       <  1:674159/143‑1 (MQ=255)
                                                    gTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGt                                                                                   <  1:571094/143‑1 (MQ=255)
                                                         aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAACCGGTACGCCGGTAGACATCGTCCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCCGATCCTCGACAGCCACCCGGGTATGGc                                                                              >  1:795189/1‑143 (MQ=255)
                                                         aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                              >  1:496134/1‑143 (MQ=255)
                                                           gggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCGGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTg                                                                            >  1:513604/1‑143 (MQ=255)
                                                            ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGCAGACATCGTACTGAACCAGCTGGGCATACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGCATGGCTGc                                                                           >  2:676790/1‑143 (MQ=255)
                                                              tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                         >  1:858540/1‑143 (MQ=255)
                                                                tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATGGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTGTGGCTGCGgaa                                                                       >  1:618536/1‑143 (MQ=255)
                                                                tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATACACGAAACCCACCCGGGTATGGCTGCGaaa                                                                       >  2:917602/1‑143 (MQ=255)
                                                                tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGCTCCTCCAAACCCACCTGGCTACGGCTGCGaaa                                                                       >  2:805190/1‑143 (MQ=255)
                                                                 aaTTTCTAAGACCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGCCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                      <  1:70410/143‑1 (MQ=255)
                                                                       tAAGATCAACCCGATCGAGGATATGCCTTACGATGAAAACGGTACGCCTGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                <  1:958132/143‑1 (MQ=255)
                                                                              cccccGATCGAAGATATGCCTTACGAGGAAAACGGGAAGCCGGTAGCCAGCGGACTGAACCAGCGGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                         <  1:549312/141‑1 (MQ=255)
                                                                               aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                        <  1:407232/143‑1 (MQ=255)
                                                                                  cGAGCGAAGATATGCCCTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                     <  1:712349/143‑1 (MQ=255)
                                                                                   gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGAGAAAGGTATCGGCGACAAGAtaa                                                    >  1:350443/1‑141 (MQ=255)
                                                                                        aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCCACGcc                                               >  2:53909/1‑143 (MQ=255)
                                                                                               gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTACCGGCGACACGATCAACGCCATGCCGa                                        >  2:279782/1‑143 (MQ=255)
                                                                                                ccTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaa                                       >  1:562313/1‑143 (MQ=255)
                                                                                                 cTTACGATGGAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                      <  2:561466/143‑1 (MQ=255)
                                                                                                    accaTGACAAAGGAAAGCCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                   <  1:371581/140‑1 (MQ=255)
                                                                                                    aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                   <  1:60889/143‑1 (MQ=255)
                                                                                                      gATGAAAACGGTACGCCGGTAGACCTCGGCAACAACGAGATGGACGTACCGTCTCGTATGAACATCGGTCGGAACCCCTAATCCCACCTGGGGATGCCTTCGAAGGGTATCGGCTACGGGAACAACGCCATGCTGAAAcagca                                 >  2:19117/1‑143 (MQ=255)
                                                                                                       aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                >  2:642073/1‑143 (MQ=255)
                                                                                                           aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCAGAAACAGCAGCaag                            >  2:901275/1‑143 (MQ=255)
                                                                                                              ctgTACGCCGGTAGACCTCGTACTGAACCAGCTGGGCGCACCGTCCCGTATGAACATCGGTCAGATCCTCGAAACCCTCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                         <  2:951953/141‑1 (MQ=255)
                                                                                                              cgttACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                         <  1:299647/140‑1 (MQ=255)
                                                                                                                gTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                       <  1:1012241/143‑1 (MQ=255)
                                                                                                                 tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAGACCCACCTGGGTATGGCTGCGGAAGGTATCGGCGACAAGATCAATGCCATGCTGAAACAGCGGCCAGAAGTcc                      >  1:469669/1‑142 (MQ=255)
                                                                                                                         tAGACATCGTACTGAACCAGCGGGGAGTCGCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCGGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTg              <  2:273231/143‑1 (MQ=255)
                                                                                                                            aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCCCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc           >  2:119446/1‑143 (MQ=255)
                                                                                                                                cgaaCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCGGATCCTCGAAACCCACCTGGGGATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt       <  2:212818/140‑1 (MQ=255)
                                                                                                                                   aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                           >  2:884266/1‑120 (MQ=255)
                                                                                                                                   aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCGGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                           <  1:884266/120‑1 (MQ=255)
                                                                                                                                     tGAACCAGCTGGGCGGACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc  <  1:465084/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC  >  NC_000913/4184404‑4184679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑