Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.92.2% 249.8 / 9.8 64pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base . (31/28);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009132,173,3620C.95.1% 238.9 / 8.8 64pseudogene (1/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (1/2);  major base . (31/27);  minor base T (0/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATT  >  NC_000913/2173221‑2173502
                                                                                                                                            ||                                                                                                                                            
aTTTCCACGATGCACGGCGACCGCCATCGCCGCGAAGAAGCCGATGGTGGCAAGACCGCCAAACGGCAGCACCTGATTACCCGCCACACCAACAGCCATTAAAATGGTGAGTGGGACAAAGACCAGGCTCGACGATaccaccg                                                                                                                                             >  2:319208/1‑142 (MQ=255)
 tttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgc                                                                                                                                            >  2:586658/1‑141 (MQ=255)
 tctCCACGAGGCACGGCGACCGCCATCGCCACGAGGAAGCCGAGGGTGGCAAGATCGCCAAACGGCAGCATCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgc                                                                                                                                            <  2:212553/141‑3 (MQ=255)
  ttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGCTCGCCAAACGGCTGCAGCTGTATACCCGGCACACAAAAAGCAATTAAAATGGTGAATGGGATAAAAATCAGGCTTGACGATaccacagac                                                                                                                                           >  1:1087368/1‑139 (MQ=255)
     cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCCCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAATGGGATAAAAATCAGGCTTGCCGATaccaccgccgta                                                                                                                                        >  1:66105/1‑137 (MQ=255)
              cGGCGACCGCCAGTGCCACCAAGACGCCGCTGGTGGCAAGAGCGCCAAACGGGAAGACCTGATTACCCCGCAGACAATCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCcagc                                                                                                                             <  1:594385/143‑1 (MQ=255)
                   aCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACAAACAGCAATTAAAATGATAAGAAGAATAAAAATAAGGCTTGACGATACGA‑‑CCGCCGTATGTCGCAGCGGCaa                                                                                                                        >  1:139381/1‑143 (MQ=255)
                    ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGCGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAc                                                                                                                       <  1:1228589/143‑1 (MQ=255)
                     cGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAACTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCCGCCGCTACg                                                                                                                      >  1:100791/1‑143 (MQ=255)
                      gCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCCCCGCCGTATGTCCCagctgccccgc                                                                                                                     >  1:443839/1‑138 (MQ=255)
                          tCGCCACGAAGAGGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGa                                                                                                                 <  2:716048/143‑1 (MQ=255)
                          tCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGa                                                                                                                 <  2:751042/143‑1 (MQ=255)
                            gCCACCAAGAATCCGATGTTCGCATGATTGTCATTCTGCAGCACCTGATTACCCGGCACACAAACAGCAGTTAAAATGGTGAGTGGGAGAAAAAGCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATc                                                                                                               <  1:634998/143‑1 (MQ=255)
                             ccACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCCACGCCGGACCa                                                                                                              >  2:108441/1‑143 (MQ=255)
                              cACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCaa                                                                                                             <  1:1227825/143‑1 (MQ=255)
                              cACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCaa                                                                                                             <  1:1101566/143‑1 (MQ=255)
                               aagaagaaGCCGATGGCGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg                                                                                                            <  1:190890/141‑1 (MQ=255)
                               aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg                                                                                                            <  1:954826/143‑1 (MQ=255)
                               aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg                                                                                                            <  1:809616/143‑1 (MQ=255)
                                cGAGGAAGCCGATGGTGGCAAGACCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAgg                                                                                                           <  1:888941/143‑1 (MQ=255)
                                cGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAgg                                                                                                           <  2:937408/143‑1 (MQ=255)
                                agaggaagCCGACGGTGGCAAGAGCGCCGAACGGAAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAACCAGGCTTGCCGATCCGA‑‑ACACCGGGTTTCCCAGCAGCAACGCCGGATCAAgg                                                                                                           <  2:710123/139‑1 (MQ=255)
                                       gCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCCCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa                                                                                                    <  1:610120/143‑1 (MQ=255)
                                           aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGGGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATccagga                                                                                                >  2:203673/1‑138 (MQ=255)
                                                ggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAGAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAATGCCAATCAGGAACTCCt                                                                                           >  1:481788/1‑143 (MQ=255)
                                                  cacgttCGCCACACTGTAGCACCTTATTACCCGCCCCACGAGCAGCAAGTCAAATGCTGAGTGGTATAAAGGTCAGCCTTGCCGTTCCATCCGCCGTATGTCCCTGCAGCAACGCCGGATCAAGGCCACTCAGGAACTCCTGa                                                                                         <  2:741785/138‑1 (MQ=255)
                                                      aTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc                                                                                     >  1:1090428/1‑143 (MQ=255)
                                                      aTCGCCAAACGGCAGCAACTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATAAGGCCTGCCGATACCA‑‑CCGCCGTACGTCCCAGCAGCAACGCCGGACCCAAGCCCATCAAGAACACCCGAccgc                                                                                     >  2:587567/1‑143 (MQ=255)
                                                         gCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGa                                                                                  >  1:747824/1‑143 (MQ=255)
                                                           cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGGACGCCTGACCGCCGAAc                                                                                >  1:205613/1‑143 (MQ=255)
                                                            aaaCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGGTCACGGCCCATCAGGAACTCCTGGCCGCCGACCt                                                                               >  1:61089/1‑143 (MQ=255)
                                                                  cagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCCGACCCCCGCACTTCGCCt                                                                         >  2:116288/1‑143 (MQ=255)
                                                                    gccacTGATTACCCGCCTCACAAGGAGCAATTAAAATGGCGAGTGGTATAAAAACCAGGCTTGCCGATACCA‑‑CCACCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGt                                                                       <  2:470760/139‑1 (MQ=255)
                                                                       ccTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGCTACAAATCAGGCTTGCCGATACCA‑‑CCGCCGGAGGGCCCAGCAGCAACGCCCGCTCAAGGCCAATCCGGCACCCCTGACCGCCGCCCTTCGCCTCTaaa                                                                    >  2:862934/1‑143 (MQ=255)
                                                                          gatggcccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGa                                                                 <  1:802445/138‑1 (MQ=255)
                                                                          gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAACCGa                                                                 >  1:1047857/1‑143 (MQ=255)
                                                                          gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGa                                                                 <  1:215152/143‑1 (MQ=255)
                                                                          gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGa                                                                 <  1:273032/143‑1 (MQ=255)
                                                                           aTTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAc                                                                >  1:1100609/1‑143 (MQ=255)
                                                                                ccGACACACAAACAGCAATTAAAATGGTTAGTTGTATAAAAATCATGCTTTACGATACCA‑‑CCGCCGTATGTCCCAGCAACAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGa                                                           <  1:361898/143‑1 (MQ=255)
                                                                                  cgCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCACGCTTGCCGATACTA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                         <  2:669207/142‑1 (MQ=255)
                                                                                     acacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGCAAACGACTACGAGCCTg                                                      >  2:833181/1‑143 (MQ=255)
                                                                                         aaaCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTCCACGACTACGGGCcttcttc                                                  >  1:360104/1‑142 (MQ=255)
                                                                                         aaaCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTa                                                  >  2:740140/1‑143 (MQ=255)
                                                                                          aaCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACAGAAGAGCAGCGCCTGTAAACGACTACGAGCCTGCTTAg                                                 >  1:1001398/1‑143 (MQ=255)
                                                                                            cAGCAAATAAAATGGTGAGTGGGATAAGAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCg                                               <  2:197615/143‑1 (MQ=255)
                                                                                              gCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                         <  1:1015979/131‑1 (MQ=255)
                                                                                              gCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                         >  2:1015979/1‑131 (MQ=255)
                                                                                              gCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                             >  2:776275/1‑143 (MQ=255)
                                                                                                 atgaaaaAGGCGAGGGAGACAGAAATCAGGCGTGCCGATCCCCCCGCCGTATGTCCCAGCAGCAGCGCCGGACCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATggg                                          <  2:208883/140‑1 (MQ=255)
                                                                                                  ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGACGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc                                         <  1:575284/143‑1 (MQ=255)
                                                                                                      aaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTa                                     >  2:1211009/1‑143 (MQ=255)
                                                                                                            gAGTGGGATAAAAATCAGGCTTTCCGATACCA‑‑CCGCATTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                               <  1:367532/143‑1 (MQ=255)
                                                                                                             aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGACGAACATAACAAGTAAACGACTACGAGCCAGCTTAGCGATGGGCGTTAAAccatc                              >  1:1246648/1‑143 (MQ=255)
                                                                                                              gTGGGATAAAAATCAGGCTTGCCAATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCACCTGTAAACGACAACGAGCCTGCTTAGCGATGGGCGTTAAAccatcc                             >  2:176599/1‑143 (MQ=255)
                                                                                                                gggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGCAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatccat                           <  1:116288/143‑1 (MQ=255)
                                                                                                                 ggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatg                          >  2:799851/1‑143 (MQ=255)
                                                                                                                  gATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGGTCACGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTACACCACTACGAGCCTCCTTATCGATAACCGTTAAAACAACCatga                         >  1:556016/1‑143 (MQ=255)
                                                                                                                  gATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTGCGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                         >  2:1160470/1‑143 (MQ=255)
                                                                                                                  gATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                         >  2:640241/1‑143 (MQ=255)
                                                                                                                   agaaacaTCCGGCTTGCCGATAC‑‑CTCCGCCGTATGTCCCCGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgat                        <  2:1069391/141‑1 (MQ=255)
                                                                                                                     aataaTCAGGCTTGCCGGTGC‑‑CTCCGCCGTATGTCCCAGCAGCGACGCGGGATCAGGGCCAATCAGGAACTCCGGACCGGCGAACGTCGCCTGTAAACGACTACGAGCCTGCGTAGCGATGGGCGTTAAACCATCCATGATgg                      <  2:63144/140‑1 (MQ=255)
                                                                                                                     aaaagTCAGTCTGGCCGATACCA‑‑CCGGCGTATGTCCCATCAGCAACGCCACAAAAAGTCCAATCCGGAACTCCTGTCAGCTGATCTTCGCCTGTATACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg                      <  2:77635/143‑1 (MQ=255)
                                                                                                                     aaaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTCCGAGCCTGCTTAGCGATGGGCGTTAACCCATCCATGATgg                      >  1:1163780/1‑143 (MQ=255)
                                                                                                                     aaaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGGTAAACCATCCATGCTgg                      >  2:1072232/1‑143 (MQ=255)
                                                                                                                        aagcAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGtt                   <  1:729523/140‑1 (MQ=255)
                                                                                                                              gCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGCGCCTGCTTAGCGATGGGCGGTAAGCCATGCCCGCTGGGTTTCATcg             >  1:1144211/1‑142 (MQ=255)
                                                                                                                                ttGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCCTTAAACCATCCACGATGGCTTCAATCAcc           >  2:251892/1‑143 (MQ=255)
                                                                                                                                ttGCCGATACCA‑‑CCGCCGGATGTACCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAcc           <  2:1051601/143‑1 (MQ=255)
                                                                                                                                           cccccGCCGTATGTCCCAGCGGCACCGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt  <  2:1269431/142‑1 (MQ=255)
                                                                                                                                            ||                                                                                                                                            
ATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATT  >  NC_000913/2173221‑2173502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: