Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,244,371:1 +C 5.6% coding (705/1545 nt) malF ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,3711.C 5.6% 296.2 / 11.2 72coding (705/1545 nt)malFmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base . (35/33);  new base C (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

TGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTG  >  NC_000913/4244092‑4244653
                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           
tGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTgg                                                                                                                                                                                                                                                                                    <  1:429965/289‑1 (MQ=255)
     ccGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTAt                                                                                                                                                                                                                                                                                >  1:62958/1‑288 (MQ=255)
       gcgTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCg                                                                                                                                                                                                                                                                             <  2:61688/289‑1 (MQ=255)
        cgTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCgg                                                                                                                                                                                                                                                                            >  1:11228/1‑289 (MQ=255)
        cgTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCgg                                                                                                                                                                                                                                                                            >  2:10622/1‑289 (MQ=255)
         gTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGa                                                                                                                                                                                                                                                                           <  1:320429/289‑1 (MQ=255)
                  aaTCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACCGGTAAAAGCCAATTTGGTTCTTCGGACGAAAttt                                                                                                                                                                                                                                                                   >  2:299129/1‑288 (MQ=255)
                     cagcagGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCAcg                                                                                                                                                                                                                                                               <  2:87427/289‑1 (MQ=255)
                     cagcagGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCAcg                                                                                                                                                                                                                                                               <  2:11228/289‑1 (MQ=255)
                       gcagGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAACGCCAATTTGGTTATTCGGACGTTATTTCAcgcc                                                                                                                                                                                                                                                             >  1:263417/1‑289 (MQ=255)
                            aCGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCAGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGctct                                                                                                                                                                                                                                                        >  1:269749/1‑289 (MQ=255)
                             cgcgATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTg                                                                                                                                                                                                                                                       >  1:179042/1‑289 (MQ=255)
                                                       gCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGt                                                                                                                                                                                                                             <  2:381040/289‑1 (MQ=255)
                                                                            gCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGa                                                                                                                                                                                                                          <  1:169805/271‑1 (MQ=255)
                                                                            gCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGa                                                                                                                                                                                                                          >  2:169805/1‑271 (MQ=255)
                                                                                      tGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGaaa                                                                                                                                                                                               >  1:493533/1‑289 (MQ=255)
                                                                                      tGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGaaa                                                                                                                                                                                               >  1:421803/1‑289 (MQ=255)
                                                                                      tGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGc                                                                                                                                                                                                                                      >  2:426598/1‑249 (MQ=255)
                                                                                      tGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGc                                                                                                                                                                                                                                      <  1:426598/249‑1 (MQ=255)
                                                                                      tGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGaaa                                                                                                                                                                                               >  1:325519/1‑288 (MQ=255)
                                                                                           aCCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGCAAACTGgc                                                                                                                                                                                         >  1:478969/1‑289 (MQ=255)
                                                                                              gCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCa                                                                                                                                                                                      <  1:10622/289‑1 (MQ=255)
                                                                                               ccACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCa                                                                                                                                                                                      <  2:421803/289‑1 (MQ=255)
                                                                                               ccAACGTTAACAAGACAGTGATCAGCGAGAACACCACGGTCAAGACGAAAAGGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCa                                                                                                                                                                                      <  2:493533/289‑1 (MQ=255)
                                                                                                cACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAgg                                                                                                                                                                                    <  1:11229/289‑1 (MQ=255)
                                                                                                   cattAAAAAGACAGTGTACAGCGAGAACACCACGTTCCAGGCGAAAATGGCGGGGAACGGTTTCTGAATGCCGTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTACTCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAg                                                                                                                                                                                 <  2:322137/287‑1 (MQ=255)
                                                                                                   cGTTAAAAAGACGGTGATCAGCTGAAACACCACGGTCCAGACGAAAATGGCGAGAAACGGTGTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCTGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATAGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTTATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAg                                                                                                                                                                                 <  1:472544/289‑1 (MQ=255)
                                                                                                             aCAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCtcatcatc                                                                                                                                                                        >  2:132406/1‑288 (MQ=255)
                                                                                                                        cGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCCCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCCCCTAATCCCTTTGTTGCCa                                                                                                                                                            >  2:147863/1‑289 (MQ=255)
                                                                                                                           gaACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATc                                                                                                                                                          <  2:325519/288‑1 (MQ=255)
                                                                                                                             acacCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCg                                                                                                                                                        >  2:184426/1‑288 (MQ=255)
                                                                                                                             aaaccacGGTCCAGGCGAAAATGGCGAGGAACGGTTTCTGAATGCGTTGGGCGGTAAGGACGCGTGTAAAGTTGTGCCAGCGGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCAGCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCAGGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCgg                                                                                                                                                       <  2:154131/287‑1 (MQ=255)
                                                                                                                                        cAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCgg                                                                                                                                            <  2:179042/289‑1 (MQ=255)
                                                                                                                                         aGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCCGTTGCCGTCGGCGGTAATGGACTGGTCAACGCCAATTTGGTTATTCGGACGATCTTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGCGCGGCTGCGTGCCGGAAAACTGGCACAGGGAGCTCCTCATCTCTTTGTTGCCATCCGTCAGACTGGCTGt                                                                                                                                           >  2:450054/1‑289 (MQ=255)
                                                                                                                                                aaTGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGa                                                                                                                                                   >  2:361674/1‑274 (MQ=255)
                                                                                                                                                aaTGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGa                                                                                                                                                   <  1:361674/274‑1 (MQ=255)
                                                                                                                                                         gAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCaa                                                                                                                                                                                                                                                                                         <  2:298013/131‑1 (MQ=255)
                                                                                                                                                         gAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCaa                                                                                                                                                                                                                                                                                         >  1:298013/1‑131 (MQ=255)
                                                                                                                                                                 tCTGAATCCCGTCGGCGGTAAAGACGCGGGTAAAGTTATTCCAGCCGGTGGTCAGGGTGTAACCGGGGCTTAGCTTTTCATAACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGCTTATTCGTCAACGGGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGAtt                                                                                                                   <  2:263417/289‑1 (MQ=255)
                                                                                                                                                                  cTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTc                                                                                                                  <  1:184426/289‑1 (MQ=255)
                                                                                                                                                                         ccTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCAcg                                                                                                                                                                                                                                                               <  1:17088/141‑1 (MQ=255)
                                                                                                                                                                         ccTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCAcg                                                                                                                                                                                                                                                               >  2:17088/1‑141 (MQ=255)
                                                                                                                                                                                    aaaGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGa                                                                                               <  1:132406/289‑1 (MQ=255)
                                                                                                                                                                                                    gttttGCAGCCGGGGGTGAAGGTGTAACGGGGTGTGAGTATGTCATCACCCCAGTTGCCGTCGGCATTAATGGACTGGTAAAAGCCAATTTGATTGTTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACTGGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCt                                                                                <  2:478969/287‑1 (MQ=255)
                                                                                                                                                                                                             ccGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATCCGTCAACGTGCCGTCACCGCCGAGCGTGTAGAGCGGCTGCCTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACCCAGCGCCTGACGATTCTGGGTAATCCCGGCGCGACTCGCTCCTTCGCCCTCGGGCTggg                                                                      >  2:21246/1‑289 (MQ=255)
                                                                                                                                                                                                                          tgtAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATc                                                                                                         >  1:402341/1‑242 (MQ=255)
                                                                                                                                                                                                                          tgtAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATc                                                                                                         <  2:402341/242‑1 (MQ=255)
                                                                                                                                                                                                                                  gggCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCa                                                 >  2:115362/1‑289 (MQ=255)
                                                                                                                                                                                                                                  gggCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCa                                                 >  1:142827/1‑289 (MQ=255)
                                                                                                                                                                                                                                   ggCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAg                                                                                                                                                                                 >  2:71821/1‑161 (MQ=255)
                                                                                                                                                                                                                                   ggCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAg                                                                                                                                                                                 <  1:71821/161‑1 (MQ=255)
                                                                                                                                                                                                                                    gCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGt                                               >  1:477842/1‑289 (MQ=255)
                                                                                                                                                                                                                                    gCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACCCAGCGCCTGACGATTCCGGGTAATTACGCGCAGATTCGCCCGGTCGCCTTCGGGCTTGGCCGCCGTTTCCTTCAGCTGcccg                                               >  2:257157/1‑286 (MQ=255)
                                                                                                                                                                                                                                        aGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGttttt                                           >  2:390884/1‑289 (MQ=255)
                                                                                                                                                                                                                                               catcaCCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGa                                                                                                                        <  2:236741/206‑1 (MQ=255)
                                                                                                                                                                                                                                               catcaCCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGa                                                                                                                        >  1:236741/1‑206 (MQ=255)
                                                                                                                                                                                                                                                  caCCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCgccgcc                                 <  1:303716/289‑1 (MQ=255)
                                                                                                                                                                                                                                                  caCCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCAGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCgccgcc                                 <  1:299129/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    ccccAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATc                                                                                                                                                          <  2:294565/167‑1 (MQ=255)
                                                                                                                                                                                                                                                    ccccAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATc                                                                                                                                                          >  1:294565/1‑167 (MQ=255)
                                                                                                                                                                                                                                                    ccccAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGGCGTTTTTGCCCGCCGCCac                               >  2:228945/1‑288 (MQ=255)
                                                                                                                                                                                                                                                         gTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTa                          >  1:366043/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          tggCCGTCAGGGGTAAGGGACTGGTAAAAGCCAATTTGGTAATTGGGACGTTGTTTCACGCCGGTCTGATTATTCGTCAACGTGCCGTGACCGTCGAGTGTGTAGAGCGGCGGCGTGCCGGAAAACTGGCGAAGGGAGCTCATCATCACTTTGTGGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTGCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaa                         <  2:62958/287‑1 (MQ=255)
                                                                                                                                                                                                                                                               gTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATAtt                                                                                                                                                                                                                                                                    <  2:18014/50‑1 (MQ=255)
                                                                                                                                                                                                                                                               gTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATAtt                                                                                                                                                                                                                                                                    >  1:18014/1‑50 (MQ=255)
                                                                                                                                                                                                                                                                     ggTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGga               <  1:115362/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                     ggTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGga               <  1:228945/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCg                 <  1:218727/284‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 gTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTTATGTAACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGCCTGGGCGGTCGTTTCTTGCAGTTGCAGTTTTTGCTCGCCTCCAAATTTAAAAGCGCTGGAGAGGTAATTTTTg  >  2:383210/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                 gTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCCCGCGCAGATTCGCGCGTTCGCCTTCGGGCTTGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCCGCGCCCAGTTTAAAAGCGTCGGAGAGGTaaatctg   >  2:218727/1‑283 (MQ=255)
                                                                                                                                                                                                                                                                                 gTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTGCGGCTGGGCGGTCGTTTCTTTCAGTTGCAGCTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGACGGGTAAttttt   >  2:488867/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                 gTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAttttt   >  1:116674/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           
TGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTG  >  NC_000913/4244092‑4244653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: