Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA97.1% 201.1 / ‑6.5 69P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (26/40);  total (26/43)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA  >  NC_000913/4184404‑4184677
                                                                                                                                           |                                                                                                                                      
cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCt                                                                                                                                     <  1:804173/143‑1 (MQ=255)
  ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                   >  1:295134/1‑143 (MQ=255)
   gCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTggg                                                                                                                                  >  1:773066/1‑143 (MQ=255)
    cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                 <  2:1164900/143‑1 (MQ=255)
    cGGCTAAACGCCGAATCCAGCCTGGTGACACGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCTATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                 <  1:366939/143‑1 (MQ=255)
       ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                              >  1:289931/1‑143 (MQ=255)
        tAAACTCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATAGTACTGAACCAGCTGGGCGTAc                                                                                                                             >  2:784578/1‑143 (MQ=255)
        tAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                             <  1:827668/143‑1 (MQ=255)
         aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATGTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGACAACGGCACGCCGGTCGACCTCGTACTGAACCAGCTGCGCCTAcc                                                                                                                            >  2:27065/1‑143 (MQ=255)
           aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                          >  1:713046/1‑143 (MQ=255)
                  aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                   <  1:720752/143‑1 (MQ=255)
                   tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGGACGCCGGTAGACATCGTCCTGAACCAGCTGGGCGTGCCGTCTCGTATg                                                                                                                  >  2:936846/1‑143 (MQ=255)
                     cAGCCCGGTGACAAGAGGGCAGTTCGGCACGGTAACCAGGGTGCAAGTTCTAAGATCAACCCGATCGAAGATATGCCTGACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                <  1:23727/143‑1 (MQ=255)
                      agctcggTGACAAGTTGGCACGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAc                                                                                                               <  2:631433/138‑1 (MQ=255)
                        ccTGGTGACAAGATGGCAGGTCGTCACGGAAACAAGGGTGTACTTATCAACGCGGCCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAt                                                                                                             <  1:855602/143‑1 (MQ=255)
                         cTGGTGACAAGATGGCAGGTCGTCACGGGAACAAGGGTGTAACTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATc                                                                                                            <  1:54797/143‑1 (MQ=255)
                             tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                        >  2:159080/1‑143 (MQ=255)
                              gACAAGATGGCAGGTCGTCACGGGAACAAGGGTGTAATCTCTACGTTCCACCCCATCGAAGATGCGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCa                                                                                                       <  1:44359/143‑1 (MQ=255)
                               aCAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCATCTGGGCGTACCGTCTCGTACGAACATCGGTCAg                                                                                                      >  2:348511/1‑143 (MQ=255)
                                  aGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATc                                                                                                   <  1:368610/143‑1 (MQ=255)
                                     tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                <  1:1099082/143‑1 (MQ=255)
                                     tGGCAGGTCGTCACGGTAACAAGGGGGTGATTTCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                <  2:468149/143‑1 (MQ=255)
                                        cAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCATCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaa                                                                                             <  1:804609/143‑1 (MQ=255)
                                         aGGTCGTCCGGGTAACCAGGGTGTAATTTCTAAGATCAACCCGATCGAAGGTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                            <  1:1128057/143‑1 (MQ=255)
                                         aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAGCGTTCAGATCCTCGaac                                                                                            >  1:484027/1‑142 (MQ=255)
                                         aGGGCGTCACCGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                            <  2:968233/143‑1 (MQ=255)
                                             cgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCa                                                                                        <  1:1091086/143‑1 (MQ=255)
                                             cgtcACGGCACCAAGGGTGTAGTGTCTACGATCAACCGGATCGAATATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCa                                                                                        <  1:517362/143‑1 (MQ=255)
                                              gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACACAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGCTCCCCGGAAcaccc                                                                                       >  2:665856/1‑139 (MQ=255)
                                               tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGCTAAAGCATCAGTTTGACACGGTACGCAGTCACACATCGTACGGAACAACCGCGGAGAACCGACTAGTAGGAACATCAAACAGATCCTAGAAACCCaac                                                                                      >  2:141162/1‑141 (MQ=255)
                                               tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAGGAACATCGGTCAGATCCTCGAAACCCAcc                                                                                      >  1:196508/1‑143 (MQ=255)
                                                     tAACAGGGGTGTCATTTCTAAGATCAACCCGGTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTa                                                                                <  2:673124/143‑1 (MQ=255)
                                                      aaCAAGGGTGTAAGTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                               <  2:361702/143‑1 (MQ=255)
                                                      aaCAAGGGCGGAATTCCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGCACCAGCTGGGCGTACCGTGTCGTATGAAGATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                               <  1:839117/143‑1 (MQ=255)
                                                        cAAGCGTGTCATTTCTAAGCTCAACCCGATCGAAGATATGCCTTACGACGAAAACGGTACGCCGGTAGACATCGTACTGCTCCCCCTGGGGGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                             <  1:674930/143‑1 (MQ=255)
                                                         aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGATCCGCGAAACCCACCCGGGCACGGc                                                                            >  2:423306/1‑143 (MQ=255)
                                                           gggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTg                                                                          >  2:1052655/1‑143 (MQ=255)
                                                            ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                         <  1:702260/143‑1 (MQ=255)
                                                               gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaa                                                                      >  2:1102644/1‑143 (MQ=255)
                                                                 aaTGTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTGCTGAACCGTCTGGGCGTCCCCACACGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                    <  2:834697/143‑1 (MQ=255)
                                                                  aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCACGAAACCCACCTGGGTATGGCTGCGAAAgg                                                                   >  2:857060/1‑143 (MQ=255)
                                                                   tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTGTCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGt                                                                  <  1:321476/143‑1 (MQ=255)
                                                                                cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCGACCTGGGTATGGCTGCGAAAGGGATCGGCGagcaga                                                     >  1:78261/1‑138 (MQ=255)
                                                                                 ccGCTCGAAGATATGCCTTACGAGGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                    <  1:507500/143‑1 (MQ=255)
                                                                                  gtaTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                   <  2:1023480/141‑1 (MQ=255)
                                                                                  ctaTCGATGATATGCCCTACGATGATAAGGCTACGGCCGTAGACATCGGACTGAACCAGCTGGGCGTACCGTCTAGTATGCACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                   <  1:751526/141‑1 (MQ=255)
                                                                                  cGATCGAAGACATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                   <  2:318437/143‑1 (MQ=255)
                                                                                   gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCa                                                  <  2:773066/143‑1 (MQ=255)
                                                                                     tCGAAGATAGCGCTTACGCTGAACCCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGGACCGTCTCGTATGAACATCGGTCAGACCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                <  1:565559/143‑1 (MQ=255)
                                                                                        aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACCTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACAAGGGGACGGGATGGAAAGGTATCCGCGACAAGAGCAACGcc                                             >  2:514929/1‑143 (MQ=255)
                                                                                        aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                                             >  1:923791/1‑143 (MQ=255)
                                                                                            tatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                         <  1:401738/143‑1 (MQ=255)
                                                                                            tatGCCTTACGAGGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                         <  1:256037/143‑1 (MQ=255)
                                                                                                 cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGGCGGGGATCTGGGAGAAGATCAACGCCATGCTGaaa                                    >  1:910598/1‑143 (MQ=255)
                                                                                                    aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTCTGGCTGCGAAAGGTAACGGCGACAAGATCAACGCCATGCTGAACcag                                 >  2:542729/1‑143 (MQ=255)
                                                                                                    aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                 <  2:295134/143‑1 (MQ=255)
                                                                                                     cGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGCCCCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGCGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                <  1:990510/143‑1 (MQ=255)
                                                                                                      gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                               <  1:1102644/143‑1 (MQ=255)
                                                                                                      gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                               >  1:875088/1‑143 (MQ=255)
                                                                                                           aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACAGTCTCGTATGAACATCGGTCAGATCCTCGAGACCGACCTGGGTATGGCTGCGAAAGGGGTCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCcag                          >  1:455853/1‑143 (MQ=255)
                                                                                                            aaCGGTACGCCACTAGACCTCGTACTGAACCATCTGGCCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                         <  2:760432/143‑1 (MQ=255)
                                                                                                             ccGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAGCGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                        <  2:645080/142‑1 (MQ=255)
                                                                                                             aCGGTATGCTGTTAGACTTCGTACTGATCCAGCTGGGCGTACCGTCTCGTATGAACATCTGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                        <  2:1048340/143‑1 (MQ=255)
                                                                                                                      cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaag               <  1:286599/143‑2 (MQ=255)
                                                                                                                        gTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACt             <  1:421600/143‑1 (MQ=255)
                                                                                                                            aCATCGTACTGATCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc         <  1:907682/143‑1 (MQ=255)
                                                                                                                            aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGGCAGATCCTCGAAACCCACCTGGGGGTGGCTGCGAAAGGTATCGGCGACAAGCTCAACGCCATGCGGAACAAGCAGCAAGAAGGAGCGAAACTgcgc         >  1:381134/1‑143 (MQ=255)
                                                                                                                              aTCGTACTGAACCAGCTGGGCGTACGGTCTCGTAGTAGCGGCGGGCAGATCCTCTAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGACACTGCGCGa       >  1:551327/1‑143 (MQ=255)
                                                                                                                                   aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa  <  1:84693/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA  >  NC_000913/4184404‑4184677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: