Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G95.5% 193.1 / 1.8 66intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (37/26);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGA  >  NC_000913/3560317‑3560588
                                                                                                                                             |                                                                                                                                     
aTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGGcgcag                                                                                                                                    >  2:706775/1‑139 (MQ=255)
   aaCGGCGGCGGGCCTTGGGTGATGTGCGCCGCGTCGTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAt                                                                                                                                 <  2:746629/143‑1 (MQ=255)
    aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGAGTGTTGACCGGGCTGGAAGGGAGGGCCGCACCGCCATGATGGCGCAGGATc                                                                                                                                <  1:545567/143‑1 (MQ=255)
       gCGTCGGGCCATTGGGGAGTGGCTCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAgg                                                                                                                             <  1:930853/143‑1 (MQ=255)
       gCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGGACTGGAAGGCAGCGCCGCGACGCCAGGATGGCGCCGGATCAgg                                                                                                                             >  2:59766/1‑143 (MQ=255)
          tccactcATTGGGGGTGTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGttt                                                                                                                          <  1:34932/137‑1 (MQ=255)
             aGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCGGGTTTTGc                                                                                                                       >  1:121797/1‑143 (MQ=255)
               ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAGCGGGTTTTGccc                                                                                                                     >  1:428701/1‑141 (MQ=255)
                cATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGCCGCAGGATCAGGTTTTACTcc                                                                                                                    >  2:634380/1‑143 (MQ=255)
                cATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTcc                                                                                                                    >  2:276829/1‑143 (MQ=255)
                    ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGACACGGCGCGTTAACCGAACTGGAAGGCAGCGCCCACCCGCAATGATGGGGCAGGAACAGGTCTTGCTCCGCCa                                                                                                                >  2:553506/1‑143 (MQ=255)
                       gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCGGCGGCGCACCGCCATGCTGGCGCCGGGTCAGGTCTTGCTCCGCCTGCt                                                                                                             >  1:411242/1‑143 (MQ=255)
                       gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTccgcccgcc                                                                                                             >  2:235698/1‑142 (MQ=255)
                           tGCTCCGCCACGTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                         <  1:922421/143‑1 (MQ=255)
                              tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTACGCCAGCTCATTGAg                                                                                                      >  1:364340/1‑143 (MQ=255)
                               ccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAACAAGGTTTGCTCCGCCAGCTCATTGAgg                                                                                                     >  2:837452/1‑143 (MQ=255)
                                  ccACTATGCGGGCGATGCGCTCTTATTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                  <  1:145473/143‑1 (MQ=255)
                                      tttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCACACCGCCATGATGACGCAGGATCAGGATATGCTCCGCCACCGCATTCAGCTCgcggc                                                                                              >  1:613455/1‑143 (MQ=255)
                                        tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa                                                                                            <  1:1018802/143‑1 (MQ=255)
                                          cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTAGCGGCGAAt                                                                                          >  1:69880/1‑143 (MQ=255)
                                           gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGTGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                         <  1:689526/143‑1 (MQ=255)
                                              cgctGCGCTCCTGTTCTTCGCTCTGTGTGGCCCTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCACCATGATGGCCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTc                                                                                      <  1:421242/140‑1 (MQ=255)
                                                 tGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATGAAAGGATGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                   >  2:566035/1‑143 (MQ=255)
                                                   cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                 >  1:100902/1‑143 (MQ=255)
                                                    gcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCGCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATGGAGGTGGCGGCGAATGGTCTGCggg                                                                                >  1:707438/1‑143 (MQ=255)
                                                    gcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGGTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                >  1:100513/1‑143 (MQ=255)
                                                    gcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCCCGGCGACTAGTCTCCggc                                                                                >  1:59497/1‑142 (MQ=255)
                                                     ctctTTGTGTTCGGGGTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                               <  2:181407/143‑1 (MQ=255)
                                                       ctTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTAACCGAACTGGAAGTCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTCTTGCTCCGACAGATCATTAAGGTCGCGGCGAGAAGTCTGAGGGCTg                                                                             >  1:677235/1‑143 (MQ=255)
                                                       ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTg                                                                             >  1:652965/1‑143 (MQ=255)
                                                       ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAGTAGTCTGCGGGCTg                                                                             >  1:52802/1‑143 (MQ=255)
                                                       ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGACGGCGCAGGATCAGGTTGCGCTCCGCCCGCTCATCGAGGTCGCCGCGAATCGTCTGCGGGccg                                                                             >  2:289957/1‑141 (MQ=255)
                                                        tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCGTTGAGGCCGCAGCGAATAGTCGGCGGGCTGa                                                                            >  2:1066227/1‑143 (MQ=255)
                                                        tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGa                                                                            >  1:530686/1‑143 (MQ=255)
                                                         ttttCTTCGTTCTGGTTGGCCTTTCGATCGTGCCACGGCGTGTTATCCGATCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                           <  1:686246/143‑1 (MQ=255)
                                                          tttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                          >  2:827356/1‑143 (MQ=255)
                                                               tttgtttGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTGTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                                     >  2:170300/7‑143 (MQ=255)
                                                                 ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTAGGCGGGCTGCGGGGGAAAt                                                                   >  2:1208690/1‑143 (MQ=255)
                                                                    cTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                                                                <  1:1094827/143‑1 (MQ=255)
                                                                        atGGTCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGCCATCGCGGTTTATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                            <  2:275906/142‑1 (MQ=255)
                                                                                  gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTAGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGCGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                  >  2:720488/1‑143 (MQ=255)
                                                                                    tCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAAGTCGCGGCGAATAGTCTGTGGGCTGACCGAAAAATGCGCTAACAGCTCTTAGGt                                                >  2:155563/1‑143 (MQ=255)
                                                                                      gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGCCGCAGGCTCCGGTTTTCCTCCGCCAGCTCATTGAGGTCGCGGCGAATAAACAGCGGGCTGACGGAAAAATGATCTACCAGCTCTACGGTat                                              >  1:407579/1‑142 (MQ=255)
                                                                                         tcACGGCGTGTTAACTGTACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                           <  1:5191/142‑1 (MQ=255)
                                                                                          cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc                                          >  1:566308/1‑143 (MQ=255)
                                                                                          cACGGCGTGTGAACCGAACTGTAATGTATCGCCGCACCGCCATGATAGCGCAGAATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc                                          <  1:386687/143‑1 (MQ=255)
                                                                                           aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa                                         >  2:691117/1‑143 (MQ=255)
                                                                                           aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTGGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCGCTTCGGTGCTGACa                                         >  2:782672/1‑143 (MQ=255)
                                                                                                gtgtTGACCGAACTGGCAGGCAGCGCCGCGCCGCCATGATGCCGCAGCAGCAGGTTTTGCTCCGCCAGCTGCGTGAGTTCGCGGCGGAAAGTCCTCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                                    <  2:1260374/143‑1 (MQ=255)
                                                                                                gtgtTAACCGAACTTTGTTCCATCGCCACGTCGCCATTATGCCGCAGTATCAGGTTTTTCTCCGCCGGCTCATTGAGCTCGCTGCGAATAGTCTGCGTGCTGACGGAGAAATGCTCTACCAGCTCTTCTGTACTGACATAAcc                                    <  2:744135/143‑1 (MQ=255)
                                                                                                gtgtGAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                                    <  2:289219/143‑1 (MQ=255)
                                                                                                 agtTAGCCGAACGGGAAGGCAGCGCCGCACCGCCGTGAGGGCGCGGGGTCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                   <  2:247556/142‑1 (MQ=255)
                                                                                                  gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                  <  1:639818/143‑1 (MQ=255)
                                                                                                      aCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCctcctg                              >  1:1242764/1‑143 (MQ=255)
                                                                                                       ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                             >  2:1082924/1‑143 (MQ=255)
                                                                                                       ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTGGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctgc                             >  1:884397/1‑142 (MQ=255)
                                                                                                        cGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACACTGCTGta                            >  2:297312/1‑142 (MQ=255)
                                                                                                          aaCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCGGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                          <  1:30491/143‑1 (MQ=255)
                                                                                                           aCTGTAAGGCAGCGCCGCACCGCCATGACGGCGCAGGATCAGGTCTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTaa                         <  2:734684/143‑1 (MQ=255)
                                                                                                           aCTGGAGTGCAGCGCCGCACCGCTATGATGGTGCAGGATGAGGTTTTGCTCTGCCAGTGCATGGATGTCGCGGCGAATAGTGTGCTGGCTGACGGAGAAATGCTCTACCATTTCTTCGGTACTGACATAACCCTGCTGTTTaa                         <  2:515671/143‑1 (MQ=255)
                                                                                                            cTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGGTCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                        <  2:243875/143‑1 (MQ=255)
                                                                                                            cTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                        <  1:837452/143‑1 (MQ=255)
                                                                                                                   gCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                 >  1:2250/1‑143 (MQ=255)
                                                                                                                     aGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGCTTAACCAGTTCGat               >  1:314373/1‑143 (MQ=255)
                                                                                                                         ccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata           <  1:566035/143‑1 (MQ=255)
                                                                                                                                  cATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCATGCTGTTTAACCAGTTCGATAATACCGTTGTGa  <  1:827356/143‑1 (MQ=255)
                                                                                                                                  cATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGa  <  1:697208/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                     
ATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGA  >  NC_000913/3560317‑3560588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: