Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA96.0% 221.8 / NA 76P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (49/23);  total (51/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAT  >  NC_000913/4184407‑4184678
                                                                                                                                         |                                                                                                                                       
gCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTggg                                                                                                                                   >  1:112367/1‑143 (MQ=255)
   gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGCTCGCCAGTTCGAGGTACACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGt                                                                                                                                >  1:1081572/1‑143 (MQ=255)
    ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACACACTACTGAACCAGCAGGGCgga                                                                                                                               >  2:1073354/1‑141 (MQ=255)
    ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGGAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                               >  2:962352/1‑143 (MQ=255)
       aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATAGGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACAGAACCAGCTAGGCGTACCg                                                                                                                            >  2:560941/1‑143 (MQ=255)
        aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                           >  1:121762/1‑143 (MQ=255)
        aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCAAAGATCAACGCGATCGAAGATATGGATTAAGATGAAGATGGTACTCCGCTAGCGATGGTACTGAACCAGCTGGGAGTAGCGt                                                                                                                           >  2:70741/1‑143 (MQ=255)
        aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGATGTAATTTCTAAGATCAACACGATCTAAGATATGCCTTACGATAAAAAAAGTACGACGGTAAACATCGTACTGAAACAACTGAGCGAACCGt                                                                                                                           >  2:529513/1‑143 (MQ=255)
              tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGGACGCCGGTAGACATCGTACGGCACCAGCTGGGCGTACAGTCTCGTa                                                                                                                     >  2:97031/1‑143 (MQ=255)
               aTCCAGCGTGGTGACACGATGGCAGGTCGTCCCGGTAACAAGCGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAGAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                    <  2:661502/143‑1 (MQ=255)
               aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGCGCGTCCCGTCGCGTAt                                                                                                                    >  2:776356/1‑143 (MQ=255)
                 ccAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACCATGAAAACGGTACGCCGCTAGACCTCGTACCGACCCCGCTGGGCGTACCCTCTCGtctca                                                                                                                  >  1:1015229/1‑139 (MQ=255)
                  cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTTCCGTCTCGTATGaa                                                                                                                 >  1:734428/1‑143 (MQ=255)
                         gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACTGTCTAGTATGAAAATCGGt                                                                                                          >  1:279262/1‑143 (MQ=255)
                         gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTCCCGAGTCGTATGAACATCGGt                                                                                                          >  1:225110/1‑143 (MQ=255)
                              aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                     >  1:150501/1‑143 (MQ=255)
                                gATGGCAGGTCGCCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATcc                                                                                                   >  2:960620/1‑143 (MQ=255)
                                      tggTCGTCACGGTAACAAGGGTGTAATTTCTAAGAGCATCCCGATCGAAGATATGCCGTACGATGACACGCGTACGCCGGCAGACATCGCACTGAACCACCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                             <  2:733037/142‑1 (MQ=255)
                                      aagtcgtcACGGTAACAACGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAATCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                             <  2:66938/141‑1 (MQ=255)
                                         tcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGCTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGCACCAGCTGGGCGTACCGTCTCGTATGAACCTCGGTCGGATCCTCGACAccc                                                                                          >  2:597902/1‑143 (MQ=255)
                                           gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACGCAc                                                                                        >  2:498941/1‑143 (MQ=255)
                                           gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCCAAAccccc                                                                                        >  1:561024/1‑141 (MQ=255)
                                           gtcACGCTAACAGATGTTTAATTTCTAAGATCAACCCGATCTAAGATATGCTTTACGATGAAAACGGTACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                        <  1:397727/143‑1 (MQ=255)
                                            tcACGGTAACAAGGGTGTAATTTCTACGATCAACCCGATCGAAGATATGCCTTACGCTGAGAACGGTACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGACCATCGGTCAGATCGTCGAAACCCGcc                                                                                       >  1:694770/1‑143 (MQ=255)
                                            tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACTGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAcc                                                                                       >  1:852395/1‑143 (MQ=255)
                                            tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAaaccaacc                                                                                       >  2:517439/1‑143 (MQ=255)
                                            tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAcc                                                                                       >  1:53214/1‑143 (MQ=255)
                                             cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                  >  1:568184/1‑131 (MQ=255)
                                             cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                  <  2:568184/131‑1 (MQ=255)
                                              aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGATGGGAGTACCGGCGCGTATGACCATCGGTCAGATCCTCGAAACCCACCTg                                                                                     >  2:940339/1‑143 (MQ=255)
                                                     cAAGTGTGTAATTTCTAAGATCAACCCGATCGAAGATCTGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                              <  1:32712/143‑1 (MQ=255)
                                                      aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCGTCGAAACCCACCGGGGTATGGc                                                                             >  2:140765/1‑143 (MQ=255)
                                                         ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                          >  1:480706/1‑143 (MQ=255)
                                                              aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTGCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                     <  1:1073745/143‑1 (MQ=255)
                                                               aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAGCCAGCTGGGCGTACCGTCTCGt                                                                                                                      >  1:140002/1‑93 (MQ=255)
                                                               aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAGCCAGCTGGGCGTACCGTCTCGt                                                                                                                      <  2:140002/93‑1 (MQ=255)
                                                               aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt                                                                                                                      >  1:139998/1‑93 (MQ=255)
                                                               aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt                                                                                                                      <  2:139998/93‑1 (MQ=255)
                                                                   cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGACTGGCTGCGAAAGGTATc                                                                >  2:263208/1‑143 (MQ=255)
                                                                     acgaccAACCCGCTCGAGGGTGGGGCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCgg                                                              <  1:400646/138‑1 (MQ=255)
                                                                      aGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGGAACCACCCCGGGTATGGCTGCGGAAGGTAGCGGc                                                             >  2:1075822/1‑143 (MQ=255)
                                                                        aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACCTCGGGCATATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                           >  1:744244/1‑143 (MQ=255)
                                                                         tCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTACGGCTGCGAAAGGTACCGGCGaa                                                          >  2:378962/1‑142 (MQ=255)
                                                                          cAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACa                                                         <  2:121762/143‑1 (MQ=255)
                                                                           aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                        >  1:587992/1‑143 (MQ=255)
                                                                            aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                       >  2:1088138/1‑143 (MQ=255)
                                                                             cccGATCGAAGATATGCCTTACGAAGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                      <  1:559763/143‑1 (MQ=255)
                                                                              ccGATCGAAGATATTCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTTTCGTATGAACATCGGTCCGATCCGCGAAACCGACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGac                                                     >  2:36914/1‑142 (MQ=255)
                                                                              ccGATCGAAGATATGCCTTACGATGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCAGAGTATGGCTGCGAAAGGCAGCGGCGACAAGAt                                                     >  2:902294/1‑143 (MQ=255)
                                                                              ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                     <  1:909051/143‑1 (MQ=255)
                                                                                 aTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                  >  2:58269/1‑143 (MQ=255)
                                                                                        atatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg                                           <  1:960620/143‑1 (MQ=255)
                                                                                         tatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                          >  2:311582/1‑143 (MQ=255)
                                                                                         gacgCCTTCCGATGAAAACGGTACGCCGGTAGACCTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                          <  2:862295/140‑1 (MQ=255)
                                                                                          atGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGcg                                         >  1:492539/1‑142 (MQ=255)
                                                                                          atGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCt                                         >  1:191874/1‑143 (MQ=255)
                                                                                            gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCCTGCTGa                                       >  1:1049829/1‑143 (MQ=255)
                                                                                               ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAGCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                    >  2:276988/1‑143 (MQ=255)
                                                                                               ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                    <  1:703159/143‑1 (MQ=255)
                                                                                                 aggATGGAAGCGGTACGCCTGTAGGCGTCGTACTGAGCCAGCTGGGCGTACCTTCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGAAGGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                  <  2:306364/141‑1 (MQ=255)
                                                                                                    aTGAAAACGGTACGCCGGTAGACATCGTCGCAACGCATCTGCGCGTACCGTCTCGTATGACCATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTGGGAAAGGTATCGGCGACAAGAACAACGCCACGCTGAAAcagcac                               >  2:602959/1‑142 (MQ=255)
                                                                                                      gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAgcagca                             >  2:133724/1‑143 (MQ=255)
                                                                                                      gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTACAGCTGCGAAAGGAATCGGCGACAAGAGCAACACCATGCGGAAACAgcaaca                             >  2:118280/1‑143 (MQ=255)
                                                                                                       aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGACCAACGCCATGCGCAAACAGCAGCaa                            >  2:1011062/1‑143 (MQ=255)
                                                                                                        aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGGACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAACGGTATCGGCGACACGATCAACGCCATGCTGAACCAGCAGCgag                           >  2:380735/1‑143 (MQ=255)
                                                                                                        aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                           <  2:418499/143‑1 (MQ=255)
                                                                                                               acacctgTAGCCTTCCTATTGAACCAGCCGCGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCTAAACCCACCTGGGTATGTCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                    <  2:808278/137‑1 (MQ=255)
                                                                                                               aCGCTGGTATAAATCGAACTGTACCAGCTGGGCGCACCGTCTCGTATGAAAATCGGTTAGACCCTCGAACCCCACCTAGGTAGGGCTGCTAAAGGTATCGGCGACTAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                    <  2:513129/143‑1 (MQ=255)
                                                                                                                 gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa                  >  1:220926/1‑143 (MQ=255)
                                                                                                                   ccggaGACGTCGTGCTGGGCCGGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaaa                <  2:1074152/139‑1 (MQ=255)
                                                                                                                     ttataCATCGTACTGAACCCGCTGGGCGTACCGTCTCTTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACt              <  2:112367/139‑1 (MQ=255)
                                                                                                                       aGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc            <  1:635016/143‑1 (MQ=255)
                                                                                                                           aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACCAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGa        >  2:856127/1‑143 (MQ=255)
                                                                                                                            tagTACTGAACCAGCTGGCCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa       <  2:276406/141‑1 (MQ=255)
                                                                                                                              gTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCCGATCCTCGAAACCCACCTGGGTGTGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGGAACAGCAGCAAGCAGTCGCGACACTGCTCGAAtt     >  1:76776/1‑143 (MQ=255)
                                                                                                                                 cTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAt  <  1:940339/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAT  >  NC_000913/4184407‑4184678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: