| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,164,899 | G→A | R359C (CGC→TGC) | parC ← | DNA topoisomerase IV, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,164,899 | 0 | G | A | 95.5% | 53.7 / NA | 22 | R359C (CGC→TGC) | parC | DNA topoisomerase IV, subunit A |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (6/15); minor base T (0/1); total (6/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CGACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCGCACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTC > NC_000913/3164759‑3165038 | cgACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGCGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGACGGcaca > 2:695608/1‑143 (MQ=255) gcgcCGGTTTCGGTTCATCCTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATcg < 1:837136/143‑1 (MQ=255) ttCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGGGGATATGCAGGCGCTTGAGGGCTTTCTCCAGCCGATAGTTCAGTCGCGGACACTCGGTGTCGCGGCGGACCACCCGCCAtt > 2:759735/1‑143 (MQ=255) ttCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGCCCTTCGCCAAACGATAGTTCAGTCGGCGGCACACGGTCTCGCGGCGGAGCCCCAGCCAtg > 2:116398/1‑142 (MQ=255) aaTCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCa < 1:397894/119‑1 (MQ=255) aaTCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCa > 2:397894/1‑119 (MQ=255) tCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAgg < 1:565937/143‑1 (MQ=255) agaAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCAGAGGAACACCAGCCAATCCGAGAGGGTTTACAGGAGGATGTTCACCGCCGgaca > 1:727036/1‑142 (MQ=255) tcaGCAAACCTTCGTGGCTTTGCTGGCGCTTGTGGATTTTCTCTACTCTATATTTCAGTCGGCGTCACATGTTATCGCTGCTGAACACCAGCCATTCGGAGAGGATTTCCAGTAGGTTTTTCACCGCCGGACGACCATCCAGa < 2:563038/142‑1 (MQ=255) aaaCCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGAt < 1:330825/143‑1 (MQ=255) tggATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAg < 2:325471/142‑1 (MQ=255) cgtatatGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGTCTCACGTTATCGCAGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAg < 2:818812/140‑1 (MQ=255) aGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAg < 1:880909/143‑1 (MQ=255) ggATATGCAGGCGCTTGAGGAGTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAgg < 1:264669/143‑1 (MQ=255) gcgcTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGAt < 1:889793/143‑1 (MQ=255) gAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGGTTCTCACCGCCGGACGACGGTCCGGAGCGACGAGACAAAGGCTAATGCGCTAGctcg > 2:442999/1‑142 (MQ=255) ggACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCttt < 1:116398/143‑1 (MQ=255) gTTCAGTCGGCGGCACACGGTAGCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGa < 1:458544/143‑1 (MQ=255) ttCCGTCGGCGTCACACGGTATCGCGTCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAATGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa < 2:1011610/143‑1 (MQ=255) tccatccggcggCAGACGTTACCGCGGGGGACCAACAGCCATTCGCAGAGGATTTCCAGCAGGTGTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa < 2:642610/137‑1 (MQ=255) gTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgg < 1:917116/143‑1 (MQ=255) tCGGCGGCATACGGGATCGCGGCGGAGCACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggt < 2:742547/143‑1 (MQ=255) tCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATGCGATGGCTCTTTGCCAGATCGGTGGCGGCGCAGCggt > 2:360772/1‑143 (MQ=255) gcggcaCACGGGATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggtggt < 1:314918/143‑1 (MQ=255) ggcaCACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGGTTCTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtc < 1:846838/143‑1 (MQ=255) | CGACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCGCACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTC > NC_000913/3164759‑3165038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |