| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 96.5% | 170.5 / NA | 59 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (26/30); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
ATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132649‑2132926 | aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGCTCTGCATTTCACCGTGATTGATATGCAGGCTCTg > 2:1054480/1‑143 (MQ=255) cTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGTGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCg > 2:500194/1‑143 (MQ=255) ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCCCCGTGATTGATATGCAGGCTCTGCgcc > 2:47466/1‑141 (MQ=255) ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc > 1:953042/1‑143 (MQ=255) tAGAGGGTGAACAGTTGATATTTGTTGCTGGCGCGGTCGAAGTTGTCTTTGTGCTTGGGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 2:195814/143‑1 (MQ=255) tAGAGGGTCTTCCGCTGATATCTTTTGCTGGCGCGGTCGATTTTGTCTTTGTGCTTGGGGTAATAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 2:760177/143‑1 (MQ=255) ggTGAACAGCTGATATTTTTTGCTGGCGCGGCCGAATCCGCTTTCGTGCTTGCGGTAAAACTGGAAGTACCCGGAGAAGTTTTTCGGTGACGAGGTGATCTTCATTTCACCGCGATTGATCTGCCGGGTCGGCGTCGCCtctt > 2:951852/1‑143 (MQ=255) tAAACAGCTGATATTATTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGCAGTACCCGGAGCATTTTTTCGGTGGCGAGGTGATCTGCATTTCACCGTGATGGACATGCGGGCGCGGCGTCGCCtctgct > 2:957553/1‑143 (MQ=255) aagcAGCCGATTTTTTGTGCTGGCGCGGTCGAATGTGTCTTTGTGCTTGCGGTAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa < 2:191328/140‑1 (MQ=255) aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa < 1:8989/143‑1 (MQ=255) aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACTAGGTGATCTGCATTTCACCGTGATTGATTTGCAGGCTCTGCGTCGCCtcttcttct > 2:106174/1‑143 (MQ=255) aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTATCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt < 2:546127/143‑1 (MQ=255) gCTGATATTTTTTGCTCGCGCGGTCGAATTCGTCTTTGTGCCTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc < 1:443181/143‑1 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAGTTTCCa > 1:407759/1‑143 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa < 1:392105/143‑1 (MQ=255) gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAc > 1:764239/1‑143 (MQ=255) tatTTTTCGCTGGCGCGGTCGAATTTGTCTTTGTGCTCGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg < 1:969504/143‑1 (MQ=255) ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTACTccccc > 1:65262/1‑143 (MQ=255) tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCTCTTCTACTGTCCCCGGTTCGCCGGACTccaccac > 2:442359/1‑143 (MQ=255) tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACGGTTAGCCGTACTcaaccac > 2:840693/1‑143 (MQ=255) tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccac > 2:361184/1‑143 (MQ=255) gAATTTGTGTTTGTGGGTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacca < 1:47466/143‑1 (MQ=255) aTTTGGCTTTGTGCTTGCGGTAAAGGGGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc < 1:323886/143‑1 (MQ=255) tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt > 1:801778/1‑113 (MQ=255) tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt < 2:801778/113‑1 (MQ=255) tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGc < 1:608068/143‑1 (MQ=255) tttGTGCTTGCGGTGACAGTGGTAGTACCCGGCGAGTTTTTTCGGTGGCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGa < 2:48263/143‑1 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATGTGCATTTAACAGTGATTGATATGCAGGATCTGCGTCGCGTCTTCTACGTTCCACGCTTAGCCGCACGCCACCACCACC‑CGCACGa > 2:451502/1‑143 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCATGGTTCGCCGTACTCCACCACCATC‑CGCAGGa > 2:877608/1‑143 (MQ=255) tgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTAGGTGATGAGGTGATATGCATTTCACCGTGATTGATATGAAGGCTCTGCGTCGCCTCTTCTACTTTCCACCGTTAGTCGTAATCCACGCCCATCTCGC‑GGAAGAt > 2:599559/1‑143 (MQ=255) tgCTGGCGGTAAAAGGGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGAt < 2:847457/143‑1 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCGTC‑CGCAGGAAGATGt > 1:129422/1‑143 (MQ=255) ttcGGTATAAGTGGAGGTCGCCGGAGAATTTTTTCGGTGAGGAGGTGAGCTGCAGTGCACCGGGATTGATATGCAGGCGTTGCGTCGCCTCTTCCACTTTCCACGGTTCTCCGTACTCCACCACCATC‑CGCAGGAAGATGTCg < 2:1005985/141‑1 (MQ=255) gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGt < 1:1045746/143‑1 (MQ=255) gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGt < 1:951852/143‑1 (MQ=255) ggTAAAGGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATAGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTaa < 2:126493/143‑1 (MQ=255) aaaGTGGAAGTACCCGGAGAATTTGTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCt < 1:898377/143‑1 (MQ=255) aGTTGAACTACGCGGAGCACTTCTTCGGGGACGCGGTAACCTGCATTTCACCTTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCtgt < 1:500194/143‑1 (MQ=255) gTGGTAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCtgtg < 1:614398/143‑1 (MQ=255) gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGCAGATGTCGTAACCCtgcg > 2:449931/1‑141 (MQ=255) gTGCAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCtgtg < 2:128008/143‑1 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATC‑CGCAGGAAGATGTCGTGATGCTGTGc > 2:960259/1‑143 (MQ=255) cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt > 1:156403/1‑143 (MQ=255) gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGAGTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg < 2:787933/143‑1 (MQ=255) gttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCACCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc < 2:503885/142‑1 (MQ=255) tttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTCTCCATTAGGTATCGa > 2:575690/1‑143 (MQ=255) gggACGAGGTGATCTGCATTTCACCGTGAGTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 2:380098/141‑1 (MQ=255) tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaac < 1:818530/143‑1 (MQ=255) ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGCACAAACACTCtt > 2:418122/1‑143 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt < 1:367742/143‑1 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCTGCTTTCAGTTCGGTATCGAACCAACACCCTTTgc > 1:728853/1‑142 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTACCGAACCAACACTCTCTGaa > 2:299620/1‑143 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAACCCCTCTTTgca > 1:604272/1‑141 (MQ=255) tcggCATTTGACCGTGATTGATGTGCAGGATCTTCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATC‑CGCAGTAAGATGTCGTCATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa < 2:940916/140‑1 (MQ=255) atatGCAGGCTCTGCCTCTCCTCTTCTACTTTCCACGGTTCACCATACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc < 1:599559/143‑1 (MQ=255) tatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGCACAAACACTCTTTGAAACGCCATGCCCAGGTATAGccc > 1:1044051/1‑143 (MQ=255) cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCATGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt > 2:10612/1‑143 (MQ=255) aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt < 2:156403/143‑1 (MQ=255) aGGCTCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACCCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt < 1:1079871/143‑1 (MQ=255) | ATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATC‑CGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132649‑2132926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |