Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258675 258683 9–776 32 [0] [0] 31 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)28 (0.760) 22/460 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

CATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAA  >  NC_000913/258424‑258948
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                        
acgcttaatcacctcatcatctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGataacc                                                                                                                                                                                                                                                            >  2:178612‑M2/261‑273 (MQ=255)
    tttatctcctcatcaactgctgacggtaaattattagagatcccatttatcccggtgtatcggaattttgacaaaggtcttcatgaaatcaccgactggatcgaaaaacgcgtgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                                                                                                                                                                                         <  1:310617‑M2/18‑1 (MQ=255)
     taatcacctcatcatctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatgtggtaaaacagttgcatcacaacaggagatagcaatgacgttaacgagtggacacccgaagagcagattgagcaaaaaatttaccgcactaggcccgtatattggtgaaggtaAGTGCAAAGATAATCGa                                                                                                                                                                                                                                                          >  1:368384‑M2/256‑272 (MQ=255)
                 tactggctgacggtaaattattatagatcccaattaacccggtttagcggaagtttgacaaaggtcttcaggaaatcaccgactggttcgaaaaacgcttgggttaaaaatttgctaaattttaccaatttggtaaaacagttgcatcacaacaggagctagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactagacccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGTTTGt                                                                                                                                                                                                                                           <  2:368384‑M2/32‑1 (MQ=255)
                  atttgctgacggtaaattgttagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcatgaaatcaccgactggatagaaaaacgcttgtgttaaaaatttgctaaattttggcaatttggtaaaacagttgcatcacaacaggagatagcaatgactttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                                                                                                                                           <  1:249034‑M2/32‑1 (MQ=255)
                  atctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                                                                                                                                           <  1:4038‑M2/32‑1 (MQ=255)
                                aattattagagaacccagttaaccaggtgtatctgaattttgacaaaggtcttcaggaaatcaccgacctgatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacagtagatatcaaggacgttaccgagtggacccccgaagagcagattgatcaaaaaatttaccgcactaggcccgcatattcgtgaaggtaAGTTCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTc                                                                                                                                                                                                                            <  1:178612‑M2/47‑1 (MQ=255)
                                             cccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgccctaggcccgtatattagtgacggtaAGTGCAAAGATAATCGATTCTTTTTCGAGTGTCTGGATGTATGCGTCAACGTGAAACcgt                                                                                                                                                                                                               >  2:218682‑M2/216‑274 (MQ=255)
                                                          gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                                                                                                                                                     >  1:121361‑M2/203‑272 (MQ=255)
                                                          gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                                                                                                                                                     >  1:385965‑M2/203‑272 (MQ=255)
                                                           tgtatcggaatttttacaaacgtcttcagtaaatcaccgactggagcgaaaaacgcttgtgttaaaaatttgctacattttgccaatttgttaaaacagttgcatcacaacaggagatagcaatgacgttaccgggtggacacccgaagagcagattgatcaaaaaatttaccgcactagtcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGTCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                                                                                                                                                 <  1:218682‑M2/74‑1 (MQ=255)
                                                           tgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                                                                                                                                                 >  1:346128‑M2/202‑275 (MQ=255)
                                                                               ggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                                                                                                                                             <  2:121361‑M2/94‑1 (MQ=255)
                                                                                ggcttcaggaaataaccgactggatcgaaaaacgcttgttttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtgtacacccgaagagcagattgatcaaaaaatttaccgtactaggcccgtatattcgtgaatgtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGGCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                                                                                                                                             <  2:384945‑M2/94‑1 (MQ=255)
                                                                                                             aaccgcttgtgttaaaaagttgctaaattgtgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTGGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                                                                                                                                               <  1:362066‑M2/124‑1 (MQ=255)
                                                                                                                 gcttgttttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTa                                                                                                                                            <  1:267940‑M2/127‑1 (MQ=255)
                                                                                                                                                atttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAg                                                                                                            <  2:346128‑M2/159‑1 (MQ=255)
                                                                                                                                                  ttgggaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGc                                                                                                          <  2:385965‑M2/161‑1 (MQ=255)
                                                                                                                                                  gtggtaaaaaagtgtcagcacaacagtagatagcagtgacgttaccgagtggacacccagagagcagattgatcaaaaaatgtaccgcactaggcccgtatcttcgtgaaggtaAGTGCAAAGATAATCCATTCTTTTTCGATTGTTTGGCTGTATGCTTCAAAGTGAACCCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGGTTGGTCTGTTCGATAAATCAGGc                                                                                                          <  2:45455‑M2/161‑1 (MQ=255)
                                                                                                                                                               tgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAgtg                                                                                             >  2:125851‑M2/102‑275 (MQ=255)
                                                                                                                                                                                      tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACAGTGAAGTg                                                                      >  1:146192‑M2/79‑275 (MQ=255)
                                                                                                                                                                                          gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACt                                                                                                      >  2:311959‑M2/75‑239 (MQ=255)
                                                                                                                                                                                              ccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGaa                                                                                                  <  1:311959‑M2/169‑1 (MQ=255)
                                                                                                                                                                                                ggatggacacccgaaggtcagatggatcaaaaaagttaccgtgctaggcccggagattcgtgaaggtaAGGGCAAAGATAATCGATTCTTTTTTGATTGTCTGGCTGGATGCGTCAACGTGAAACCGGCACTGGATGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGGTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCTGTAAAAGACACTGAAGTGGTTGAACGAc                                                            <  2:214488‑M2/207‑1 (MQ=255)
                                                                                                                                                                                                     gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGc                                                                                                              >  2:37968‑M2/64‑220 (MQ=255)
                                                                                                                                                                                                         cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGc                                                                                                          <  1:37968‑M2/161‑1 (MQ=255)
                                                                                                                                                                                                                    gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTtaa                                                                                                                                     >  1:197317‑M2/49‑181 (MQ=255)
                                                                                                                                                                                                                        gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGt                                                                                                                                 <  2:197317‑M2/138‑1 (MQ=255)
                                                                                                                                                                                                                                                          cgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACATACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACGCTGCGTGAGTTTCACGAGAAGCTGGGTGAAGtggtgacgacggcgaa  >  1:145006‑M2/11‑270 (MQ=255)
                                                                                                                                                                                                                                                              aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGa                                                                                                                                                                                                                     >  1:150701‑M2/7‑60 (MQ=255)
                                                                                                                                                                                                                                                                  taAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                                                                                                                                                 <  2:150701‑M2/58‑1 (MQ=255)
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                        
CATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAA  >  NC_000913/258424‑258948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.