Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,450,334	G→T	8.5%	P86Q (CCG→CAG)	yfcP ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,450,334	0	G	T	8.5%	162.6 / 6.0	47	P86Q (CCG→CAG)	yfcP	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

CGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGATTTATTCATTGATAATCCACCCGTAATGTTGCGTA  >  NC_000913/2450069‑2450616                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                           cGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTa                                                                                                                                                                                                                                                                     >  2:412817/1‑289 (MQ=255)   cAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTaaa                                                                                                                                                                                                                                                                   >  2:490457/1‑289 (MQ=255)   cAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTaaa                                                                                                                                                                                                                                                                   <  1:367347/289‑1 (MQ=255)                ggTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACt                                                                                                                                                                                                                                                               <  1:1749/280‑1 (MQ=255)                ggTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACt                                                                                                                                                                                                                                                               >  2:1749/1‑280 (MQ=255)                        cACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGa                                                                                                                                                                                                                                              <  1:318185/289‑1 (MQ=255)                         aCCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATTCCAATTCCCACTCCCTGAGCGGCTGATGACGTATCCAGTGCCACAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGCGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTCCAATCTTTCAGCTCAAAGACTACGGGTACACGAGCggt                                                                                                                                                                                                                                              >  2:120318/1‑287 (MQ=255)                                  cacaTCACGGCCGCTTTTCGCCGGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCa                                                                                                                                                                                                                                    <  1:39884/289‑1 (MQ=255)                                       cACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGATCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCTGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGt                                                                                                                                                                                                                                >  2:37028/1‑288 (MQ=255)                                          ggCCGCTTTTCGCCATGAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCaa                                                                                                                                                                                                                            <  1:120318/289‑1 (MQ=255)                                                                              tatCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGc                                                                                                                                                                                        <  1:412817/289‑1 (MQ=255)                                                                                 cGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCCTGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTCCAGCTTAAAGACTACGGGTACACGAGCGGACAGGCCTGCACCGTTCAAATCCTGCCTACTGACGCTGGGCAAGCGCACCTCTCCCAg                                                                                                                                                                                     >  2:299124/1‑289 (MQ=255)                                                                                       tGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCttt                                                                                                                                                                                                                                                                            <  1:335045/196‑1 (MQ=255)                                                                                       tGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCttt                                                                                                                                                                                                                                                                            >  2:335045/1‑196 (MQ=255)                                                                                          cATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             <  1:490457/288‑1 (MQ=255)                                                                                                  aGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATccacc                                                                                                                                                                     >  1:487935/1‑288 (MQ=255)                                                                                                         aGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCTGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCa                                                                                                                                                                           <  2:433889/275‑1 (MQ=255)                                                                                                         aGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCTGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCa                                                                                                                                                                           >  1:433889/1‑275 (MQ=255)                                                                                                                          ttgttgATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             >  1:202341/1‑256 (MQ=255)                                                                                                                          ttgttgATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             <  2:202341/256‑1 (MQ=255)                                                                                                                                  ggCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCg                                                                                                                                    >  2:224702/1‑289 (MQ=255)                                                                                                                                       ccTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCg                                                                                                                                    >  1:312372/1‑284 (MQ=255)                                                                                                                                       ccTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCg                                                                                                                                    <  2:312372/284‑1 (MQ=255)                                                                                                                                              tCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGa                                                                                                                        <  2:487935/289‑1 (MQ=255)                                                                                                                                                  tCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCg                                                                                                                                    >  2:274159/1‑273 (MQ=255)                                                                                                                                                  tCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCg                                                                                                                                    <  1:274159/273‑1 (MQ=255)                                                                                                                                                                                tGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             <  1:262683/202‑1 (MQ=255)                                                                                                                                                                                tGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             >  2:262683/1‑202 (MQ=255)                                                                                                                                                                                tGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCAtt                                                                                       >  1:35245/1‑288 (MQ=255)                                                                                                                                                                                        tgatgaCGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATc                                                                              >  2:28747/1‑289 (MQ=255)                                                                                                                                                                                           tgaCGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGc                                                                           <  1:224702/289‑1 (MQ=255)                                                                                                                                                                                                 aTCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             <  1:343271/185‑1 (MQ=255)                                                                                                                                                                                                 aTCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             >  2:343271/1‑185 (MQ=255)                                                                                                                                                                                                    cAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCTGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAAc                                                                  <  1:37028/289‑1 (MQ=255)                                                                                                                                                                                                       tGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAgcgc                                                                                                                                                             >  2:225465/1‑195 (MQ=255)                                                                                                                                                                                                       tGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAgcgc                                                                                                                                                             <  1:225465/195‑1 (MQ=255)                                                                                                                                                                                                             aaaaCCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACg                                                            >  2:325175/1‑286 (MQ=255)                                                                                                                                                                                                              aaaCCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGt                                                           <  1:325175/286‑1 (MQ=255)                                                                                                                                                                                                                           cGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAAt                                           <  2:35245/289‑1 (MQ=255)                                                                                                                                                                                                                              tGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCAt                                        >  1:54407/1‑289 (MQ=255)                                                                                                                                                                                                                               gTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGCCTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCCCTGCCAGATATGGGCCATCCACCACCAGCGCCCAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCCAATAACGTGCTCCTCATTGCCAATACCAGCCCCGCTAAAAGGCTGTAACCACGCGACTGCTTCAtt                                       >  2:290665/1‑289 (MQ=255)                                                                                                                                                                                                                                  ttctACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGAt                                    <  1:28747/289‑1 (MQ=255)                                                                                                                                                                                                                                     tACCCCCTGGGGCGTAACTTTGACGTTGTATCCCGCCGGCCCTTTGCAATCGTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGATTTa                                 <  1:290665/289‑1 (MQ=255)                                                                                                                                                                                                                                                               tGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATccaccacca                                                                                                                                                                 <  1:448084/135‑1 (MQ=255)                                                                                                                                                                                                                                                               tGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATccaccacca                                                                                                                                                                 >  2:448084/1‑135 (MQ=255)                                                                                                                                                                                                                                                                gTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGATTTATTCATTGATAATCCACCCGTAATGtt       >  1:345263/1‑288 (MQ=255)                                                                                                                                                                                                                                                                    cccGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGATTTCTTCATTGATAATCAACCCGTAATGTTGCGtc  >  2:469266/1‑288 (MQ=255)                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                           CGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGATTTATTCATTGATAATCCACCCGTAATGTTGCGTA  >  NC_000913/2450069‑2450616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑