Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA98.5% 189.6 / NA 66P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (33/31);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184406‑4184681
                                                                                                                                         |                                                                                                                                          
gactgTTAAACAGCGTATGTAGCCTGGTGAGAAGATGGCAGGTCGTCACGGGAACAAGGGTGTAATTTCTAAGGTCAACCCGATCGAAGATATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                       <  2:127391/139‑1 (MQ=255)
  cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                     <  1:405290/143‑1 (MQ=255)
    gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGCAGACATCGTACTGCACCAGCTGGGCGt                                                                                                                                   >  2:717160/1‑143 (MQ=255)
      aaaccGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                                 <  1:67169/139‑1 (MQ=255)
          cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTCGACATCGTACTGAACCAGCTGGGCCTCCCGtc                                                                                                                             >  1:350235/1‑143 (MQ=255)
             cGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACACGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                          <  2:1009241/143‑1 (MQ=255)
                aTCCAGCCTGGTGACAAGATGGCAGGTCGTCGCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGCACTGAACCAGCTGGGCGCACCGTCTCGTAt                                                                                                                       >  2:386550/1‑143 (MQ=255)
                   cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCCCCAAGATGGAAGACACGCCTTAAGATGAAAAAGGAACGCCGGAAGACATCGAACTGAACCAGCAGGGCGTACCGTCTCGTATGaa                                                                                                                    >  1:481894/1‑143 (MQ=255)
                     gCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGGACa                                                                                                                  >  2:134068/1‑143 (MQ=255)
                        tcgTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               <  1:1129576/141‑1 (MQ=255)
                              gtaactGGCTGGTCGGCACGGGAACAAGGGTGTAATGTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGa                                                                                                         <  2:557777/138‑1 (MQ=255)
                                   tGGCGGGTCGTCACGGTAACAAGAGTGTAATTTCGAAGGTCAACCCGATCGGCGATATTCCTTACTATGACAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                    <  2:1119617/143‑1 (MQ=255)
                                     gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACACCGGCAAGATCCTCGa                                                                                                  >  2:247396/1‑143 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                <  2:226280/143‑1 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                >  1:267024/1‑143 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                <  1:1215115/143‑1 (MQ=255)
                                        ggTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAc                                                                                               <  2:1265870/143‑1 (MQ=255)
                                            gacACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                           <  1:93966/141‑1 (MQ=255)
                                             tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATCTGCCTTACGATGAAAACGGTACGCCAGTAGCCATCGTACTGCACCAGCTTGGCCTACCGGCTCGTATGAACAACGGTAAACACCTCGACCCCCAcc                                                                                          >  2:165814/1‑143 (MQ=255)
                                               aCGGTAACAAGGGTGTAATTTCTAAGATAAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGAGTACCATCTCGTACGAAAGGCGGAAAGATCATCAAAacacacaag                                                                                        >  2:314644/1‑140 (MQ=255)
                                               aCGGTAACAAGGACGTAATTTCTCAGATCAACCCGACCGACCATATGCCTTACGATGAAAACGGTACACCCGCAGACATCGCACGGACCCCGCCGCGCGTACCGTCTCATACGCACACCGCCCCAAACCTCGAAAcccccccc                                                                                        >  2:1280095/1‑141 (MQ=255)
                                                    cacactggTGGAATTTCTAAGATACACCCGATCGCAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                                   <  2:298566/137‑1 (MQ=255)
                                                      cAAGGGTGTAATTTCTAAGATCAACCCGAACTGCGATAAGCCGTACTATGAAAACGGTACGCCGGTAAACAGTGAACACGAGCTACTCGACTTTCCTTCTCGGATGAACGTCGGTCAGACCCGCCTAACCCACCTCGGTAtcg                                                                                 >  2:327157/1‑141 (MQ=255)
                                                       aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCgggggcgggc                                                                                >  2:186645/1‑137 (MQ=255)
                                                         gggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAGCGGCTCGTATGAACATCGGTCGGAGCCTCGAGGCCGACCTCGGTATGGCTg                                                                              >  1:1034237/1‑143 (MQ=255)
                                                         gggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCAGTCAGATCCTCGAAACCCACCTGGGTATGGCTg                                                                              >  1:1196708/1‑143 (MQ=255)
                                                         gggTATAATTTCTAAGATCAACCCGATCGATGATTGGCCTTACGATGACAACGGTCCGCCGGTAGACATCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGACACCGACCTGCGCATGGCTg                                                                              >  2:390632/1‑143 (MQ=255)
                                                              taaggacTAAGAGCAACCCGATCGAAGATAGGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  1:1134863/137‑1 (MQ=255)
                                                               aaTTTCTAAGATCAGCCGAACCGAAGATATGCCTTACGATGAAACCGGTACGCCGGTAGACATCGTACTGACCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                        <  2:761816/143‑1 (MQ=255)
                                                                aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATCGGCCAGACCCTCGAGACCCACCTGGGTATGGCTGCGCAAgg                                                                       >  1:187910/1‑143 (MQ=255)
                                                                 tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGt                                                                      >  2:1142018/1‑143 (MQ=255)
                                                                    cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc                                                                   <  1:1248241/143‑1 (MQ=255)
                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACGTCGGTCAGATGCTCGAAAGCGACGTGGGTATGGCTGGGAAAGGTATCg                                                                  >  1:492651/1‑143 (MQ=255)
                                                                      aaGATCTACCCGAGCGAAGATATGCCTTACGATGAAAGCGGTTCGGCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCgg                                                                 <  2:350235/143‑1 (MQ=255)
                                                                            aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTCGTGCAGGGAAAGGGATCGGCGACaa                                                           >  2:6690/1‑143 (MQ=255)
                                                                             aCCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                          <  1:191490/143‑1 (MQ=255)
                                                                             aCCCGAGCCAAGATACGCCTTACGCTGCCACCGGTACGCCGGTAGACAGCCTTCTGAACCAGCCGGGCCTACCGACTCGTATGAACATCGGCCAGAATGCCGTTATGGTTTTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                          <  2:275659/143‑1 (MQ=255)
                                                                                 gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCa                                                      >  1:783073/1‑143 (MQ=255)
                                                                                  ctCGAAGATATGCCTTACGATGAAATCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                     <  2:46654/142‑1 (MQ=255)
                                                                                   tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    <  1:820348/143‑1 (MQ=255)
                                                                                   tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    >  1:1245411/1‑143 (MQ=255)
                                                                                            tGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGGATGGCTGCGAAAGGTACCGCCGACAAGAGCAACCCCCAGCTg                                           >  2:46114/1‑143 (MQ=255)
                                                                                             gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGa                                          >  1:335571/1‑143 (MQ=255)
                                                                                              ccTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaa                                         >  2:996023/1‑143 (MQ=255)
                                                                                              ccTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAAATAGGTCAGATCCTCGAAACCCACCTGAGTATGGCTGAGAAAGGTATCAGCGACAAGATCAACGCAATGCAGaa                                         >  2:774118/1‑143 (MQ=255)
                                                                                                ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTATAGGCCACAAGATCAACGCCATGCTGacaa                                       >  2:97021/1‑140 (MQ=255)
                                                                                                  aCGATGAAGACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAGCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                     <  1:707123/143‑1 (MQ=255)
                                                                                                    gatgatAACGTGACGCCGGTAGACTGCGTACTGAACCAGCTGGGCGTTGCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                   <  2:448000/143‑1 (MQ=255)
                                                                                                    gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                   >  1:912352/1‑143 (MQ=255)
                                                                                                        aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATCAACATCGGTCAGATCCTCGAAACCCACCTGGCTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACggccgcca                               >  2:1154638/1‑141 (MQ=255)
                                                                                                         aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                              <  1:1094907/143‑1 (MQ=255)
                                                                                                          aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAACGGTCAGAACCTCGAAACCCACCTGGGGATGGCTGCGAAAGGTATCGGCGACAAGCACAACGCCAGGCTGAAACAGCAGCaaga                             >  2:640491/1‑143 (MQ=255)
                                                                                                               tACGCCGGTAGACATCGTCCTGAACCAGCTGGGCGGACCGTCTCGTATGAACACCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                        <  1:1004494/143‑1 (MQ=255)
                                                                                                                 cGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGACGCCAAGATCAACGCCATGCTGAAACCGCAACAACCAGAcgcg                      >  2:497661/1‑143 (MQ=255)
                                                                                                                  gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa                     >  2:379265/1‑143 (MQ=255)
                                                                                                                     ggTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCCTGCTGAAACAGCAGCAAGAAGTCGCGAAAc                  >  1:1148726/1‑143 (MQ=255)
                                                                                                                      gTAGACATCGTACTGATCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACt                 <  2:199787/143‑1 (MQ=255)
                                                                                                                       tAGACATCGTACTCAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTg                <  1:1271850/143‑1 (MQ=255)
                                                                                                                       gaGACATCGTACTGAACCAGCTGGGCGTTCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTg                <  2:696521/142‑1 (MQ=255)
                                                                                                                            aTCGTACTGAACGAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCAGGGTATGGCAGCGAAAGGTATCCCCGACACCAGCAACGCCATGCTGAAACAGCAGCAAGAAGTAGCGGAACGGcgcgg           >  1:48517/1‑142 (MQ=255)
                                                                                                                            aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGGGa           >  1:647388/1‑143 (MQ=255)
                                                                                                                              cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt         <  1:1000285/143‑1 (MQ=255)
                                                                                                                              cGTACTGAACCAGCTGGGCGTACCGCCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt         <  1:791339/143‑1 (MQ=255)
                                                                                                                                 aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAACGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa      <  1:835961/143‑1 (MQ=255)
                                                                                                                                 aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATACAAAAACAGCAACAAAAAGTCGAGAAACAGCGCGAAttaa      >  1:580687/1‑141 (MQ=255)
                                                                                                                                     taCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCa  <  1:1030093/142‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184406‑4184681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: