Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,468 (G)5→6 intergenic (+48/‑39) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,4631.G88.6% 200.7 / 20.1 71intergenic (+43/‑44)ilvL/ilvXilvG operon leader peptide/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base . (4/4);  new base G (27/35);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

AGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCA  >  NC_000913/3950327‑3950598
                                                                                                                                          |                                                                                                                                          
aGCCCTTCTACGAGTGATTAGCCTGGTAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACGCCCGCACCGGAAGGGCggggggc                                                                                                                                    >  2:681418/1‑142 (MQ=255)
   ccTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACGAAGACCCCCGCACCGAAAGGTCCGGGGGgttt                                                                                                                                >  2:161544/1‑143 (MQ=255)
        tACGAGCGATTAGCCTTGTCGTGCTTAGCGTGGTGGTGATTATTATCCCACCGTGCGTGGCTGCACTTGGACGAGGACAGGCTGAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGgtttttttt                                                                                                                           <  2:849261/143‑2 (MQ=255)
         aCGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGAACAAGCCTAAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGgatttttttg                                                                                                                          >  2:507544/1‑141 (MQ=255)
           gAGTGATTAGTCTGGTCGTGAGTAGCGTGGTGGTGATTCTTCTCCCCCTCTGCGTGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCTTTAACGAACTAAGACTCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGAc                                                                                                                         <  2:508027/143‑1 (MQ=255)
           gAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGAc                                                                                                                         <  2:792516/143‑1 (MQ=255)
           gAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGAc                                                                                                                         <  2:400591/143‑1 (MQ=255)
                 ttAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTaca                                                                                                                   >  1:903024/1‑141 (MQ=255)
                   aGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTaaaaa                                                                                                                 <  1:1091443/143‑1 (MQ=255)
                    gCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATTAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGTTCCGGGGGGTTTTTTTTGACCTTAAAAAc                                                                                                                <  2:93826/143‑1 (MQ=255)
                    gCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCCGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAAc                                                                                                                <  1:853239/143‑1 (MQ=255)
                    gCCCGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAAc                                                                                                                <  1:977688/143‑1 (MQ=255)
                       tGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATa                                                                                                             >  1:1284979/1‑143 (MQ=255)
                         gttgTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAAc                                                                                                           <  1:1294524/140‑1 (MQ=255)
                         gTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGCCCTTAAAAAcgtgac                                                                                                           >  1:528313/1‑138 (MQ=255)
                         gTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAAc                                                                                                           >  1:799548/1‑143 (MQ=255)
                              gagtAGCGTGGTGGTGAGTATTATCCCACCGCGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAACAACATAACCgagg                                                                                                      <  1:391405/140‑1 (MQ=255)
                                  aGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                                                                  <  2:526918/143‑1 (MQ=255)
                                    cGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCACGCACGGAAAGGACCAGGGGGTTTTTATTTATCTGTAAAACATAACCGAGGAGCAgc                                                                                                >  1:475842/1‑142 (MQ=255)
                                    cGTGGTGGGGATTATTAGCCCACCGGGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGGCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                                                                <  2:448384/143‑1 (MQ=255)
                                       ggtggtgAGTAGGATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACaa                                                                                             <  1:126796/143‑1 (MQ=255)
                                        ggggtggtTATTATCCCACCGTGCGGGCCTCTGTGTTTGCGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                                                                            <  2:263109/141‑1 (MQ=255)
                                           gtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGCAAGGTCCGGGGGGCTTTTTTTCAACTTAAAAAAATAAACTAAGAGCAGAAAATGaa                                                                                         >  1:564253/1‑143 (MQ=255)
                                            tgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAAt                                                                                        >  1:63053/1‑143 (MQ=255)
                                             gATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGCATa                                                                                       >  1:527019/1‑143 (MQ=255)
                                             gATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATa                                                                                       >  1:477848/1‑143 (MQ=255)
                                              attattATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATaa                                                                                      <  2:1286820/143‑1 (MQ=255)
                                              attattATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGGTTTTTTTGACCTTAAAAACAAAACCAAAAAACAAACAATGAATaa                                                                                      >  1:1180353/1‑143 (MQ=255)
                                                tattctCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                                                                    <  2:252462/143‑1 (MQ=255)
                                                tattatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                                                                    <  1:885965/143‑1 (MQ=255)
                                                tattacCCCACCTGCCGCGGCTCCACTTGGACGATAAAAGGCTTAGAGATCAAGCCTTAACGAACTAACACCCCCGCACCGAAAGGTCCGGAGGGTTTTTTTTAACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                                                                    <  2:584422/143‑1 (MQ=255)
                                                           cGTGCGGGGCTGCACTTGGACGAGGAACGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACGGAAAGGGCCGGGGGGTTTTTTTTGACCCTGCAAACATAACCGTGGAGCAGACAATGAATAACAGCACAAAATTc                                                                         <  2:920886/143‑1 (MQ=255)
                                                            gcgCGGGGCTGCCCTGGGACGAGGAAAGGCTGAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt                                                                        <  1:1019460/141‑1 (MQ=255)
                                                             tGCGGGGCTGTAGTTGGACTAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg                                                                       <  2:1018352/143‑1 (MQ=255)
                                                             tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg                                                                       <  1:830631/143‑1 (MQ=255)
                                                                ggggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGttt                                                                    <  1:953063/143‑1 (MQ=255)
                                                                    ctccACTGGGACGAGGAAGGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACGGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                                                                <  1:1197538/140‑1 (MQ=255)
                                                                    cTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGACCTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTCAAAAACATAACCGAGGAGCAGACGATGAATAACAGCGCAAACTTGTGCTTCCCa                                                                >  2:599118/1‑143 (MQ=255)
                                                                    cTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                                                                <  2:434892/143‑1 (MQ=255)
                                                                      gCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGCAAATGAATAACAGCACAAGATGCAGGTTCTAAAg                                                              >  2:540174/1‑143 (MQ=255)
                                                                       cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGa                                                             <  2:1031325/143‑1 (MQ=255)
                                                                        aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAAGAACAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAt                                                            >  1:621549/1‑143 (MQ=255)
                                                                          ttGGACGAGGAAAGGCTTAGAGCTCAAGCCTGAACGAACTAAGACCCCGGAGAGGTTGTTCTCGAATGGCTTTTGCTGGCTTTAAAAACATAACCTTGGTGCAGACAGTTAATAGCAGCACAAAATTCTGTTTCTCAAGAGTc                                                          >  2:915899/1‑143 (MQ=255)
                                                                           tGGGCGAGGAAAGGCGTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCa                                                         <  1:468603/143‑1 (MQ=255)
                                                                           tGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCa                                                         <  1:22298/143‑1 (MQ=255)
                                                                           tGGACGAGGAAAGGCTTAGAGATCAAGCCGTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTGTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCa                                                         <  2:383953/143‑1 (MQ=255)
                                                                            ggATGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg                                                        <  1:1113795/143‑1 (MQ=255)
                                                                                  ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACggg                                                  <  1:915899/143‑1 (MQ=255)
                                                                                   gAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGCTTCTCAATCTTCAGGACgggg                                                 >  1:147708/1‑143 (MQ=255)
                                                                                      gggCTTAGAGATCAGGCCGTAGCGAATTAAGATCCACGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAAGCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac                                              <  2:54915/142‑1 (MQ=255)
                                                                                          ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGACAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACCTACACTCGTTGCGGACAATGAGTGACGTCCCAAAATTCTGTTTCTCACGATTCCGGACGGGGacctcac                                          >  1:476250/1‑143 (MQ=255)
                                                                                          ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGACCCAGGCCAGGGaactaac                                          >  2:1182171/1‑143 (MQ=255)
                                                                                          tgagagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaac                                          <  1:44374/141‑1 (MQ=255)
                                                                                           tAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTATTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaact                                         <  1:23469/143‑1 (MQ=255)
                                                                                             gaaaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTAt                                       >  2:523658/4‑143 (MQ=255)
                                                                                                aTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACCTAACCGCGGTGCAGACAATGAATAACAGCACAAAATTAAGAAACACAAGAATAAGGACGGGGAACTAACTATGaa                                    >  1:69385/1‑143 (MQ=255)
                                                                                                 tCAAGCGTTAACTAACTAAGACCCCCGCACCGGAAGGTCCGGGGGGTTGTTTTGACCTTAAAAACATAACCGCGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATg                                  <  2:262975/143‑1 (MQ=255)
                                                                                                    agcagtAACGAACGAAGGCCCCCGCCCGGAAAGGACCGGGGGTGTTTTGTCGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGgc                                <  1:681418/138‑1 (MQ=255)
                                                                                                    aGCCTTAACGAACGAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGgc                                <  2:728690/143‑1 (MQ=255)
                                                                                                       cTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAAGTAACTATGAATGGCGca                             >  1:759049/1‑143 (MQ=255)
                                                                                                       cTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGca                             >  1:23193/1‑143 (MQ=255)
                                                                                                         tAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTAAGGACGGGGAACTAACAATGAATGGCGcaca                           >  1:1127795/1‑143 (MQ=255)
                                                                                                          aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCACGATTCAGGACGGGGCACTACCTACGACTGGCCCACAg                          >  1:1021902/1‑143 (MQ=255)
                                                                                                          aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTAAGAGTGGCGcaccg                          >  2:1192918/1‑141 (MQ=255)
                                                                                                            cGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAgtg                        >  1:260284/1‑143 (MQ=255)
                                                                                                                 caaGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCCCAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAgtgggtgg                   <  1:161544/142‑1 (MQ=255)
                                                                                                                      cccccGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACAt              >  1:123073/1‑143 (MQ=255)
                                                                                                                        cccGCACCGAAAGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGc            >  1:828394/1‑143 (MQ=255)
                                                                                                                               cGAAAGGTCCGGGGGGATTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAAAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGCACTAGCTCTGAATGGCGCACCGTTGGTGGGCCATCCGTTggcg     >  1:454050/1‑140 (MQ=255)
                                                                                                                                 aaaGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGc   >  1:697237/1‑143 (MQ=255)
                                                                                                                                 aaaGGTCCGGGGGGTTTTTTTTACCTTAAAAACATAACCGAGGAGCAGCCAATGAATAACAGCACAAAATTCTGTTTCTCAAGCTTCAGGACGGGGAACTAACTATCAATGGCGCCCAGTGGGTGTTACATGCGTTGCGGGcg  >  2:680200/1‑142 (MQ=255)
                                                                                                                                  aaGGTCCGGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGca  <  2:260284/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCA  >  NC_000913/3950327‑3950598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: